珠江三角洲网河潮汐空间特征

珠江三角洲网河区径潮作用复杂，其潮汐的特征明显受到径流、地形等影响。本文根据1999年洪季和2001年枯季的实测逐时潮位资料，应用数理统计、作图、潮汐调和分析等方法来分析珠江三角洲网河的潮汐空间特征，包括洪、枯季平均潮位的空间变化，潮汐调和常数（分潮振幅和迟角）的空间分布特征及沿程变化。同时，这些潮汐特征也反映了潮波在传播过程中受到径流、地形和边界条件作用后能量和周期的变化。     

CD40L对B淋巴细胞表面分子及分泌IL-12的影响

在体外使用CD40L作用于B淋巴细胞使之活化,通过测定B淋巴细胞表面分子及分泌细胞因子的变化,探讨B淋巴细胞作为抗原递呈细胞在抗肿瘤免疫中的优势,为B淋巴细胞特异性活化细胞毒T淋巴细胞(cytotoxic T lymphocyte,CTL)进而发挥其肿瘤细胞杀伤效应奠定基础.取7名健康成人外周静脉血,经淋巴细胞分离液分离人外周血单个核细胞(PBMC),分为对照组和实验组,实验组培养液中加入加入CD40L和IL-4,共培养21 d.观察B淋巴细胞生长状况,并在第7 d、第14 d和第21 d,用流式细胞仪(FCAS)测定其表面分子CD80、CD86和CD19,在第21 d,使用酶联免疫吸附法(ELISA)测定培养液中的IL-12水平.结果显示,在CD40L的作用下,B淋巴细胞呈克隆样增殖,并高表达表面分子CD80/CD86和CD19,和对照组相比,B淋巴细胞分泌IL-12水平升高(P＜0.05).上述实验结果说明通过CD40L的刺激,B淋巴细胞得到了活化,提示通过该途径活化的B淋巴细胞具备了作为抗原递呈细胞而发挥其抗肿瘤免疫治疗的能力.     

西沙蒸发量的时间变化特征及与日照时间和近地面风速的关系

用中国西沙站1962-2004年月蒸发量、日照时间和近地面风速资料,分析了西沙各季节蒸发量的时间变化特征及其与日照时间、近地面风速变化的关系。结果表明:西沙春、夏和冬季蒸发量以年代际变化方差占优,年际变化方差次之;秋季蒸发量的年际变化方差和年代际变化方差相当。四季蒸发量年代际变化与近地面风速、日照时数的年代际变化都有显著的正相关。而蒸发量的年际变化仅与日照时数的年际变化有显著的正相关。四季蒸发量减弱的变化趋势是由近地面风速的减弱和太阳辐射的减少造成的。     

对称化L2-偏差下组合设计的均匀性

基于对称化L2-偏差讨论了组合设计的均匀性,在两种特殊的情形下分别得到了两水平部分因子设计的组合设计的离散偏差的下界,数值例子说明这些下界是紧的,因此这些下界可以作为寻找最优折叠反转方案的基准。     

Geographical variation in the golden‐striped salamander,Chioglossa lusitanica Bocage, 1864 and the description of a newly recognized subspecies

Morphological and genetic data for the Iberian golden‐striped salamander, Chioglossa lusitanica, demonstrate the existence of two groups with southern and northern ranges, connected by a zone of intergradation in central Portugal. Because reproductive isolation between them is incomplete we consider the groups to be subspecies. The type locality of C. lusitanica (Buçaco near Lousã) is situated inside the mixed zone. This necessitates identification of the nominotypical subspecies. We sequenced a fragment of mitochondrial DNA from one of the species' syntypes and we determined what position over a latitudinal transect maximizes the morphological discrimination between the groups. Both approaches indicate that C. lusitanica from Buçaco represents the southern subspecies. A new subspecies of C. lusitanica is described from a northern locality (Valongo near Porto in north‐western Portugal). A lectotype is designated for Chioglossa lusitanica.     

Genomic and genic sequence variation in synthetic hexaploid wheat （AABBDD） as compared to their parental species

In order to understand the genomic changes during the evolution of hexaploid wheat, two sets of synthetic hexaploid wheat from hybridization between maternal tetraploid wheat （AABB） and paternal diploid goat grass （DD） were used for DNA-AFLP and single strand conformation polymorphism （SSCP） analysis to determine the genomic and genic variation in the synthetic hexaploid wheat. Results indicated that more DNA sequences from paternal diploid species were eliminated in the synthetic hexaploid wheat than from maternal tetraploid wheat, suggesting that genome from parental species of lower ploidity tends to be eliminated preferentially. However, sequence variation detected by SSCP procedure was much lower than those detected by DNA-AFLP, which indicated that much less variation in the genic regions occurred in the synthetic hexaploid wheat, and sequence variations detected by DNA-AFLP could be derived mostly from non-coding regions and repetitive sequences. Our results also indicated that sequence variation in 4 genes can be detected in hybrid F1, which suggested that this type of sequence variation could be resulted from distant hybridization. It was interesting to note that 3 out of the 4 genes were mapped and clustered on the long arm of chromosome 2D, which indicated that variation in genic sequences in synthetic hexaploid wheat might not be a randomized process.     

铝电解多功能机组变幅四连杆打壳机构设计改进

铝电解多功能机组是大型铝电解生产的机械化专业操作设备,打壳机构是铝电解多功能机组中的主要工具之一,结构非常复杂。本文通过对旧式连杆打壳机构在实际应用中产生问题的分析及缺陷的设计改进,重点介绍了一种结构相对简单、工作效率高、可将电解槽中缝的氧化铝结壳打碎的新型变幅四连杆打壳结构,经过实践运用,效果良好,是一种极具推广价值的新型结构。     

一维非线性奇异边值问题的有限元方法

使用对称有限元方法，研究了奇异非线性两点边值问题有限元解的收敛性，给出了最佳阶的误差估计     

银杏的有效成份及提取工艺

本文综述了银杏中的提取成份黄酮类、萜内酯、酸类、氨基酸、微量元素等，并介绍了它们目前的提取工艺。     

风信子的组织培养和植株再生

以风信子鳞茎为外植休外灭菌后切成小块接入到风信子愈伤组织诱导培养基MS1上得到了愈伤组织，这些愈伤组织在含6BA，KT的MS分化培养基（MS2）上分化得到了牙，并在同样的培养基上有少量的根分化出来，当牙移至生根培养基上时可生成大量健壮的根，从而得到完整植株，建立了风信子离体诱导再生植株的体系，使用的方法不需要进行低温处理，摆脱了因设备不足在组织培养生产中的限制，同时还对风信子的灭菌方法进行了研究，发现对风信子地下器官的表面灭菌主应以75％乙醇浸泡5min后再以15％新洁尔灭浸泡10min，剥叶用0．1％，升汞浸泡15min，无菌水清洗5次效果最好。