列车超速防护系统中主机自诊断方法

分析了 CPU,ROM,RAM 的几种故障检测方法,针对列车超速防护系统主机工作的特点,实现了系统开机时完备的主机功能级自诊断(包括关键 I/O 口)和工作中短时问的周期自检.利用主机中双 CPU 工作的特点,通过双口 RAM 通道,完成双机互检.能监视程序执行行为特征,发现单 CPU 故障或程序跑飞,并对 CPU 的执行结果进行合理性检测,提高了整个系统的故障检测覆盖率.还对故障检测覆盖平及检测代价做了评估.     

拟加权Bergman空间上线性泛函表示

本文定义了拟加权Bergman空间A_ф~(p.a),当P<1时,我们得到了A_ф~(p.a)有界线性泛函表示.     

两类含多个未知函数的函数方程的可微解

讨论了两类含多个未知函数的函数方程可微解的存在性条件,并将其求解问题归结为常微分方程的求解问题。     

n阶泛函微分方程的非振动解

给出了一类n阶泛函微分方程的有界非振动解和一切非振动解渐近趋向于零的若干充分条件.     

拓扑线性空间上的一致概周期性及抽象泛微分方程的Favard定理

讨论了局部凸拓扑线性空间到局部凸拓扑线性空间中映射的一致概周期性,建立了Banach空间中线性泛函微分方程的Favard分离定理。     

一类泛函极小元的H2收敛性

本文证明了一类泛函Eg(u,G)于集合h^2g(G,C)中的极小元ug当e-o时,在H^2中收敛到以g为边值的G上的双重调和映射．     

Construction of heteroduplex DNA and<Emphasis Type="Italic">in vitro</Emphasis> model for functional analysis of mismatch repair

Functional deficiency of mismatch repair (MMR) system is one of the mechanisms of tumorigenesis. With the development of the investigation and the requirement from the clinical diagnosis and treatment it is necessary to build up a method to evaluate the functional status of the whole MMR system in the concerned tumors. The original ssDNA and dsDNA from wild type (wt) bacteriophage M13mp2 and its three derivates with mutation points in the lacZα gene have been used to construct two kinds of hetero-duplex DNA molecules. One named del(2) has two bases deleted in the negative strand, the other has a G·G mismatch base pair in the negative strand too. Introducing this heteroduplex DNA into E. coli NR9162 (mutS-) without the MMR ability on the indicator plate with x-gal and IPTG, there are three kinds of plaques, mixture plaque as the characteristic phenotype of heteroduplex DNA, blue and clear plaques. If the cell extract is mismatch repair competent the percentage of the mixture plaque will decrease after incubation with these heteroduplex DNA, the repair efficiency is expressed in percentage as 100× (1 minus the ratio of percentages of mixture plaque obtained from the extract-treated sample and untreated samples), which can imply the functional status of MMR system of certain samples. After large T-antigen-dependent SV-40 DNA replication assay cell extract from TK6, a human lymphoblastoid B-cell lymphoma cell line with MMR ability, and Lovo, a human colonic carcinoma cell line with MMR deficiency have incubated with these heteroduplex DNA. The repair efficiency of TK6 to del(2) is more than 60%, to G-G is more than 50%. The Lovo efficiency to del(2) is less than 10%, to G-G is less than 20%. Therefore, in this in vitro model used for functional analysis of mismatch repair of heteroduplex DNA as the repair target, TK6 can serve as the control for MMR proficiency and Lovo as the control for MMR deficiency. Using this model the tumor tissue from a case of hereditary nonpolyposis colorectal cancer (microsatellite instability high, MSI-H) was measured and lack of MMR ability was shown. And a case of sporadic rectal cancer (SRC) (microsatellite stability, MSS) maintains MMR proficiency. The results indicate that the model is sensitive and dependable. It could be used to measure the func- tion status of MMR system in tumor cell and/or tissues. This is a reliable method to investigate the mechanic of tumori-genesis. It is meaningful in the observation of the role of MMR in the initiation and progression of concerned tumors.     

1-异丙基-1-氮杂丙炔正离子与二环烯加成反应研究

用密度泛函B3LYP方法在6—31G^ 水平上研究了1-异丙基-1-氮杂丙炔正离子[R-C=N-CH(CH3)2] 与二环[2,2,1]-2-庚烯的反应机理．优化得到各驻点的几何构型,通过振动分析和内禀反应坐标对过渡态进行了确认,解析了反应路径．并用SCRF(PCM)方法在同一水平上对标题物在CH2Cl2溶液中的反应进行了研究,计算了反应在气相和CH2Cl2溶液中的活化能垒．结果表明,该反应有TS1(△Ea=12．72kJ／mol)和TS2(△Ea=41．97kJ／mol)两条途径,产物主要通过能量较低的过渡态TS1生成．     

Relationship between toxicity and molecular volume of dioxins,organic phosphorous compounds and <Emphasis Type="Italic">n</Emphasis>-alkanols

The molecular volumes of dioxins, organic phosphorous compounds and n-alkanols were computed at the B3LYP/6-311G** level, and were then correlated with their toxicity. It was surprisingly found that there exists the so-called “critical volume of toxicity“ (CVT) in each series of compound of the present study, i.e. the toxicity increases with increase of the molecular volume when less than CVT, while decreasing with increase of the molecular volume when larger than CVT. The CVT for the three series of compounds is quite similar, ranging 0.309----0.320 nm^3. It was proposed that the ordinary linear solvation energy relationship (LSER) should be modified with respect to the new findings, and accordingly the anaesthetic potency of partial fluorinated n-alkanols was predicted.     

复线性空间上凸函数的支撑泛函

采用子空间中支撑泛函延拓的方法，构造出在复线性空间任意点上的复支撑泛函；确定在同一支撑点上复支撑泛函的数值域，从而得到复支撑泛函具有唯一性的充分必要条件。