云南独龙族7项不对称行为特征的研究

2006年9月调查了124例独龙族中学生的7项不对称行为特征（扣手、利手、叠臂、叠腿、利足、起步和优势眼）．研究结果显示：1）独龙族除起步外,其他6项不对称行为特征的出现率R型均高于L型;2）独龙族7项不对称行为特征的出现率均无性别间差异;3）独龙族与国内10个其他民族L型率的比较显示,独龙族与布依族、苗族有显著差异;4）分析了独龙族7项指标间的相关性,发现利手与起步、利手与利足、扣手与叠腿、利手与叠臂、叠腿与利足存在相关性,且R-R型组合（右型-右型）的出现率远高于L-L型组合（左型-左型）的出现率．     

佤族舌运动类型的遗传学研究

2005年10月在云南省耿马县四排山乡调查了佤族252例中学生(男135例,女117例)的舌运动类型(卷舌、叠舌、翻舌、尖舌、三叶舌).研究结果表明:1)5种舌运动类型的出现率男女性别间均无显著差异;2)佤族5项舌运动类型中卷舌基因与尖舌基因、三叶舌基因间存在互作关系;3)翻舌基因与叠舌基因间存在互作关系,翻舌基因与三叶舌基因间也存在互作关系;4)佤族与内蒙古的汉族、蒙古族和朝鲜族以及与贵州布依族等群体比较,除与布依族的三叶舌出现率存在显著差异外,其他4种舌运动类型与布依族的出现率接近,佤族与其他三个民族舌运动类型出现率除三叶舌外均存在极显著差异.     

Characterization of the Partially Folded Monomeric Intermediate of Creatine Kinase

IntroductionRecent studies of the protein folding pathway andintermediate states in vitro and in vivo haveinduced much interest in the importance ofunderstanding the propertiesof partially structuredintermediates[1 7] . Studies have suggested thatintermed…     

求解蛋白质折叠问题的改进PERM算法

在学习PERM算法的基础上，指出了影响PERM算法效率的关键因素，进而提出了一种改进的PERM算法——IPERM(Improved PERM)．计算结果表明，IPERM的计算效率优于PERM算法，并且远高于基于蒙特卡罗的MSOE算法和基于重要性抽样的SISPER算法．     

虎纹捕鸟蛛毒素-IV突变体K27A-HWTX-IV的合成和复性及其生物学活性研究

用芴甲氧羰基(Fmoc)固相多肽合成的方法在固相多肽合成仪上合成了用丙氨酸代替虎纹捕鸟蛛毒素—Ⅳ的第27位的赖氨酸的突变体K27A—HWTX—Ⅳ，通过反相HPLC对不同条件下突变体的氧化复性结果进行监测，摸索出其最佳氧化复性条件为：0．1mol／L Tris—HCl，pH8．0，GSH 1 mmol／L GSSG 0．1mmol／L的复性缓冲液，样品浓度为0．1mg/mL。复性产物经HPLC纯化并用基质辅助激光解吸飞行时间质谱法进行鉴定，相对分子质量为4050．63Da。生物学活性实验小鼠离体膈神经—膈肌标本测活实验表明：10μg/mL天然HWTX—Ⅳ阻断小鼠离体膈神经,膈肌接头传递时间为16min，10μg/mL复性后的突变体阻断小鼠离体膈神经．膈肌接头传递时间为120min，残基的替换导致突变体生物学活性比天然毒素少，证明了第27位的赖氨酸为虎纹捕鸟蛛毒素—Ⅳ活性相关的残基。     

Membrane protein folding on the example of outer membrane protein A of <Emphasis Type="Italic">Escherichia coli</Emphasis>

The biophysical principles and mechanisms by which membrane proteins insert and fold into a biomembrane have mostly been studied with bacteriorhodopsin and outer membrane protein A (OmpA). This review describes the assembly process of the monomeric outer membrane proteins of Gram-negative bacteria, for which OmpA has served as an example. OmpA is a two-domain outer membrane protein composed of a 171-residue eight-stranded -barrel transmembrane domain and a 154-residue periplasmic domain. OmpA is translocated in an unstructured form across the cytoplasmic membrane into the periplasm. In the periplasm, unfolded OmpA is kept in solution in complex with the molecular chaperone Skp. After binding of periplasmic lipopolysaccharide, OmpA insertion and folding occur spontaneously upon interaction of the complex with the phospholipid bilayer. Insertion and folding of the -barrel transmembrane domain into the lipid bilayer are highly synchronized, i.e. the formation of large amounts of -sheet secondary structure and -barrel tertiary structure take place in parallel with the same rate constants, while OmpA inserts into the hydrophobic core of the membrane. In vitro, OmpA can successfully fold into a range of model membranes of very different phospholipid compositions, i.e. into bilayers of lipids of different headgroup structures and hydrophobic chain lengths. Three membrane-bound folding intermediates of OmpA were discovered in folding studies with dioleoylphosphatidylcholine bilayers. Their formation was monitored by time-resolved distance determinations by fluorescence quenching, and they were structurally distinguished by the relative positions of the five tryptophan residues of OmpA in projection to the membrane normal. Recent studies indicate a chaperone-assisted, highly synchronized mechanism of secondary and tertiary structure formation upon membrane insertion of -barrel membrane proteins such as OmpA that involves at least three structurally distinct folding intermediates.     

1949年以前中医舌诊学术发展历程的探究

宋以前虽然有关于疾病中舌部症状的描述,但并没有形成舌诊.金代,舌苔异常被作为伤寒病中一个具有辨证意义的主症,奠定了舌诊的基础.元代出现的舌诊专著是为了证明当时的外感病新学说"火热论"而建立的诊断方法.但是,在很长一段时间内,研究舌诊的医家们却力图用已经成为外感病治疗模式的六经辨证来研究、发展舌诊.直到温病学派成熟,建立了卫气营血辨证的舌诊方法后,外感病舌诊才从六经辨证的束缚中解放出来.舌诊得以从诊断外感病扩展到诊断内伤病,理论环节是建立了舌的脏腑分部学说,技术环节是确立了舌色与脏腑、气血、津液之间的联系.这一学术转化在清末完成.清末与民国时期,舌诊被作为中西医汇通的典范,进行了较深入的研究.中西医汇通学派的研究对舌诊的推广和应用起到了十分重要的作用.     

蛋白质折叠的计算机模拟

介绍了计算机模拟蛋白质折叠问题的背景、模型和意义。在对模型问题采用Ｍｏｎｔｅ－Ｃａｒｌｏ方法和单纯遗传算法得到能量最小构象的基础上,提出了适用的混合遗传算法,并通过计算机模拟试验对三种方法作了比较。     

基于折线形滑动和SLOPE/W模拟的滑坡稳定性对比分析

为提高滑坡稳定性分析的准确性,首先采用折线形滑动面稳定性计算方法对某滑坡进行了稳定性分析,再利用SLOPE/W程序对某滑坡进行了计算机模拟,并采用Bishop法和Janbu法在考虑地下水与未考虑地下水的情况下对该滑坡进行了计算,并将计算结果与折线性滑动的稳定性进行了比较和分析,给出了该滑坡的稳定性评价.     

辽宁吕家堡子辽河群变形大理岩的超微构造研究

本文重点讨论了凤城县吕家堡子铅锌矿区变形方解石大理岩的超微构造特征。总结出不同构造部位具有不同的超微构造特征。该矿区主要经?过三期变形作用。第一期低温(<300°C±)高应变,产生走向褶皱。局部高温(400—600°C)低应变,产生翼部小褶皱(小走向褶皱);第二期高温(600℃±)低应变,产生倾向褶皱;第三期低温高应变,产生脆性与断裂构造。