一种简便快速工艺制备重组人胸腺肽α1

将构建的含有胸腺肽α₁融合蛋白的编码基因插入表达质粒pET28a(+)中,并在大肠杆菌BL21中进行表达,该菌经IPTG诱导融合蛋白高表达后,离心收集菌体,超声波破碎,包含体裂解,目标蛋白质的体外变性复性后,经DEAE-Sepharose Fast Flow阴离子交换层析纯化,每升菌液中可获得260mg融合蛋白。经重组肠激酶酶切反应、Ni-Sepharose亲和层析,Sephadex G-25柱纯化后,得到了纯度达97%的重组人胸腺肽α₁,该纯化工艺简单快速,经三步层析即可得到纯度达97%的重组人胸腺肽α₁,且产量达82mg/L,为大批量用细菌表达重组人胸腺肽α₁及纯化提供了参考。     

抗冻蛋白及其对冰晶生长的影响

抗冻蛋白具有特殊的热迟滞性质,即其冰点低于熔点,且能抑制冰晶的生长．本文综述了在耐寒鱼、昆虫体内以及最近二三年在植物、真菌、细菌等生物中发现的抗冻蛋白,分析其结构及其对冰晶生长的影响,并寻求抗冻蛋白在细胞、生物组织的低温保存中可能的应用途径．     

Progress in molecular parasitology

Summary Substantial progress has been made in the last ten years in understanding the structural and functional organization of parasitic protozoa and helminths and the complex physiological relationships that exist between these organisms and their hosts. By employing the new powerful techniques of biochemistry, molecular biology and immunology the genomic organization in parasites, the molecular basis of parasite's variation in surface antigens and the biosynthesis, processing, transport and membrane anchoring of these and other surface proteins were extensively investigated. Significant advances have also been made in our knowledge of the specific and often peculiar strategies of intermediary metabolism, cell compartmentation, the role of oxygen for parasites and the mechanisms of antiparasitic drug action. Further major fields of interest are currently the complex processes which enables parasites to evade the host's immune defense system and other mechanisms which have resulted in the specific adaptations which enabled parasites to survive within their host environments. Various approaches in molecular and biochemical parasitology and in immunoparasitology have been proven to be of high potential for serodiagnosis, immunoprophylaxis and drug design.This paper is based on a review presented at a workshop on Molecular Parasitology, organized by the Swiss Society of Tropical Medecine and Parasitology at the University of Neuchâtel, March 1985.     

Malaria vaccine

Summary Among infectious diseases caused by protozoa, malaria is still the greatest killer of children. Mortality in adults living in endemic areas is significantly lower because they frequently acquire partial or complete immunity to the major pathogen,Plasmodium falciparum. This natural protection indicates that vaccination may be possible, and the first candidate antigens were cloned with the use of human immune sera as probes. Genetic and biochemical analysis of the parasite proteins revealed that they are polymorphic, and frequently gene sequences were discovered which were specific for a particular parasite isolate, which eliminated most antigens for purposes of vaccine development. The most promising candidate antigens today are the major surface proteins of sporozoites and blood stage parasites. However, the immune response against those is not sufficient for complete protection, and additional, intensive research is necessary to identify new molecules to be included in a vaccine cocktail against malaria. The current spread of the disease due to increasing drug resistance of parasites and mosquito vectors emphasizes the urgent need for a vaccine.     

运动中线粒体活性氧产生机制的研究进展

线粒体呼吸链是运动内源性活性氧(ROS)产生的主要来源,内源性ROS产生增多,可导致脂质、蛋白质及核酸等物质的氧化损伤.研究表明,ROS产生可引起质子漏,质子漏增加可降低ROS生成,提示ROS与质子漏之间存在一种反馈回路;解偶联蛋白(UCPs)参与了这种反馈调节,但其确切分子机制尚不清楚.从运动中线粒体ROS产生及其机制等方面进行了综述.     

Purification and Characterization of an Antibacterial Protein from the Cultured Mycelia of Cordyceps sinensis

0 IntroductionAlnattie mdicfrroobmiala p wriodteei nvsar iheatdy boefe linv ifnogun odr gaanndis ismos--Bacteria[1], fungi[2 ,3], plants[4]and ani mals[5].Those proteins displayed a wide spectrumof anti mi-crobial activity against different species of viruses ,bacteria andfungi .Over the past few years ,several anti microbialpeptides and proteins were foundinfungus ,such asAFP fromAspergillus giganteus[6], Anafp fromAspergillus niger[7], Zygocin fromthe yeastZy-gosaccharomyces bailii[8],an…     

用^211At和^131I标记蛋白质的研究

报道了砹—211和碘—131标记蛋白质的方法.通过过氧化氢或氯胺T直接氧化制备,使用凝胶色谱从反应产物中分离标记蛋白质,相对法比较放射性计数测定标记产率,过氧化氢适用于~(211)At标记蛋白质,氯胺T有利于~(131)I标记蛋白质.8次实验的结果表明,用过氧化氢氧化标记的~(211)At牛血清白蛋白产率96.4％,氯胺T氧化标记的~(?)I牛血清白蛋白产率66.1％.间接法标记蛋白质,放射性核素~(211)At同对氨基苯甲酸反应获得对—~(211)At—苯甲酸,经乙醚萃取和高效液体色谱分离,然后再偶联到免疫球蛋白或牛血清白蛋白上。动物实验结果表明,此种结合的标记蛋白质在体内稳定,标记率不低于初始~(211)At放射性活度的40%.     

利用Benford定理分析固有无序蛋白

目的 利用Benford定理分析固有无序蛋白(Intrinsically Disordered Proteins,IDPs).方法 利用本福特定律在生物通路动力学速率常数的成功运用所受到的启发来分析固有无序蛋白.结果与结论 首次系统地调查固有无序蛋白,并发现所有固有无序蛋白及固有无序蛋白的结构域长度首位数的分布都很好地符合本福特定律.因此,固有无序蛋白能做为一个理想的组合来研究实际生活得到的数据的首位数分布,此外,这些观察值还能用来全局地约束固有无序蛋白的行为,这也许能够为固有无序蛋白更加精确的预测提供有用的信息,也将对很多疾病的治疗提供新的思路.     

玉米巯基蛋白酶抑制剂的纯化及部分性质

从玉米种子纯化的两种巯基蛋白酶抑制剂（ＣＰＩ－Ｉ和ＣＰＩ－Ⅱ）分子量分别为９５００和１３０００,经们都只抑制巯基蛋白酶的水解活性,而对丝氨酸蛋白酶则无抑制作用,属于巯基蛋白酶水解抑制剂,它们在ｐＨ３．０～１１．０或１００℃温度范围内保有１００％的抑制木瓜蛋白的抑制活性,表明在这些外界条件下其分子非常稳定。