Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

Diamond synthesis from a system of chromium-carbide and Ni70Mn25Co5 alloy

In order to ascertain the mechanism of interaction between carbide and metallic catalyst and formation of diamond under high pressure and high temperature, and find a new method to synthesize diamond with special properties, it is necessary to investigate the reaction behavior of different carbides and metallic solvent_catalysts under high pressure and high temperature. A system of Cr-3C-2 powder and Ni 70Mn 25Co 5 alloy in weight ratio of 1∶6 was treated under 6 0 GPa and 1 500℃ for 20, 30 or 60 min respectively. X_ray diffraction of the samples indicated the Cr 3C 2 decomposed partially after high pressure and temperature treatment, and Cr 7C 3, Cr and diamond formed respectively. There was not any trace of graphite in the samples. The result suggested that the separated carbon atoms could form diamond directly without conversion process of graphite into diamond. The observation of SEI, WDX and EDX also showed that diamond crystals were synthesized in the system, which have perfect surfaces and shapes, with the average grain size of about 40 μm. The properties of the crystals are being investigated.     

U形渠道水跃的试验研究

从理论上推导了Ｕ形渠道的水跃方程。通过模型试验研究了Ｕ形渠道水跃的水流流态和流速分布,测量了跃前、跃后水深和水跃长度,提出了共轭水深、水跃长度和水跃消能率的计算公式。研究表明,Ｕ形渠道的水跃有良好的消能效果,其消能作用优于矩形渠道和梯形渠道,接近于三角形渠道。     

多沙水源取水泵站正向前池边壁型式及圆形进水池尺寸的确定

通过理论分析、模型试验和对现有多沙水源取水工程的调查,研究并给出了正向前池边壁的合理曲线型式和圆形进水池尺寸的确定方法。研究结果可应用于实际工程。     

Construction of High Level Expression Cell Lines of Erythropoietin *

Ｅｒｙｔｈｒｏｐｏｉｅｔｉｎ（ＥＰＯ）ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｒｅｇｕｌａｔｏｒｙｆａｃｔｏｒｓｄｕｒｉｎｇｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｍａｍｍａｌｉａｎｅｒｙｔｈｒｏｃｙｔｅ,ａｃｔｉｎｇｍａｉｎｌｙｏｎｔｈｅｅｒｙｔｈｒ...     

晋祠灌区农业节水灌溉分析

本文从节水灌溉概念出发,结合晋祠灌区的实际情况,在对种种上节水措施分析的基础上,为晋祠灌区的发展提供了一条可持续发展的道路。     

城市水资源水环境系统多阶段灰色动态仿真模型

本文依据城市人口,经济,水资源和水环境之间的相互影响,相互制约关系,建立了城市水资源水环境系统阶段灰色动态仿真模型,以预测不同仿真方案下城市水资源与水环境系统的变化。     

水分在季节性冻土中的运动

季节性冻土是一种冰水共存的特殊土水体系,在分析冻土中水分运动的驱动力及其影响因素,冻土介质的物理特性（热特性和水力特性）及其参数测定方法的基础上,综述了水分在冻土中运动的基本规律及定量研究水分运动的试验与数值模拟方法。     

矿山排水泵站微机通信测控系统

针对目前平庄矿务局元宝山露 天矿的实际情况,利用ＭＣＳ－９６系列单片机成了矿山排水泵站微机通信测控系统。该系统由调度端和终端站组成,调度端与终端站之间的信息伟递采用无线通讯方式。且具有多机通信功能,故系统具有高可靠性和高性能价格比。     

FD-TRT耐热逆转录酶的特性分析

从嗜热细菌ＦＤ３００９中克隆并分离纯化得到的耐热逆转录酶（ＦＤ－ＴＲＴ）,逆转录反应在６５℃高温下进行,可克服常温逆转录酶的许多缺点,以酵母核糖体ｒＲＮＡ为模板,以与２５５和１８Ｓ ｒＲＮＡ匹配的寡聚脱氧核苷酸为引物,探明了ＦＤ－ＴＲＴ的最适反应条件： ２５ｍｍｏｌ／Ｌ Ｔｒｉｓ－ＨＣｌ（ｐＨ ８．５,２５℃）,２５ ｍｍｏｌ／Ｌ（ＮＨ４）２ＳＯ４,２ ｍｍｏｌ／Ｌ ＭｎＣｌ２,１００ μｇ／ｍｌ明胶．５ ｕｎｉｔ ＲＮａｓｉｎ,２５０ ｍｍｏｌ／Ｌ ４种 ｄＮＴＰ, １μＣｉ３ Ｈ－ｄＣＴＰ, １２ｕｇ ＲＮＡ,２５ ｐｍｏｌ引物,并在此条件下测得 ＦＤ－ＴＲＴ和 Ｔａｑ ＤＮＡ 聚合酶逆转录活力与聚合酶活力的相对比值分别为０．０５６,０．００４５．