Suitability of urethane anesthesia for physiopharmacological investigations in various systems Part 1: General considerations

Summary The suitability of urethane anesthesia for physiopharmacological investigations is reviewed. Total dose administered and route of administration are recognized as factors having a great influence on both resting parameters and biological responses to drugs. A peculiar characteristic of urethane is represented by its ability to induce a surgical plane of anesthesia without affecting neurotransmission in various subcortical areas and the peripheral nervous system. This makes urethane a suitable general anesthetic for studying neural function in both central and peripheral nervous systems and accounts for the preservation of a number of reflex responses in urethane-anesthetized animals.     

The in vivo expression of the globin genes of theβ cistron in γ-,δ-, andδβ-thalassemia heterozygotes

There is considerable evidence suggesting that the switch from to and chain production after birth is due, in part, to silencing of the genes by stage-specific factors which bind to their promoters and to the competition from the adult ( and ) genes for a common enhancer element located in the locus control region. As a consequence one can expect that the increased Hb F production in adults with hereditary persistence of fetal hemoglobin or -thalassemia is directed mainly by -globin genes in cis to the deletion(s) responsible for these conditions. Here we review data on heterozygotes with -, -, or -thalassemia, who also had an^AT mutation, in cis or in trans, which was used as a marker of gene expression. The results show that a deletion affecting adult genes favors the expression of genes in cis, while the deletion of a single gene does not affect the expression of the gene in cis but leads to a faster switch postnatally.     

Detection of toxic metabolites in the hemolymph ofBeauveria bassiana infectedSpodoptera exigua larvae

Highly active metabolites have been detected in the hemolymph of the lepidopteranSpodoptera exigua infected with the mycopathogen,Beauveria bassiana. A combination of phenyl sepharose and CM ion exchange chromatography was utilized to extract the active metabolites from infected hemolymph samples. The active in vivo metabolites, having a molecular mass greater than 10 KDa, were thermolabile and were inactivated by proteinase K. These metabolites were characterized by their ability to disrupt metamorphosis, killing treated larvae at the wandering or pupal stage. Additionally, injection ofS. exigua larvae with active samples caused a reduction in the number of filopodial-producing hemocytes. The biological activities and biochemical properties suggest that novel compounds are produced duringB. bassiana mycosis.     

医院信息管理系统业务量估算与网络流量控制

本文介绍医院信息管理系统组成与设计要求;讨论了信息系统业务量估算,网络流量控制方法,保证信息管理系统数据有效性,快速存储,共享使用与安全可靠性。     

怎样加强工程造价管理

从设计阶段、招投标阶段、施工阶段及竣工阶段4个方面探讨了加强工程造价管理,实施有效的成本控制的方法与措施。     

基于VC中通信技术的研究

为了提高通信程序的开发效率 ,对SOCKET通信程序的编制技术进行了研究 ,同时介绍了TCP IP协议下如何编制通信程序的过程 ,并用VC6.0编写程序实例具体阐述 .     

科技人才收益分配模式探讨

在当前经济全球化的环镜下，企业参与市场的竞争说到底是人才的竞争．在工资收入分配上体现人才的价值属性，是促进科技人才不断激活其内在的创造潜能的关键．从“现益”和“助益”相结合的角度出发，提出了年薪制、协议工资制、专利权转让和科研项目承包、知识报酬激励、技术入股、股票期权等科技人才收益分配模式．     

多媒体计算机辅助教学在高职化学教学中的应用

在高职化学教学中,多媒体 CAI 具有无可比拟的优越性,利用多媒体 CAI 关键在于设计和制作出好的课件,并要处理好有关问题。     

购入材料短缺情况下的会计处理

企业购入材料,在办理验收入库时,如发现短缺或损耗,应查明原因,并根据不同情况及时作出不同的会计处理。     

大学基础数学教学中高层次人才的培养

论述了在大学基础数学对高层次人才培养的要求,高层次班的教学内容,教学方法,并进行了高层次班教学内容与教学质量的分析。