木槿幼叶培养再生植株的细胞组织学研究

以木槿(Hibiscus syriacus)幼叶为外植体,培养在附加6-BA_3+IAA(或IBA)_(0.1)+GA_(0.5)+LH_(100)的1/2MS培养基上,通过细胞组织学观察表明:接种3—10天,接触培养基的细胞体积增大,核染色加深,并移向细胞中央,有的细胞已开始分裂,接种15—20天,表皮,叶肉的栅栏细胞、海绵细胞,以及维管束附近的薄壁细胞等,都有细胞分裂,并形成愈伤组织,培养30天后,叶片结构紊乱,形成大量的分生细胞团或细胞群。同时,还有维管分子、维管组织结节,以及芽原基和叶原基的相继出现,芽原基进一步培养,形成无根苗,转移无根苗到生根培养基上,20天后,可形成完整再生植株,其结构与正常木槿植株一致。     

Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)

Cucumber (Cucumis sativus L.) is an annualclimber originated from the tropic rain forest area inthe south of Himalayas, which belongs to the Cucur bitaceae family. Cucumber is one of the importantvegetables in the world, and its planting area is sec ond only to that of tomato[1]. However genetics re search on cucumber obviously drops behind that of thelatter. The classic genetic map of cucumber, with sixlinkage groups, is composed of more than 100 genesfor color, morphology, and dise…     

Construction of a genetic map and localization of QTLs for yield traits in tomato by SSR markers

Using an F2 population derived from the hybrid of Lycopersicon esculentum Mill. ‘XF 98-7’× Lycopersicon pimpinellifolium LA2184, a SSR genetic linkage map of tomato is constructed. The map contains 112 markers and spans 808.4 cM with an average distance of 7.22 cM between loci. Two quantitative trait loci (QTLs) for first flower node on chromosomes 5 and 11, two QTLs for number of flowers per truss on chromosomes 2 and 5, and five QTLs for fruit weight on chromosomes 1, 2, 3, 9 and 12 are identified.     

慈姑雌花蜜腺的解剖学观察

应用石蜡切片和组织化学方法,对慈姑雌花蜜腺的形态结构进行了研究.结果表明,慈姑雌花蜜腺不很发达,无明显的成形蜜腺,仅存在腺细胞及分泌道,腺细胞位于最外(最下)轮雌蕊基部的花托表面,由腺细胞构成腺表皮,属于花托蜜腺.它仅有一层分泌表皮(腺表皮)而未分化出特殊的产蜜组织,又属于非结构蜜腺.腺表皮由一层近方形的大型细胞组成,细胞排列紧密,细胞质浓厚,细胞核大,内含丰富多糖类物质.根据其蜜腺的位置及结构,可以推测其蜜腺可能是由花的雄蕊退化而成的.     

樱椒树（TapisciasinensisOliv）的花果观察初报

通过对樱椒树（ＴａｐｉｓｃｉａｓｉｎｅｎｓｉｓＯｌｉｖ）开花结果过程的连续观察和部分阶段的切片观察，在本文首次报道了樱椒树的开花结果过程。樱椒树６—７月开花，从９月起幼果进入休眠期，直至来年４月，幼果重新萌动，继续发育．到９月果实成熟；其从开花到果实成熟共需１５个多月。通过切片观察初步确定合子在经过１或２次分裂后，即停止分裂，只有顶细胞越过休眠期，发育成胚，其余细胞均在停止分裂之后便逐渐解体，到休眠期时即看不到。其花托非常发达，在花期后期长大，并在周围下延，包围很短的花柄，到开花当年９月初即完全包围花柄。     

烟草复合抑芽剂及其工业生产

报导了西南民族学院化学系应用有机化学研究室研制、国家民委组织、四川省教委主持鉴定通过的烟草复合抑芽剂的性能、应用效果及其工业生产工艺,该剂是以多碳饱和醇为主的复合触杀剂型,大田试验表明以5％左右浓度施用最佳,采用打顶、抹芽、涂药同时进行,抑芽率达95％以上;生产工艺简单,适合乡镇企业生产,若按年产100吨计,年税利可达22万元以上。     

Intermediate targets and segmental pathfinding

Neurons must often extend axons over fairly long distances, making multiple changes in their trajectory of growth before arriving at their final target. It has become clear that as growth cones navigate these complex projections, they typically extend toward a number of intermediate targets before they contact their final target. Recent work from a variety of systems has identified intermediate targets that seem to play similar roles in vertebrate and invertebrate nervous system development. From these examples it appears that a general model of axon guidance can be proposed whereby neurons are guided to their targets segmentally. Within each segment, an intermediate target appears to be the primary target for growth cone recognition and thus the completion of the journey to the final target is determined by a series of successful segmental pathfinding decisions.     

不同浓度的激素组合对猕猴桃愈伤组织诱导及芽分化的影响

本实验以美味猕猴桃试管苗为材料，外植体选用离体茎段，研究了不同浓度的激素组合对猕猴桃愈伤组织诱导及芽分化的影响．试验结果表明，MS培养基中附加激素2，4-D1．0mg／L＋6-BA0．5mg／L有利于愈伤组织诱导，诱导率达86．1％；Ms培养基中附加激素组合NAA0．1mg／L＋6-BA1．0mg／L有利于芽分化，芽分化率为75．2％．     

高pH促进黄瓜离体子叶花芽分化的适宜处理时间和部位

在已知ｐＨ７．０预培养２ｄ能有效地促进花芽形成的基础上，进一步研究了其适宜的处理时间和部位。结果显示，高ｐＨ处理最有效的时间是子叶离体后的２４ｈ内，最有效的部位是子叶本身，尤其是子叶柄。同时提示ｐＨ能直接作用于子叶形态发生区域．