Effect of EGF1 peptides in directing nanoparticles to thrombi

In order to examine the effect of EGF1 peptides in directing nanoparticles to thrombi, the synthesized EGF1 peptide was conjugated to poly (ethyleneglycol)-poly (lactide) (PEG-PLA) nanoparticles (NP) to form EGF1-NP. A lipophilic fluorescent dye, coumarin-6, was incorporated into EGF1-NP so as to detect its loading and release capacity. The binding ability of EGF1-NP with TF-expressing cells was shown to be significantly higher than that of the non-conjugated NP. Following an intravenous administration, fluorescence was distributed along the vessel wall of the thrombosis regions in the model rats injected with coumarin-6-loaded EGF1-NP. The in vitro and in vivo results suggest that EGF1-NP is a promising drug delivery system for targeting cerebral thrombi.     

基于“虚拟物流”理念的文献传递协作平台建设

分析了＂虚拟物流＂理念运用到文献传递服务中的可行性,阐述了＂虚拟物流＂在文献传递协作平台中的应用模型,提出了基于＂虚拟物流＂理念的文献传递协作平台运行模块的设置。     

论儿童诗歌对儿童的影响

儿童诗歌是指为儿童创作的、符合儿童的心理特点和审美习惯的诗歌。儿童诗歌以其积极向上的思想内容、鲜明和谐的节奏韵律和语言形式,以及自身所具有的美感,在引领儿童体验健康高尚的感情、培养儿童的审美能力、提高儿童对文学的兴趣和鉴赏水平方面,能够起到其他文学作品所起不到的作用。     

使用病毒载体的罕见病基因疗法临床进展

 很大一部分的罕见病由遗传因素决定,难以用普通的小分子或大分子药物治愈,而基因治疗有望从根本上修正人体功能的缺失或异常,给罕见病患者带来改善生活质量的希望。目前许多基因疗法的临床试验正在开展,病毒载体是常用的基因递送方法,本文讨论了用于临床基因递送的多种病毒载体,包括腺相关病毒、逆转录病毒和慢病毒,重点列举了这些病毒在罕见病临床试验中的研究、应用和进展,评价了这些病毒的优缺点,并简述了基因疗法的研究方向及应用前景。     

Micro/nano needles for advanced drug delivery

Along with the boost of drugs’ exploitation, drug delivery has been a common concern among researchers and patients. As an emerging strategy, the micro- and nano-needle (MNN) is a single or an array of micro/nanoneedles, and promises to be a robust technology for efficient, noninvasive and convenient drug delivery. Compared with the conventional injection, MNN can pierce the bio-membranes to transfer cargos without any damage of tissue or nerves. And regarding the topical drug release, MNN is more efficient than oral administration and topical cream. More specifically, microneedle, a powerful technique, has been widely applied in different diseases such as diabetes, anti-obesity and contraception through skin and ocular surface. Correspondingly, many novel microneedles and optimization methods have been developed recently to improve the drug delivery efficacy. Nanoneedle, much smaller than microneedle, can minimize the skin damages caused by the repeated use of microneedles. Nanoneedles with nanoscaled size are usually used to penetrate the cell membrane. And recently many stimulus-aided methods like electrical and magnetic force have been invented to deepen penetration and improve transfer effects. The aim of this review is to introduce the recent advances of MNN-based drug delivery, including their types, application locations and diseases, optimization of delivery strategies and future development prospects.     

同时送取货的随机旅行时间车辆路径问题方法

建立了同时送取货的随机旅行时间车辆路径问题(STT-VRPSPD)的机会约束规划模型,构建了分散搜索算法求解策略.分散搜索算法中,针对STT-VRPSPD问题的复杂特性,构造了解的改进策略、组合策略,并采用改进的节约算法构造分散搜索算法初始解,从而使文中设计的分散搜索算法更加适应STT-VRPSPD问题特有的负载波动性.仿真实验中,首先对分散搜索算法的参数设置进行分析,确定了最优参数组合;然后基于经典的Dethloff算例数据,构造了STT-VRPSPD的测试算例,并对分散搜索算法和遗传算法进行了对比分析,结果表明,分散搜索算法对于STT-VRPSPD的求解质量优于遗传算法.     

需求稀少情况下随机动态装卸混合问题研究

针对随机动态装卸混合问题中存在的排队现象,运用排队论推导出需求稀少情况下随机动态装卸混合问题期望系统时间的下界;提出了一种实时优化策略——多车场随机队列中位策略;推导出需求稀少情况下,多车场随机队列中位策略和实际应用中广泛采用的随机队列中位策略的期望系统时间,并分析了期望系统时间的渐近性.模拟计算结果表明,需求稀少情况下,多车场随机队列中位策略明显优于随机队列中位策略;当服务强度趋于零时,多车场随机队列中位策略近似为最优策略.     

烟草物流输送线上分箱装置的设计与应用

成品物流自动输送系统上的烟箱在进入提升机等节点设备工位易出现卡烟箱现象。设计了一种输送线烟箱分隔装置,对机械装置中的驱动方式、横档材料以及横档结构进行了优化设计,采用检测定位、PLC技术实现电气控制设计,最终使进入提升机的件烟箱保持一定合理间距。经实际应用,改进前平均每月卡烟箱5~6次,改进后完全杜绝了卡烟箱现象,提高了设备运行效率。     

论非婚生子女强制认领制度——兼评婚姻法解释三第二条第二款

非婚生子女强制认领制度应当以未成年子女最佳利益与身份安定为原则.强制认领主要包括直接强制与间接强制.婚姻法解释三第二条第二款填补了我国亲子关系强制认领制度的空白,具有积极意义,但是条文规定略显粗糙,于具体适用仍存在诸多困难,如主体范围不明,适用推定缺乏明确标准以及未对子女进行成年与未成年的区分等.基于“未成年子女最佳利益原则”与“身份安定原则”,适用解释三第二条第二款应把握以下三方面：限制提起认领之诉的主体;应当有基础的证据;对子女进行成年与未成年的区分.     

孟鲁司特钠咀嚼片对儿童过敏性哮喘前瞻性研究

目的探讨孟鲁司特钠对儿童过敏性哮喘的临床疗效.方法筛选过敏性哮喘儿童92例,在征得患儿监护人同意的情况下采用随机双盲法分为治疗组(47例)和对照组(45例),两组患儿的基本治疗方案均相同.治疗组患儿在基础治疗上加服孟鲁司特钠咀嚼片,对照组患儿服用相同剂量的安慰咀嚼片,在治疗3个月后比较两组患者的疗效,随访6个月观察是否出现复发,同时统计患儿EOS,FEV1和PEF的相关变化情况.结果治疗组患儿临床有效率为97.87%,与对照组比较有统计学意义(P<0.05);治疗组患儿临床体征改善情况也较对照组有所提高,差异具有统计学意义(P<0.05).两组患儿肺部功能指标均有所改善,但治疗组较对照组改善更为显著,差异具有统计学意义(P<0.05);两组患儿在治疗过程中均未发生损伤性药物不良反应,治疗组患儿复发率较对照组低.结论孟鲁司特钠可作为儿童过敏性哮喘的辅助治疗药物,且服用安全,疗效显著.