Frequent mtDNA mutations and its role in gastric carcinogenesis

In order to disclose the relationship between mutations of mitochondrial DNA (mtDNA) and gastric carcinogenesis, we screened the entire mtDNA sequence in 30 cases of human gastric cancer and matched normal tissues by using denaturing high-performance liquid chromatography (DHPLC) and DNA sequencing. Our data showed that high frequency (66.7%, 20/30) of mitochondrial genome mutation occurred in gastric cancer. Among these variants, 17 cases (56.7%, 17/30) were identified to be somatic mutation. High level mutant frequency was found in ND4, ND5 coding genes and D-loop control region, which was 36.7%, 26.7% and 30% respectively. Comparing with complexes Ⅲ, Ⅳ and Ⅴof the electron transport chain, we found that variants appeared to be more frequent in the subunit genes of complexⅠ. Most of mutations were base substitutions (85.4%, 41/48). Our results suggested that mutations of subunit genes encoding complexⅠ, especially ND3, ND4 and ND5 genes, might contribute to human gastric carcinogenesis.     

基于DSP的变频调速控制系统设计

应用矢量控制的原理，以DSP为核心，开发出一套基于DSP的变频调速系统。对整个系统的硬件电路和驱动软件实现作了详细的介绍，实现对异步电机的控制，提高了能量的利用效率。     

经济学视角下新闻保护调整模式析论

政府直接管制与基于市场的经济手段在新闻公共政策的调整领域都有其有利的地位,新闻政策的关键在于寻找市场调节与政府直接干预的均衡点,以达到新闻保护与经济发展协调的目的。从外部性、公共产品、信息不对称三个方面从"市场失灵"的角度分析新闻保护问题的法律调整模式,解决新闻领域的问题需要"看的见的手"和"看不见的手"兼而有之。     

提升普通高校学报影响力的措施和方法

分析了普通高校学报影响因子低、影响力小的原因,重点论述了提高普通高校学报被引频次、影响因子和影响力的措施和方法.     

加工硬质合金端铣刀刀槽时的工艺调整计算

硬质合金端铣刀刀体上的刀槽，通常将设计为槽底与铣刀轴线倾斜δ角，槽侧壁与铣刀轴线倾角为ω且偏移一个距离为ｅ，以使刀齿装夹在刀体上后获得端铣刀所需的几何角度。本文主要论述在万能铣床上铣削铣刀体上具有δ、ω、ｅ的刀槽时必要的工艺调整，利用矢量及座标转换推导了加工刀槽时必要的参数计算公式，为高效率、高质量地进行铣刀体刀槽的加工提供了一种精确可行的参数计算方法。     

细菌代谢调控机制的系统性：初论从分子水平理解细菌代谢调节的系统性

利用系统科学的基本原理,从分子水平上对细菌代谢调节,功能网络及其与外界环境的关系进行了初步探讨,得出了细菌代谢调节系统性的一般结论.     

Pathways for oxidative fuel provision to working muscles: Ecological consequences of maximal supply limitations

The study of metabolic fuel provision and its regulation has reached an exciting stage where specific molecular events can be correlated with parameters of the organism's ecology. This paper examines substrate supply pathways from storage sites to locomotory muscle mitochondria and discusses ecological implications of the limits for maximal flux through these pathways. The relative importance of the different oxidative fuels is shown to depend on aerobic capacity. Very aerobic, endurance-adapted animals such as long distance migrants favor the use of lipids and intramuscular fuels over carbohydrates and circulatory fuels. The hypothesis of functional co-adaptation between oxygen and metabolic fuel supply systems allows us to predict that the capacity of several biochemical processes should be scaled with maximal oxygen consumption. Key enzymes, transmembrane transporter proteins, glucose precursor supply and soluble fatty acid transport proteins must all be geared to support higher maximal glucose and fatty acid fluxes in aerobic than in sedentary species.     

基因研究中的知情权探析

20世纪以来，基因研究得到了广泛重视，尤其是人类基因组序列的测定给制药、保健、农业和食品制造等产业带来了巨大商机。与此同时，基因侵权事件屡屡发生，尤其是在发展中国家。目前在中国，出现的最为普遍、最为严重的侵权行为就是侵犯被研究对象的知情权。对此，应加强知情权的保护，严格约束中方合作单位，加侠基因方面的立法，加强公民的自我保护意识。     

生命物理学研究的新突破

简要介绍了生命科学研究的新突破,包括人类嗅觉谜团被解开,激活自愈机能治疗肝炎,运用基因重组或剔除基因治疗癌症获重大进展等.