Protein folding: concepts and perspectives

In this review, the main concepts of protein folding, as deduced from both theoretical and experimental in vitro studies, are presented. The thermodynamic aspects from Anfinsen's postulate, Levinthal's paradox to the concept of folding funnel as proposed by Wolynes and coworkers are described. Concerning the folding pathway(s), particular attention is brought to bear on the early steps that initiate the process in the light of the results of the fast and even ultrafast techniques presently being used. The role of structural domains as folding units is discussed. Last, from the recent studies, it can be concluded that the main rules deduced from the in vitro folding studies are valid for the folding of a nascent polypeptide chain in vivo.     

运用激励技巧培养高职学生的英语能力

高职高专学生英语学习现状不容乐观是一个普遍现象,影响高职高专学生英语学习的原因是多方面的,教学应运用激励技巧来培养高职高专学生的英语能力。主要措施有：帮助学生正确认识高职高专及就业形式；对学生进行成功激励教育；帮助学生制订个人发展计划；提高语言的诱惑力。     

Chimeras of human lysozyme and α-lactalbumin: an interesting tool for studying partially folded states during protein folding

Protein folding is an extremely active field of research where biology, chemistry, computer science and physics meet. Although the study of protein-folding intermediates in general and equilibrium intermediates in particular has grown considerably in recent years, many questions regarding the conformational state and the structural features of the various partially folded intermediate states remain unanswered. Performing kinetic measurements on proteins that have had their structures modified by site-directed mutagenesis, the so-called protein-engineering method, is an obvious way to gain fine structural information. In the present review, this method has been applied to a variety of proteins belonging to the lysozyme/α-lactalbumin family. Besides recombinants obtained by point mutations of individual critical residues, chimeric proteins in which whole structural elements (10 – 25 residues) from α-lactalbumin were inserted into a human lysozyme matrix are examined. The conformational properties of the equilibrium intermediate states are discussed together with the structural characterization of the partially unfolded states encountered in the kinetic folding pathway. Received 28 May 1998; received after revision 6 July 1998; accepted 6 July 1998     

普通高校排球项目运动技能习得的实验研究

根据“领会教学法”的基本理论，从排球选项课教学的实际情况出发，以普通高校非体育类学生为实验对象，运用以教学比赛为主兼顾基本技术练习的“完整-分解-完整”教学法，通过教学实验探讨不同教学序列对于排球项目运动技能形成的影响，旨在进一步提高排球课的授课效果，为教法改革积累实证素材．     

汉英翻译习得

汉英科技翻译是一个将汉语版的科技文体转换为英文版的科技文体的过程。科技文体的基本特点之一就是客观性。本文论述了在英译科技文体时能体现其客观性这一根本特点所常用的一些技巧和方法,尤其是被动语态的使用。     

实施核心技能教育——职业教育改革的关键

核心技能教育是在英国为首的西方国家兴起的一种职业教育模式，其适应性已被时代所证明，我国目前的职业教育规模大、种类多，其发展的方向也必须与世界接轨，必然步入职业教育培养的核心技能化。而核心技能的职业教育的改革也必须有自己的特色，在借鉴国外的经验和自身国情的基础上进行改革，来提高学生求职的核心竞争力，提高国家整体人才素质。     

攻防技击教学对武术教学效果的影响

应用统计理论，对实验组和对照组的190名学生的武术水平进行统计分析，结论是实验组学生成绩和武术水平平均优于对照组，部分指标差异有显著性或非常显著性（P〈0．R05或0．R01），武术套路运用攻防技击教学培养学生体育兴趣能提高教学效果．     

原始物理问题——培养中学生批判性思维的新途径

批判性思维在科学发展及人们的学习生活中都发挥着极其重要的作用。因此,培养学生的批判性思维就成了当今教学的一大要务。立足于协同学理论,以批判性思维培养的途径为契机,着重探索了原始物理问题在中学生批判性思维发展中所发挥的作用,并结合原始物理问题自身特点及相应的表征层次,从批判精神和批判性思维技能两个维度进行了论证。     

高校难美类体育课程教学美学渗透的路径选择

美学在难美类体育项目教学中的合理运用和有效渗透对高校体育教学改革意义重大,对教师在教学中更高层次地把握审美元素和培养学生美的思想形成与发展拓展了新的思路.通过总结分析,从教师审美修养、教学内容、教学方法和手段、多样化教学过程、教学信息反馈五个方面提出了美学渗透的途径.     

基于技能空间理论的计算机自适应测试组卷策略研究

本文从当前技能测试中存在的问题出发,通过对技能问题解决模型的分析,基于技能空间理论提出了计算机应用技能测评的计算机自适应测试组卷策略.