Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

Research advances in gene therapy for Parkinson's disease

During the long period of time when people have been seeking for an effective therapeutic method for PD, the gene therapy has shown greater and greater advantages over other methods. It can be performed mainly in two ways: ex vivo and in vivo. With the former, TH gene as well as some neurotrophic factor genes (such as GDNF, BNDF genes) can be invited to some cell lines or primary cells thus forming engineered cells and then implanting them into brain. While with the latter, viral vectors including HSV-1, Ad, AAV that can be utilized to construct recombinant viruses, or non-virus vectors can be used to delived DNA into brain directly. The present review summarizes the recent research advances in the gene therapy for PD, and it is reasonable for us to predict a notable progress in prevention and treatment for PD in the next decade.     

用于数据挖掘的粗集产生多重知识库的方法

提出了一种用于数据挖掘的粗集产生多重知识库的方法．该方法基于粗集理论的简化和决策矩阵．首先从知识表示系统中消除冗余属性,产生简化表,形成简化的知识表示系统,提高学习的有效性和精确性．然后从简化表中推导出决策矩阵,通过决策矩阵获取最小决策规则,计算包括所有必需属性的简化集合,给出计算最小决策规则和计算多重简化的算法．最后由相应的简化对最小决策规则进行分组形成多重知识库,给出产生多重知识库的算法．     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)     

提高注射法导入外源基因棉铃成铃率的技术研究

对采用注射法导入外源基因的棉铃,在激素种类、不同生态条件、结铃部位、结铃性、整枝方式等方面进行分析,探讨了导入棉铃落铃机理和提高导入棉铃成铃率的技术途径。     

利用初等行变换求极大无关组的一些条件

本文讨论利用初等行变换求行向量组的极大线性无关组的方法,澄清一些线性代数教学用书中存在的一种模糊认识,并给出修正后的方法。     

外源基因（bar）在转基因燕麦（AvenasativaL.）后代中的遗传与表达

通过微弹轰击获得的含有ｂａｒ基因的转基因燕麦植株自交产生转基因后代。利用ＰＣＲ方法检测ｂａｒ基因在后代中的传递情况。     

灵长类酪氨酸酶基因外显子1序列进化研究

酪氨酸酶是黑色素合成当中的关键酶,酪氨酸酶基因的突变将导致白化病,测定了黑猩猩,倭黑猩猩,大猩猩、猩猩、长臂猿、食蟹猴、猴猴９个灵长类中代表物种的酪氨酸酶基因外显子１的ＤＮＡ序列。     

大肠杆菌编码区5′端碱基的统计分析

统计了大肠杆菌１２８８个编码序列开始的３３个碱基位点（１１个密码子）上各碱基出现的概率,发现第２、第３密码子各位点上的碱基概率分布与其它密码子上的碱基分布概率不一样;碱基Ｇ和Ｔ出现的概率从第４个密码子后呈现明显的３周期分布．我们还研究了这些位点上的碱基概率分布与基因表达水平的关系,发现高表达基因和低表达基因在第２、第３密码子各位点上,其各种碱基的概率分布是有区别的;高表达基因碱基Ｇ和Ｔ的概率分布３周期性更明显．