两种荧光染料的合成及其量子化学研究

为研究荧光染料的发光机理,以4-溴-1,8-萘二甲酸酐和N,N-二甲基乙二胺为原料,合成了两种不同取代基的1,8-萘酰亚胺染料,并以核磁共振氢谱及质谱对产物结构进行表征,测定它们的激发光谱和发射光谱.采用密度泛函理论方法,对其基态结构进行优化,同时用单激发组态相互作用方法及6-31G(d)基组优化其最低激发单重态几何结构,用合时密度泛函理论及6-31G(d)基组计算基于上述结构的激发波长和发射波长.计算结果表明,该类物质电子在基态与激发态间的跃迁,主要是电子从环外原子转移至3个六元环上,预测的光谱性质与实验结果相符合.     

Mesencephalic Progenitors Can Differentiate into TH—Positive Neurons and Improve Dysfunction in PD Rats

This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson‘s disease (PD). MPs were prepared from El1-13 rats and proliferated in the serum-free medium with basic fibroblast growth factor (bFGF) for 10 days. Cells were then collected and implanted into the striatum only--single grafts, or simultaneously into the substantia nigra (SN) and the striatum--double grafts. Twelve weeks after transplantation, 1,1‘-dioctadecyl-3,3,3‘,3‘-tetramethylindocarbocyanine perchlorate (Dil), a fluorescent dye, was microinjected into the ipsilateral striatum. The results show that double grafted MPs have more potent effects on rotational behavior than single grafted MPs. Injection of the retrograde tracer Dil into the striatum results in fluorescent-labeled cells within the intranigral grafts only in double graft subjects. These data suggest that MP transplants not only can improve rotational behavior, but also help to reestablish nigrostriatal connections, so that such transplants may become an efficient way of treating PD.     

关于m次剩余数与无k次幂因子数的混合均值

对于给定的自然数m,k≥2及任意自然数n,利用m次剩余数am(n)与无k次幂因子数ck(n)定义数论函数am(n)ck(n),研究这个新的函数的渐近性质,利用解析方法得到这个函数的几个渐近公式。     

Studies on the sructure, mechanism of green fluorescent protein and its application

The green fluorescent protein (GFP) from jellyfishAequorea victoria has unique superiority as a kind of biological label. GFP has been widely used in all fields of biology based on the recent studies on its structure, characteristic and mechanism of bioluninescence.     

荧光增白剂对苏云金杆菌制剂毒力影响的初步研究

本研究采用棉铃虫为试虫，在一定浓度的Bt溶液中加入一定浓度的荧光增白剂后，感染棉铃虫的初孵幼虫，72小时以后，对其死亡率进行统计分析，结果表明：荧光增白剂对Bt有增效作用。     

Expression of green fluorescent protein gene with baculovirus vector in insect cells

The green fluorescence of bioluminescent jellyfishAequorea victoria is due to the presence of the green fluorescent protein (GFP). To examine whether the GFP gene can be applied as a reporter gene in insect cells, a baculovirus transfer vector containing the neomycin resistance gene (neo) was established. The GFP gene was subcloned into the vector downstream of the polyhedrin gene (ocu) promoter. In the presence of G418, the recombinant virus can be purified. Expression of the GFP gene in the recombinant virus should give rise to synthesis of the GFP with a molecular weight of 30×10³ dalton, and is observable by the strong green light irradiated by ultraviolet or blue light in viable intact insect cells. The GFP produced in insect cells has typical fluorescent spectra indistinguishable from those of the purified native GFP. The GFP gene as a good reporter gene can be applied to the baculovirus-insect cell expression system. Supported by the National Natural Science Foundation of China Hu Jianhong: born in July, 1972, Master graduate student     

不同作物对苯磺隆残留敏感性室内模拟研究

采用室内模拟添加生物测定法初步研究了冀东地区花生、大豆、水稻、玉米等作物及其品种对苯磺隆的敏感性。结果表明,所有供试作物及其品种对苯磺隆均存在一定的敏感性,其中花生、大豆双子叶植物对苯磺隆最为敏感,平均ED10值分别为0.0143μg/kg,0.0181μg/kg;水稻对其耐性较强,平均ED10值为0.0684μg/kg;玉米对苯磺隆残留的耐药性最强,主根长没有受到残留的抑制作用,但侧根受到了一定的影响。同一作物的不同品种对苯磺隆的残留敏感性也有较大的差异。     

碳酸盐岩中酸不溶残余物的矿物学研究及其成因探讨

将德国南部法郎垦地区上侏罗系麻姆统碳酸盐岩中的酸不溶残余物用Atter－bery—Zylinder法分为两种组分，即大于2μm的粉砂级组分和小于2μm的粘土级组分。并各自用X-衍射分析确定了其矿物组成。研究表明，各种矿物均含有不同的成因信息。     

非矩形荧光显示单元伏安特性的理论计算问题

采用计入阴极位降的３／２次方公式计算非矩形荧光显示单元的伏安特性。分析等效二极管阳极电压３／２次方平均值Ｖｄ的相关范围及阳极有效面积Ｓｄ、栅阴距离ｄｇｃ的取值原则，指出Ｖｄ与阳极的形状无关，对非矩形阳极的电极系统也适用；Ｓｄ与ｄｇｃ应是互相垂直，如不符合，则取修正值；当算得的Ｓｄ大于阳极实有面积Ｓａ时，应实取Ｓａ值。以平板多位荧光数码管的“８”字符为例，选点测算，相当吻合，表明修正后的３／２次方公式对非矩形阳极的显示单元也适用     

云南植物药萝芙木中51种农药残留的测定

用超高效液相色谱(UPLC)、双柱毛细管气相色谱(GC)、高效液相色谱-二级质谱(HPLC-MS/MS)分析方法对云南萝芙木进行51种农药残留测定.各测定方法在一定范围内线性关系良好,准确度和精密度均符合农残分析要求,在超高效液相色谱和高效液相色谱串联二级质谱中仅检测出多菌灵,含量分别为0.012 mg·kg-1和0.018 mg·kg-1,未超出国家食品中农药最大残留限量标准,没有检出其它50种农药.