中国人HLA-G1的cDNA克隆、序列分析、表达和蛋白质空间结构预测

从中国人外周血单个核细胞、胎盘组织和肝癌组织等样品中克隆了包含完整HLA-G1读框的cDNA;与国外同行获得的该基因及其蛋白质序列比较分析表明,该基因虽然有着细微的种族特异性,但高度保守;并获得了它的截断型重组蛋白,根据蛋白一级结构和同源比较方法,模建了它及其与特异性受体KIR2DL4形成复合体的空间结构模拟,预测了它们之间相互作用的特征.     

单模动力系数第三大n—周期的存在性和唯一性

讨论关于单模动力系统，迭代是动力系统中最简单的模型，这些一维模型呈现很好的数学结构，某函数是区间[0,1]上具有连续自映射的单模凸函数F（x)，且该系统还依赖于某一特征实参数λ∈[0,1]。利用逆序列函数hn-1(λ)的性质，证明了对于单模凸函数F(x)第三大n-周期的MSS序列P＝RL^n-4R^2，存在某一自然数n0,当自然数n不小于该自然数时，则在某一区间（λRL^n-4R,λRL^n-4)中，一定存在与该MSS序列相对应的特征实参数λRN^N-4R^2，并且该值具有唯一性。     

非线性映象的Mann迭代的收敛性与不动点

在可度量化拓扑线性空间中，讨论一些非线性映象的不动点与Mann迭代序列的收敛性问题，在一定条件下，得到了一些新的结果，推广和发展了Khan,Ghosh及Rhoades等人的工作。     

Banach空间中一类φ-强增生算子方程的解及其迭代逼近问题

 在Banach空间中研究了一类 φ -强增生算子方程的解的存在性及其逼近问题,所得结果推广和改进了已有文献的相关结果.     

非天然D-型氨基酸生物转化中酶的纯化及其基因序列

D-海因酶 (D- Hydantoinase,HDTase )和 N -氨甲酰基 - D-氨基酸酰胺水解酶 (D- Carbam oylase,DCase )是医药和食品工业上用于生物转化 D-型氨基酸的酶。为了用人工酶替代现用的天然酶转化工艺 ,我们用热变性、硫酸铵分级及 Q - Sepharose、Phenyl- Sepharose、Superose12等多步柱层析 ,从现用菌株 No.2 2 6 2中纯化了 HDTase和 DCase,二酶均达到 SDS- PAGE纯 ,经 N -端序列分析 ,HDTase和 DCase的 N -末端氨基酸序列分别为 :MDKL IKNGTI和 MTRQKIL AF。首先 ,根据酶的 N -末端氨基酸顺序推论并合成了正向多核苷酸简并引物 ,然后按蛋白质数据库资料 ,在比较不同菌株氨基酸残基同源性基础上 ,化学合成了数对反向多核苷酸引物 ,以菌株 No. 2 2 6 2基因组 DNA为模板 ,经 PCR扩增获得了 HDTase的部分基因序列 (～ 70 0 bp )和 DCase的全部基因序列 (912 bp ) ,为使人工酶用于非天然 D-型氨基酸的生物转化奠定了基础。     

A genetic algorithm on multiple sequences alignment problems in biology

The study and comparison of sequences of characters from a finite alphabet is relevant to various areas of science, notably molecular biology. The measurement of sequence similarity involves the consideration of the possible sequence alignments in order to find an optimal one for which the “distance” between sequences is minimum. In biology informatics area, it is a more important and difficult problem due to the long length (100 at least) of sequence, this cause the compute complexity and large memory require. By associating a path in a lattice to each alignment, a geometric insight can be brought into the problem of finding an optimal alignment, this give an obvious encoding of each path. This problem can be solved by applying genetic algorithm, which is more efficient than dynamic programming and hidden Markov model using commomly now. Foundation item: Supported by Zi-qiang Foundation of Wuhan University and Open Foundation of the State Key-Laboratory of Software Engineering, Wuhan University Biography: Shi Feng(1966-), male, Associate professor, research direction: bioinformatics.     

Identification of protein superfamily from structurebased sequence motif

The structure-based sequence motif of the distant proteins in evolution, protein tyrosine phosphatases (PTP) Ⅰ and Ⅱ superfamilies, as an example, has been defined by the structural comparison, structure-based sequence alignment and analyses on substitution patterns of residues in common sequence conserved regions. And the phosphatases Ⅰ and Ⅱ can be correctly identified together by the structure-based PTP sequence motif from SWISS-PROT and TrEBML databases. The results show that the correct rates of identification are over 98%. This is the first time to identify PTP Ⅰ and Ⅱ together by this motif.     

Identification of a novel DNA molecule associated with Tobacco leaf curl virus

A circular DNA molecule, designated as DNAβ, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAβ sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAβ consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβ has little sequence homology to DNA-A of TLCV., except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%–65%) with the reported DNAβ molecules associated with Ageratum yellow vein virus and Cotton leaf curl virus. The immunotrapping PCR and whitefly transmission tests showed that DNAβ molecule could be encapsidated in virus particle and transmitted by Bemisia tabaci. This is the first report of DNAβ associated with WTGs in China.     

洪浩尔舒特凹陷非构造油气藏的形成及勘探前景——以洪东洼槽为例

以洪东洼槽为例 ,根据其钻井剖面、地震测线及测试资料 ,结合油气勘探现状 ,运用沉积学、层序地层学等多学科理论 ,以沉积、构造演化入手的油气地质动态综合分析法为思路 ,对洪东洼槽的基本成油条件进行深入分析 ,总结其油气勘探成功、失利的原因和油气分布规律 ,寻找有利勘探方向 .结果表明 :早白垩世阿尔善期湖盆的碎屑物质主要来自陡岸 ,在纵向上形成多套自生自储式含油组合 ;在平面上形成多期继承、叠加连片的非构造圈闭 .最后指出阿上段、阿中段是油气钻探的主要目的层段 ,努格达构造带下倾部位是非构造油气藏形成的有利部位和有利勘探方向     

准噶尔盆地昌吉凹陷西斜坡侏罗系层序地层特征

在对地震、岩心、钻井、测井及分析化验资料综合分析的基础上 ,研究了准噶尔盆地昌吉凹陷西斜坡侏罗系的层序地层特征。结果表明 ,工区内侏罗系本身构成了一个由低水位、湖侵和高水位体系域组成的二级层序 (超层序 ) ,超层序的顶、底界面分别是侏罗系与三叠系、侏罗系与白垩系的区域性不整合面。根据层序内局部不整合面的分布范围、地层叠置样式 ,识别出 4个三级层序。由于侏罗系沉积时期受振荡性构造运动的影响 ,三级层序的发展不完整。除了层序 1发育低水位和湖侵体系域外 ,其他 3个层序均由湖侵和高水位体系域组成。超层序的低水位体系域 (相当于八道湾组下段 )发育湿地扇扇前河流平原沉积体系。湿地扇辫状沟道中的粗粒碎屑岩是该区的有利储层 ,与上覆煤层或炭质泥岩配置可形成优质储盖组合。湖侵体系域发育三角洲或滨浅湖沉积 ,高水位体系域发育辫状河沉积。