人脐带静脉血管内皮细胞的体外培养及其凋亡的研究

本文从提取生长因子入手，建立人脐带静脉血管内皮细胞系，用电镜进行了细胞鉴定，然后用去除生长因子（FGF和胎牛血清）的方法诱导细胞凋亡，利用荧光显微技术、DNA凝胶电泳、电镜技术和流式细胞分光光度技术，研究了血管内皮细胞凋亡过程中超微结构和细胞周期的变化。发现去除生长因子3h后，在细胞发生明显的DNA片段化和形成凋亡小体的同时，细胞核与细胞质的超微结构发生了明显的变化，S期细胞显著减少，G1期无明显变化，G2细胞显著增多，说明用去除生长因子的方法诱导血管内皮细胞凋亡时，细胞从G2期脱离细胞周期进入凋亡程序，本文的人血管内皮细胞在体外的成功培养和对该细胞凋记的研究对深入研究其凋亡的分子调控机制具有重要的参考价值。     

伊贝母细胞悬浮培养的研究

研究了伊贝母细胞悬浮培养过程中接种量、培养方式和激素浓度对细胞生长的影响；测得细胞生长周期为１９～２４ｄ；分析了培养液的ｐＨ、蔗糖浓度、电导率和Ｄｏ变化的原因。鉴定了悬浮培养细胞中含有生物碱，其总生物碱和西贝素含量均高于栽培鳞茎。     

玄武湖水、金川河水诱发的蚕豆根尖细胞微核

玄武湖、金川河分别是南京著名的风景地和重要的环城河流，多年来玄武湖、金川河的水质污染越来越引起人们的重视．南京市内不少工厂的工业废水及居民的大量生活污水流入其中，特别是有毒物质，包括酚、苯、汞、氰化物、直链烷基苯磺酸钠、有机磷等．这些物质对人体健康的远期效应倍受人们关注．为此对玄武湖水、金川河水引起蚕豆根尖细胞染色体损伤所产生微核的情况进行了研究，就二处水样对生物体的致突变性及对人体健康的潜在危害进行了讨论     

相变存储器多态存储方法

提出两种实现相变存储器多值存储的方法.这两种方法基于新的机理,即通过存储单元结构或相变材料的改进来得到在传统"高阻态"和"低阻态"两态之间稳定的中间电阻状态.与传统实现相变多态存储的方法相比,利用这两种方法实现的多值相变存储,可以有效地提高多态存储的可靠性和抗噪声性能,有利于在提高存储密度的同时简化外围电路的设计,在实际应用中有良好的前景.     

网格环境中资源发现与监测机制研究

网格资源发现与监测是网格计算的一个重要组成部件,为了对网格资源进行调度和分配,网格系统必须对动态的网格资源进行实时、准确地监测,以充分了解资源的当前状态。通过对网格资源发现与监测标准的分析,结合Agents的跨平台通讯和智能化的特性,给出了一种基于Agents的分层次的网格资源发现与监测模型,该模型以较小的系统代价,可以获取较好的系统性能。     

Synthesis of Ta C and Ta_2C from tantalum and graphite in the laser-heated diamond anvil cell

The pressure dependence of the onset of the formation of Ta C and Ta2 C from the elements has been investigated by in situ X-ray diffraction and pyrometry.Ta C has been synthesized by the reaction of Ta and graphite at pressures between 8.6 and 14.3 GPa and at temperatures up to 2,300 K using a laser-heated diamond anvil cell. The products were characterized by X-ray diffraction. Ta and graphite begin to react around 1,100 K at ambient pressure conditions, and the reaction temperature increases with increasing pressure. A linear extrapolation of these data is consistent with recent observations of the formation of Ta C at 90 GPa and 3,600 K. We show that diffusion of carbon into tantalum significantly changes the lattice parameter of up to 2 % in the pressure range of up to19 GPa. In some experiments, Ta2 C was formed concomitantly. The experimentally determined bulk modulus of Ta2 C is B0;exp：= 286（5） GPa. Other tantalum carbide phases were not observed.     

Behavior of MC3T3-E1 Osteoblast Cultured on Chitosan Modified with Polyvinylpyrrolidone

The physical and chemical properties of four kinds of modified chitosan materials made by blending chitosan with polyvinylpyrrolidone （PVP） were investigated. All four of these modified chitosan materials were hydrophilic with water contact angles ranging from 59°to 69°. Fourier transform-infrared spectra of the modified materials showed a new band at 1288 cm^-1, implying formation of a surface physical interpenetrating network structure. Enzyme linked immunosorbent assay results indicated that much less fibronectin was adsorbed on the modified materials than on only chitosan. The viability of MC3T3-E1 osteoblasts cultured on the materials was assessed by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl- 2H-tetrazolium bromide assay. The results show that adding PVP10000 into the chitosan promotes adhesion of MC3T3-E1 osteoblasts on the modified materials, but has no effect on cell growth and proliferation; while adding PVP40000 reduces cell adhesion, growth, and proliferation. The results suggest that the increased hydrophilicity of the material surface does not always improve its biocompatibility, which will influence the selection and design of biomaterials.     

非晶硅太阳电池在反偏压下的光照特性

研究了温度循环和在反偏压下强光照对非晶硅太阳电池性能的影响。电池在90℃,4.5V反偏压下,经550mW/cm~2 的卤钨灯光照后,短路电流增加.光致衰降减小,长波响应改善.用内电场的变化进行了解释.     

GFP标记的GDNF重组腺病毒载体的构建和体内外表达

通过构建绿色荧光蛋白(GFP)和胶质细胞源性神经营养因子(GDNF)共表达的腺病毒载体,讨论其在治疗脊髓和脑有关神经系统疾病的潜在应用价值,了解腺病毒载体在大鼠体内的分布及在体外分泌外源基因产物能力,并对其生物安全性作了初步考察,为GDNF重组腺病毒载体在临床和基础研究中的应用作了一定准备．     

三维网格的生成

采用轴向拉伸法生成三维三棱柱网格．首先生成二维网格基面，选取三维场域的最复杂截面进行二维剖分，然后再将三维网格拉伸成三维网格．并画出了三维网格生成的流程图．