晚期胃癌中的肿瘤异质性表达与侵袭转移的相关性研究

应用免疫组化和突变分析方法,分析晚期胃癌原发灶和转移灶nm23,S100A4和p53的表达差异,探讨肿瘤内部异质性在胃癌转移发生中的意义。结果发现转移抑制基因nm23在转移灶中的表达显著上调,转移促进基因S100A4在转移灶中的表达显著下调,癌基因p53在两者中的表达和突变没有明显差异。nm23在原发瘤中的表达下调以及S100A4和p53的过度表达与胃癌的高侵袭性显著相关。数据显示:nm23和S100A4在胃癌的转移中发挥作用,而p53是胃癌发生的一个晚期事件。     

The clinical practice of 252Cf neutron brachytherapy devices on malignant tumor in China

The 252 Cf neutron brachytherapy devices were designed and manufactured after solving those problems： the shield difficulties for 252Cf neutron; the method and facilities for 252Cf neutron source deliver; the dose calculation and measurement for 252Cf neutron. From 1999 to 2009, more than twenty 252Cf neutron brachytherapy devices had been installed in China. The Chinese radiation oncologists implemented the 252 Cf neutron braehytherapy on more than 5 000 different kinds of cancer patients, including cervix cancer; endometrium cancer and achieved ideal results. For special presentation, they had breakthrough on radical treatment for low lying rectal adenocarcinoma with sphincter preservation and melanoma patients after the modification of 252Cf neutron intracavitary applicator and dose calibration.     

超氧化物歧化酶在模拟人体胃肠环境中稳定性的研究

本文以中国药典中的人工胃液和人工肠液作为模拟人体胃肠环境，对超氧化物歧化酶的稳定性进行了一系列单独及综合的研究。研究结果发现，超氧化物歧化酶在人工胃液中会受到较强的破坏，而在人工液中则颇为稳定。同时还发现，尽管是天然结构的超氧化物歧化酶对胃蛋白酶和胰酶具有较强的耐受力，但被胃酸处理后结构松散的超氧化物歧化酶却可被胃蛋白酶和胰酶较有效地水解。     

残胃236例临床内镜分析

本文报告全部经胃镜和病理检查证实的手术后胃２３６例．ＢｉｌｌｒｏｔｈⅠ式残胃（Ｂ－Ⅱ式）２４例，ＢｉｌｌｒｏｔｈⅡ式残胃（Ｂ－Ⅱ式）２１２侧．结果显示吻合口炎及残胃炎发生率为最多；病理以慢性炎症为最多，其次为不典型增生病变．检出残胃癌１２例（其中手术原因不明者３例）．残胃复发癌１２例．并对残胃癌、术式等问题进行讨论．认为术前术式选择宜从严掌握．术后必须内镜随访，尤其对胃癌术后、残胃癌前病变应定期胃镜及病理检查．     

蟾蜍离体胃电和胃内压的关系

实验选用100只蟾蜍,观察离体胃正常的电活动和压力变化,试图从结构差异和进化历史等方面,用比较的方法,初步探讨蟾蜍离体胃电和胃内压的特征及其相互关系,以求认识胃肠道机能的基本特性,为胃肠道比较生理学提供参考资料.     

乙酰胆碱对人胃粘膜上皮细胞和胃腺癌细胞内钠、磷浓度的影响

采用OlympusAu640生化分析仪,检测了乙酰胆碱作用后培养的人胃粘膜上皮细胞和胃腺癌细胞内钠和磷的含量,以探讨乙酰胆碱对人胃粘膜上皮细胞和胃腺癌细胞内钠、磷浓度的影响。结果发现:与正常胃粘膜上皮细胞比较,胃腺癌细胞内钠浓度偏高(P<0 001),而磷浓度降低(P<0 01)。在乙酰胆碱作用下,胃腺癌细胞内钠和磷浓度均升高(P<0 05);正常胃粘膜细胞仅有钠浓度升高(P<0 001),磷浓度无变化。阿托品能阻断乙酰胆碱对磷浓度的升高作用(P<0 05),但对钠浓度无影响。     

中国美利奴新疆军垦型羊反刍时的前胃电活动

反刍时前胃肌电活动规律表明:绵羊前三胃之间存在生物电发生的相关性。反刍的发生与网胃附加峰电簇直接相关;瘤胃峰电出现时相上的提前,从瓣胃峰电的改变揭示出瓣胃活动也与反刍有关。     

Phospholipid flippase associates with cisplatin resistance in plasma membrane of lung adenocarcinoma A549 cells

The fusion of the liposomes containing N-(7-nitro-2, 1, 3-benzoxadiazol-4-yl)-i ,2-hexadecanoylSn-glycero-3-1abeled phosphatidylethanolamine (NBD-PE) with A549 and A549/DDP cells was performed, and the activity of the phospholipid flippase in the plasma membrane of the cells was measured by fluorescence intensity change of NBDPE in the outer membrane. When A549 or A549/DDP cells containing N BD-PE were incubated at 37 C for 0, 30, 60 and 90 min, the fluorescence intensities in the outer membrane of the cells were 0%, 1.4%, 2.9% and 7.8% for A59cells, and 0%, 10.5 %, 15. 5 % and 18.3 % for A549/DDP cells respectively, demonstrating that the phospholipid flippase was distributed in the plasma membrane of As49 cells, but its activity in the drug-resistant A549/DDP cells was much higher than that in the A549 cells. When the A549/DDP cells were incubated with a multidrug resistance reverse agent, verapamil, for 60 min at 37C, the results showed that the NBD-PE in outer membrane decreased by 25.0% compared with the control's. Furthermore, when A549/DDP cells were incubated with 25 μmol/L cisplatin, which is a specific anticancer drug, the flippase activity decreased by 31.6%, and it further decreased with the increase of cisplatin concentration, suggesting that phospholipid flippase in the membrane might be related to the cisplatin-resistance of human lung adenocarcinoma cancer cells.     

胃癌中p53基因失活及其蛋白产物的免疫组化分析

应用ＰＣＲ－ＳＳＣＰ银染，Ｓｏｕｔｈｅｒｎ杂我及免疫组化等方法同步研究２５例胃癌标本的ｐ５３基因突变，杂合性丢失及蛋白持表达，在２２例胃癌中同时获得有关ｐ５３基因突变和杂合性丢失的检测结果，被检出ｐ５３基因突变的５例胃癌中，２例伴有杂合性丢失，３例仅有ｐ５３基因突变。     

COC1/DDP亚细胞中波形纤维蛋白的肽质谱指纹分析

目的寻找与鉴定卵巢癌耐药株中的差异表达蛋白质。方法应用高分辨二维凝胶电泳分离卵巢癌细胞中差异表达的波形纤维蛋白,并通过基质辅助激光解吸电离飞行时间质谱对其进行分析与鉴定。结果获得了分辨率和重复性均较佳的凝胶电泳图谱,图像分析显示波形纤维蛋白表达明显下调。结论与其他方法相比,应用凝胶电泳和生物质谱联用技术分析与鉴定未知蛋白具有简单快捷的特点。