微型振荡器在电镜超薄切片样品制备技术中的应用研究

介绍了一种全新的电子显微镜下超薄切片样品制备方法 .制样过程 (固定——浸透 )全部在微型振荡器上完成 ,制样时间仅用 4～ 6h即可 ,比常规制样时间 (1 2～ 1 5 h) ,缩短近一半 .同时 ,试剂用量只需 0 .5 ml即可 ,比常规用量 (5 ml) ,减少近十倍     

感染新疆大豆的一种球形病毒的免疫电子显微诊断

研究了免疫电子显微镜技术在感染新疆大豆的一种球形病毒的诊断中的应用.预先用黄瓜花叶病毒(Cucumber mosaic virus, CMV)抗血清包被的电子显微镜载网可从大豆感病叶组织浸出液中,捕获到大量的直径为28～30 nm的球形病毒粒子,而用大豆矮缩病毒(Soybean dwarf virus, SDV)等14种直径为28～30 nm的球形病毒的抗血清以及正常兔血清包被的电镜载网却不具有在相同的大豆感病叶组织浸出液中捕获这种病毒粒子的能力,说明CMV抗血清包被的电镜载网对病毒粒子的捕获具有高度的敏感性和特异性.实验表明,经高度稀释的CMV抗血清包被的电镜载网仍能捕获到大量相关的病毒粒子;将该电镜载网贮存于4 ℃下6星期之久,仍然保持了原有的捕获病毒粒子的能力;每个样品的全部检测程序可以在约30 min内完成.敏感性高、特异性强的免疫电子显微镜技术为研究感染新疆大豆的CMV的流行规律提供了迅速、准确的手段.     

中华绒螯蟹复眼的组织学结构及扫描电镜观察

应用组织切片及扫描电镜技术研究了中华绒螯蟹复眼的形态结构，中华绒螯蟹的复眼约由21000个小眼组成，每个小眼由折射器、受纳器和反 光器组成。折射器包括角膜与晶状体，角膜覆盖在眼睛外部的表面，由复杂的几丁质组成，产生角膜的成角膜细胞位于角膜下方；晶状体由晶体细胞内部物质形成，位于角膜和感杆束之间，感杆束由小网膜细胞膜突起的微绒毛汇合形成；受纳器即小网膜；反光器由两种色素构成，即远端色素与近端色素小；小网膜细胞形成轴索，穿过基膜上的网眼，联系神经节瓣。     

L12有序结构Ni74.5Pd2Al23.5合金超点阵内禀层错的形成机制

用ＴＥＭ研究了Ｌ１２长程有序结构Ｎｉ７４．５Ｐｄ２Ａｌ２３．５合金室温变形后的位错结构．结果表明,加Ｐｄ韧化的Ｎｉ７４．５Ｐｄ２Ａｌ２３．５合金室温变形组织中存在着大量超点阵内禀层错．这些层错是从以反相畴态分解的ａ／２〈０１１〉位错通过进一步的分解和反应转变来的．层错的形成是合金变形过程中位错交互作用的二次产物,并不成为影响Ｎｉ７４．５Ｐｄ２Ａｌ２３．５合金变形和韧性的主要因素．     

Transmission electron microscopy investigation of the Ar+ ion irradiation effect in semiconductor GaAs

Room temperature irradiation effect of GaAs compound semiconductor by 100 keV Ar⁺ ions has been systematically studied by means of transmission electron microscopy. The dose dependenceoof the Ar⁺ ion irradiation and room temperature annealing effects have been investigated. The experimental results show that the structure of GaAs transforms from perfect crystalline through weakly and severely damaged crystalline to amorphous states with the increase of the irradiation dose and the damaged states are changed during room temperature annealing. Yang Xiangxiu: born in 1968, Doctor     

Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells

A new carbazole tricationic salt, 4,4′-(1E,1′E)-2,2′-(9-(2-(1-(2-hydroxyethyl)pyridinium-4-yl)ethyl)-9H-carbazole-3,6-diyl) bis(ethane- 2,1-diyl) bis(1-(2-hydroxyethyl)pyridinium) iodide (THEPC) was synthesized. Photophysical experiments have shown that THEPC has large two-photon excited fluorescence action cross-sections (33 GM in the presence of DNA), which ranks THEPC as a good biological fluorophore. The results from electronic absorption, circle dichroism and single-/two-photon fluorescence emis-sion spectra suggest that THEPC can strongly bind to DNA, with an intrinsic binding constant of 5.79 × 106 L mol–1. THEPC has better photostability under one- or two-photon excitation conditions. Finally, the staining photos from two-photon fluorescence microscopy (TPM) show that THEPC can exclusively label the nucleus with high contrast and without image distortion. These re-markable properties and optimized imaging ability make THEPC an attractive DNA probe in TPM.     

BCN薄膜的结构及其内应力研究

用射频反应溅射法制备出BCN薄膜。X射线衍射和傅立叶红外吸收光谱量发现样品的组成原子之间实现了原子级化合，样品与Si衬底之间存在较大的应力。样品剥落后，应力的消除使红外吸收峰位向低波数移动。实际还发现，衬底材料对样品中的压应力影响很大，压应力的释放与衬底密切相关：在硅衬底上呈枫叶状隆起，在石英衬底上样品呈周期性的拆线状隆起。     

Orientational domains at room temperature in La0.33Ca0.67MnO3 perovskite

Orientational domains at room temperature in orthorhombic perovskite La_0.33Ca_0.67MnO₃ were studied by group-theory and observed systematically using transmission electron microscopy. There are six orientational variants (A, A′, B, B′, C andC′) in orthorhombic perovskite La_0.33Ca_0.67MnO₃. Their orthorhombicb _o directions are parallel to thea _p,b _p andc _p directions of the cubic prototypic perovskite, respectively. In each case there are two orientational variants (e.g.,A andA′) with theira _o andc _o axes interchanged. Among the possible 15 boundaries between these 6 variants there are only two types of domain boundaries: (1) m<100>boundariesC′/C, A′/A, andB′/B. (2) m<110>boundaryiesC′/A, C′/A′, C′/B, C′/B′, C/A, C/A′, C/B, C/B′, B′/A, B′/A′, B/A, andB/A′. Foundation item: Supported by the National Natural Science Foundation of China (19974031) and US DOE(DE-AC02-98CH10886) Biography: WANG Ren-hui (1937-), male, Professor.     

Primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum

This is the first report on a primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum. These primary cells revealed a low seeding efficiency after 3 days (11.6 ± 4.6%), whereas subcultured cells had a higher seeding efficiency at the same time point (75.5 ± 34.0%) and increased in cell number (150 – 200% of seeded cells after 20 to 30 days). The cells were characterized applying histological, immunocytochemical and ultrastructural methods. The culture consisted of undifferentiated keratinocytes. Mucous cells as well as differentiated epithelial cells were absent. To date the cells were cultured for maximally 9 passages and 402 days and therefore provide the possibility for long-term studies. Received 31 March 1998; received after revision 14 July 1998; accepted 14 July 1998     

Halogen bonded two-dimensional supramolecular assemblies studied by high resolution scanning tunneling microscopy

We described the formation of self-organized two-dimensional (2D) assemblies of N-(2,3,5,6-tetrafluoro- 4-iodophenyl)hexadecylamine and 1-dodecyl-imidazole at the liquid/HOPG interface. The two-dimen- sional assemblies showed a fishbone-like pattern structure as revealed by high-resolution scanning tunneling microscopy. Although different interactions can drive the formation of 2D assemblies, as far as we know, this is the first report on halogen bond-driven 2D assemblies.