企业技术创新的关键在于企业与科技人员互动

企业技术创新要求企业与科技人员合作 ,合作的基础是利益诱导。在分析了企业与科技人员的利益目标差异的基础上 ,用一个抽象的组合激励理论模型 ,说明了两者“双赢”的行为选择 ,为推动企业技术创新提供了一种思路。     

Cloning and functional analysis of chloroplast division gene NtFtsZ2-1 in Nicotiana tabacum

FtsZ protein plays an important role in the division of chloroplasts. With the finding and functional analysis of higher plant FtsZ proteins, people have deepened the understanding in the molecular mechanism of chloroplast division. Multiple ftsZ genes are diversified into two families in higher plants, ftsZ1 and ftsZ2. On the basis of the research on ftsZ1 family, we analyzed the function of NtFtsZ2-1 gene in Nicotiana tabacum. Microscopic analysis of the sense and antisense NtFtsZ2-1 transgenic tobacco plants revealed that the chloroplasts were abnormal in size and also in number when compared with wild-type tobacco chloroplasts. Our investigations confirmed that the NtFtsZ2-1 gene is involved in plant chloroplast division.     

Recent progress in polymer-based gene delivery vectors

The gene delivery system is one of the three components of a gene medicine, which is the bottle neck of current gene therapy. Nonviral vectors offer advantages over the viral system of safety, ease of manufacturing, etc. As important nonviral vectors, polymer gene delivery systems have gained increasing attention and have begun to show increasing promising. In this review, the fundamental and recent progress of polymer-based gene delivery vectors is reviewed.     

Expression of human clotting factor Ⅸ mediated by recom- binant lentiviral vector in cultured cells and hemophilia B mice

To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driven by ubiquitin-C promoter, FUXW, and by ABP liver specific promoter, FAXW, was constructed respectively. Recombinant lentivirus was harvested from 293T cells by calcium phosphate-mediated transient cotransfection of three plasmids (transgene vector, CMV腞8.2, VSV-G). hFⅨ expression was detected in supernatant of 293T, BHK and L-02 cells infected with FUXW virus, whereas higher expression of hFⅨ levels (630 ng/106 cells/48 h) was detected only in L-02 cells infected with FAXW virus. Serum hFⅨ antigen was detected in all hemophilia B mice treated with FAXW virus by tail vein injection, an efficiency level of hFⅨ was observed (45 ng/mL, approximately 1% of normal human levels), the expression lasted for more than 60 d. The results indicated that HIV-based lentiviral vectors offer a promising approach to the gene therapy of hemophilia B.     

植物功能基因组研究进展

基因组学研究已开始从结构基因组学转向功能基因组学。对功能基因组学研究的重要意义及内容、方法进行了综述，并对植物功能基因的研究进展及应用前景作了介绍。     

文本分类中的特征选取算法

分析了常用的几种特征选取方法，提出了改进互信息算法。实验结果显示改进的互信息算法是可行的。     

鄂尔多斯蒙古族、汉族5项遗传学指标研究

调查了内蒙古鄂尔多斯蒙古族(508例)、汉族(461例)人的5项头面部形态特征.5项指标是鼻梁侧面观、鼻孔形状、下颏突出度、额头发际、头发形状.调查显示:(1)这两个人群多为非凸型鼻梁、宽型鼻孔、非突型下颏、直发,额头发际有尖率与无尖率接近.(2)在内蒙古诸人群中,这两个人群凸鼻梁率低、宽型鼻孔率高、突型下颏率低、额头发际有尖率高、直发率低.(3)5项指标彼此多不相关,仅鼻梁类型与鼻孔形状相关,鼻梁类型与下颏类型相关.     

一种用于多峰函数优化的改进混合遗传算法

针对遗传算法在处理复杂多峰函数优化问题中存在的一些缺陷，提出了一种改进型的混合遗传算法，它在小生境技术的基础上引入单纯搜索算法、最优保存算法和近优淘汰算法，并使之相互结合，经编程实践证明，这种改进的混合遗传算法在处理复杂多峰函数优化问题时，局部搜索能力和克服过早收敛能力方面都显著优于标准遗传算法，并在实际应用中取得了较好的效果。     

试论达尔文进化论在生态学发展中的地位

介绍了现代生态学发展的特点和达尔文的进化论及完善过程，认为达尔文提出的生态思想是朴素的生态观，其进化论是种—种群的进化，从本质上并未涉及到其他生态学等级的进化；必须以普遍联系的系统观和时空观评价生态学发展现状，生态学研究也必须注重局部各因子之间、整体与局部之间的作用关系。初步探讨了能否象牛顿力学那样将自然选择力作为推动生物进化的“万有引力”的生态学研究问题。     

数据仓库中视图选择算法的分析与比较

数据仓库中的视图选择关系到数据仓库的效率和维护代价，是数据仓库中研究的重要问题。本就已有的视图选择算法加以分析、比较，以此描述视图选择算法的研究现状和发展方向。