A preliminary study on S．asotus linnaeus and S．soldatovi meridionalis Chen by DNA fingerprinting

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A family net of giant pandas in the Tangjiahe Natural Reserve： Assessment of current individual migration

In the present study, formalin-fixed feces, oligonucleotide fingerprinting and SRY-gene based sexing were used to construct a family net for giant pandas in the Tangjiahe Natural Reserve and to assess contemporary gene flow （migration） in this population. A total of 124 fecal samples were attributed to 37 individuals （22 females and 15 males） that were then analyzed for family relationships. Based on DNA fingerprints, the deduced family net revealed the following facts： （i） First-order relatives possessed similarities from 50% to 90%, and similarities between unrelated individuals or distant relatives were as high as 77%, indicating that the Tangjiahe pandas are characterized by high genetic similarity; （ii） 15 matings were identified and 5 ones occurred between close relatives, implying that there is potential for inbreeding to impact the pandas; （iii） four mating pairs and 5 offspring presented long distance migrations, demonstrating the intra-reserve habitat is continuous; （iv） four pairs of full sibs （also female-male dyads） dispersed short distance and all of them gave birth to highly inbred offspring, reflecting long distance migration is vital for inbreeding avoidance; （v） 17 adult individuals dispersed short or moderate distance and formed three clusters on the landscape, indicating that it is necessary to find out whether there is a negative factor impacting the pandas.     

一种基于树结构的高斯分布扩频指纹嵌入技术的方法研究

数字指纹是目前保护多媒体内容不受非法拷贝分配的最新技术。一定条件下,多媒体拷贝内容能够有效跟踪非法用户并抗击共谋攻击是其应用的关键技术。在多播环境下,考虑带宽影响研究了一种基于树结构的扩频指纹嵌入系统。该系统在高斯分布情况下,对一定的共谋攻击者,系统俘获其的概率比正交扩频指纹系统提高了约40%,从而增强了追踪非法用户的能力,进一步提高了指纹加密系统的鲁棒性。     

生物识别技术与应用

生物识别技术可以用来鉴别身份或证明身份，主要包括虹膜识别、视网膜识别、面部识别、指纹识别、DNA识别、气味识别、签字识别和声音识别等，其中指纹识别是最常用的一种．生物识别技术在人类的物理生存空间和虚拟生存空间具有广泛的应用前景。在介绍生物识别原理的基础上。讨论了两种类型生物识别技术，一种基于生理特征：虹膜识别、视网膜识别、面部识别和指纹识别；另一种基于行为特征：签字识别和声音识别。     

一种基于Cox框架与LWT的彩色图像水印算法

在数字媒体交易应用中,水印算法必须符合交易协议的要求。提出一种新的彩色图像交易水印算法。算法基于Cox框架思想与提升小波变换(LWT)的特点、把卖方的版权水印与买方的数字指纹嵌入同一幅图像中,能与相应的水印交易协议相结合,实现对图像作品合法版权的鉴别、对非法盗版者的追踪。算法对一种现有小波域水印方案进行了实质性改进,显著提高了水印的不可感知性。     

Marked differentiation in a new species of dwarf stonebasher,Pollimyrus cuandoensis sp. nov. (Mormyridae: Teleostei), from a contact zone with two sibling species of the Okavango and Zambezi rivers

We studied the systematic status of a form of Pollimyrus of the lower Kwando River that is flanked by the Okavango River, inhabited by Pollimyrus castelnaui (Boulenger, 1911), and the Zambezi River, inhabited by Pollimyrus marianne Kramer et al., 2003. In morphology and electric organ discharges (EODs), the Kwando phenotype proved well differentiated from both P. castelnaui and P. marianne. Sequence analysis of the cyt b gene confirmed that the three forms or species form a monophyletic clade, with P. castelnaui sister to the other two species. Genomic fingerprinting with ISSR-PCR confirmed differentiation of the Kwando form, that we recognize as a different species, P. cuandoensis sp. nov., from its sister species, P. marianne. A considerable amount of EOD and morphological variation was revealed among samples of P. cuandoensis sp. nov. from four different locations on the lower Kwando River, possibly due to hybrid introgression. This seems an ideal system for testing theories of parapatric speciation. http://www.zoobank.org/urn:lsid:zoobank.org:pub:FB644B21-04F6-44F8-AEB6-8DC09268C17C     

安哥拉山羊及其杂种羊父权认定的DNA指纹图分析

应用ＤＮＡ指纹技术对安哥拉山羊作亲子鉴定,结果发现子代个体的ＤＮＡ指纹图中,谱带均分别来自父亲和母亲,没有新带出现。父权概率为０．９８８９,表明（ＣＡ／ＧＡＴＡ／ＴＣＣ）５探针能有效地应用于安哥拉山羊及其杂种后代的遗传分析。     

动态蜜罐技术分析与设计

针对蜜罐在配置和维护方面存在的挑战,提出了动态蜜罐技术的思想,并对它进行分析．动态蜜罐是一个即插即用的蜜罐系统,它通过监控和自学习实时的网络环境、收集网络中计算机的信息能够自动地确定应配置多少蜜罐以及怎样对它们进行配置．该蜜罐系统主要使用了被动指纹识别技术和虚拟蜜罐技术．被动指纹识别技术基于每种操作系统的IP协议栈都有其自身特点的原理,通过捕捉和分析网络中的数据包从而确定周围计算机操作系统的类型．利用虚拟蜜罐技术的思想,能够在单一的物理设备上配置多个虚拟的蜜罐系统．结合这两种技术,文章最后给出了一个动态蜜罐的设计模型,同时也分析了它的不足之处．研究结果表明,动态蜜罐能够从根本上解决蜜罐在配置和维护上存在的问题．     

淮河蚌埠段浮游生物群落RAPD指纹与水体重金属含量的相关性研究

在测定淮河蚌埠段河水重金属含量的基础上,对淮河蚌埠段浮游生物群落DNA多态性进行RAPD指纹技术分析,探讨了重金属含量与浮游生物群落RAPD指纹的相关性.结果表明,所筛选的11条随机引物共获得97条谱带,多态率为85.1%,所有引物在各位点可获得7~11条扩增谱带,平均8.9条.淮河蚌埠段河水中Mn、Fe和Pb的含量高于《中华人民共和国生活饮用水卫生标准》,Fe和Pb的含量高于世界卫生组织《饮用水标准》.Hierarchical cluster聚类分析表明,淮河蚌埠段浮游生物群落RAPD指纹与淮河蚌埠段水体重金属含量的聚类结果基本一致,浮游生物群落RAPD指纹与水体重金属浓度呈现较好相关性,为建立新的淮河水质监测与评价技术提供科学参考.