An enzyme immunoassay for mouse epidermal growth factor utilizing a liquid phase double-antibody system

Summary An enzyme immunoassay for mouse epidermal growth factor (EGF) involving a liquid phase double-antibody system was developed. The EGF--galactosidase conjugate prepared was stable for at least 8 months. By this method, EGF was detectable at a concentration as low as 20 pg per tube. The concentrations of EGF in various tissues of mice are also presented.This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.     

结构方程模型在兴安落叶松林生长中的应用

【目的】通过结构方程模型,确定气候、土壤和海拔对兴安落叶松林生长指标的影响以及路径关系。【方法】选取年平均气温、年平均降水量、太阳辐射、土壤全氮含量、土壤有机碳密度和海拔作为影响因素,探究兴安落叶松地上生物量、地下生物量、树高与这些影响因素的关系,并利用AMOS 21.0软件构建衡量兴安落叶松生长的3个指标与气候、土壤和海拔的结构方程模型。【结果】兴安落叶松的地上生物量、地下生物量随着海拔、年平均降水量的增大呈现先增大后减少的趋势,树高随着海拔的增加而增加。地上和地下生物量随着土壤有机碳密度的增加而增加。海拔对兴安落叶松生长的总效应系数为0.200且是正向效应,海拔对兴安落叶松生长的直接效应(0.224)大于间接效应(-0.024);气候因子对兴安落叶松林生长的总影响系数为-0.771且是负向效应;土壤因子对兴安落叶松生长的总影响系数为-0.216,其对兴安落叶松林生长起到一定的抑制作用。【结论】根据结构方程模型的路径系数,气候因子的总影响系数绝对值最大,其次是土壤和海拔,兴安落叶松林静态生长主要受到气候因子的制约。     

墩高对不对称高墩连续刚构桥力学特性的影响（英文）

对不同墩高下的不对称高墩连续刚构桥成桥阶段进行了力学特性研究,获得了高墩连续刚构桥在不同墩高下的主梁和桥墩的内力、位移变形特性.研究结果表明,桥墩墩高的变化对主梁内力、主梁竖向位移、墩内力、墩横向位移均产生不同程度的影响,其中对跨中主梁竖向位移和墩顶剪力的影响较大,主梁竖向位移随着墩高的增大而增大,最大位移处平均增幅14.3%,墩顶主梁剪力随着墩高的增大而减小,平均变化率37.1%.     

含铝高速钢中奥氏体非均匀长大的机制

M2AI和M2高速钢经一次和二次淬火后,奥氏体晶粒和未溶碳化物的定量研究表明,在高温r/f比值相同的条件下,由于淬火前原始组织的差别,可发生正常(均匀)和异常(非均匀)两类长大。根据异常粗晶形成初期的组织观察,提出了“连续-非连续”再结晶模式和“合并—推移”式的生长机制。提出了减轻或消除M2AI钢混晶的工艺原则。     

几种巴西橡胶三合树的生长状况和光合强度

不同组合巴西橡胶三合树在福建南部的生态条件下,其生长强度,生理代谢和抗逆能力都存在差异。本文中主要研究比较了几种橡胶三合树的生长强度,光合作用强度及其变化。研究结果表明,三合树的生长强度和光合强度明显比对照树高,不同组合三合树的生长强度和光合强度与它们的抗寒力成正相关。     

Inhibition of the growth of cariogenic bacteria in vitro by plant falvanones

Phytoalexins, defensive compounds produced by plants against microbial infections, were purified fromSophora exigua (Leguminosae) and their growth inhibitory effects on oral cariogenic bacteria were determined in vitro. Among three isolated compounds, 5,7,2,4-tetrahydroxy-8-lavandulylflavanone completely inhibited the growth of oral bacteria including primary cariogenic mutans streptococci, other oral streptococci, actinomycetes, and lactobacilli, at concentrations of 1.56 to 6.25 g/ml.     

Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells^1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently.     

Thrombospondin co-localises with TGFβ and IGF-I in the extracellular matrix of human osteoblast-like cells and is modulated by 17β estradiol

Thrombospondin (TSP) is a multifunctional glycoprotein which is synthesised by several cell types including osteoblasts, and incorporated into the extracellular matrix (ECM) of these cells. The function and regulation of TSP in bone is not clear. In this study, using a long term culture model of human osteoblast-like cells, we examined the distribution of TSP in the ECM and its modulation by added estradiol. In this model the osteoblast-like cells form a regular multilayer which continues to increase in depth up to 50 days post confluence. In the ECM of these cultures and in 19-week fetal bone, the bone markers osteocalcin and alkaline phosphatase were diffusely distributed in the matrix. In contrast, labelling for TSP was concentrated, confined to the banded collagen and its immediately adjacent ECM. This pattern of labelling resembled that of the growth factors transforming growth factor-I (TGF), and insulin-like growth factor-I (IGF-I), with which TSP label co-localised. Labelling intensities were comparable between fetal bone and the in vitro material for TSP, TGF and IGF-I. TSP label was present by 10 days post confluence, reached a maximum by 20 days, and declined slowly thereafter, a time course which was similar to that of IGF-I. Incubation of osteoblast-like cell cultures with 17 estradiol resulted in an increase in multilayer depth and a maximal 3-fold increase in TSP labeling at 30 days as well as approximately 2-fold increases for TGF and IGF-I. The dose-response relationship for these responses to estradiol treatment was biphasic with maximal increases at 10^–10 M–10^–11 M of added estradiol. Treatment with 17 estradiol produced labelling intensities that were not significantly different from controls. Studies with other cell types have suggested that TSP may be involved in modulation of growth factor activity. The similarities between TSP, TGF and IGF-I, in terms of their distribution and regulation by 17 estradiol treatment, may indicate a role for TSP in modulating bone cell proliferation and function through interaction with local growth factors.     

战斗机网络攻击指令引导控制方法

对战斗机网络攻击指令引导控制方法进行了研究。建立了导弹拦射攻击模型和导弹发射边界模型;以航向角和高度为引导指令建立网络指令引导解算模型;基于典型作战场景对上述模型进行仿真实验分析。仿真结果表明:不管针对非机动目标还是机动目标,采用网络攻击指令引导方法都能实现最大火力包线的战斗机网络攻击引导。     

桂东南马尾松工程幼林生长量及其生物量节律研究

１９９１年～１９９６年,对桂东南马尾松工程幼林的生长量及生物量进行连续的定位定期观测。结果表明：马尾松工程林６年生幼林的林木蓄积量和生物量分别为３６．１６ｍ３／ｈｍ２和３６．８６ｔ／ｈｍ２,分别比常规造林１１年生和不施肥同龄林增加３倍～６倍;揭示了马尾松幼林生长量规律及其影响的主要气象因子,马尾松工程幼林各器官生物量随年龄的变化规律,并分别建立与地径逐月生长量的回归预测模型。