运输企业发展现代物流的探索与分析

介绍了临汾市运输企业向现代物流转型所采取的措施及取得的成果,阐述了临汾市物流中心的规划与建设的详细内容,并提出了推动现代物流发展的几点思考。     

智能抽油机节能控制器的研制

设计了一种新型智能抽油机节能控制器，通过检测电机的功率因数间接判断井下供液能力的大小。本控制器可以延长低产油井的开采寿命，减少各机械部件的磨损，有效降低用电成本。     

混装生产物流动态调整数学模型

研究了热－热混装（ＣＣ－ＤＨＣＲ与ＣＣ－ＨＣＲ混合）和冷一热混装（ＣＣ－ＤＨＣＲ与ＣＣ－ＣＣＲ混合）的生产物流动态调整问题，以库存缓冲特征增量模型为基础，建立了不同生产情况下的混装生产物流动态调整模型。     

带二维装箱约束的需求可拆分车辆路径问题模型及算法研究

车辆配送计划时通常会考虑货物易损、易碎的可能性,以及如何充分利用有限的运输资源对货物进行配送以满足顾客的需求。在车辆有限、货物易损坏、顾客需求可进行拆分等环境下,实现车辆配送总路径最短,即考虑二维装箱约束的客户需求可拆分的车辆路径问题。对问题进行详细定义,建立了2L-SDVRP模型。将遗传算法与BLF算法的结合求解模型;并用数值案例验证算法有效性。     

罗非鱼冷链物流中的异常温度检测方法研究

受冷链物流系统和设备的影响,冷链物流中的温度很可能出现异常,导致罗非鱼变质。当前异常温度检测方法检测精度和性能低,不适于实际应用。为此,提出一种新的罗非鱼冷链物流中的异常温度检测方法,给出罗非鱼冷链物流中异常温度数据的判据,介绍了模糊ARHMM的结构。通过中间计算结果从三个不同方面确定罗非鱼冷链物流中的温度数据是否异常,把各部分正确的运算数据集看作一个隶属度函数,以此描述待检测温度数据在该集合的隶属度;通过结合得到的三个检测集确定最终隶属度,从而实现罗非鱼冷链物流中的异常温度检测。实验结果表明,采用所提方法对罗非鱼冷链物流中的异常温度进行检测,检测精度和整体性能均较高。     

涉及第三方物流服务质量的供应链协调

在考虑信息完全共享且需求随机条件下,引入第三方物流服务提供商(third party logistics service provider,TPLSP)在物流运作中出现的货损货差因素,建立了由供应商、TPLSP和分销商组成的三级供应链独立决策模型和集中式决策模型.设计了收益共享契约,通过算例的仿真计算与分析,验证了实际情况中货损货差成本往往由TPLSP独自承担所造成的对TPLSP激励不足的问题.进一步证明了选择恰当的收益分配系数能够使供应链达到帕累托最优,同时也能激励TPLSP为减少货损货差付出努力;并给出了最优订货批量、TPLSP和供应商的协调定价以及收益分配系数的取值范围.     

顾客导向视觉下物流服务质量的集对分析

结合物流服务的特点,从顾客感知角度建立物流服务质量评价指标体系,利用熵值法确定各指标的权重,基于集对分析方法构建物流服务质量评价模型,并以湖州市某第三方物流企业为例验证该模型的实用性和可行性,实例表明集对分析方法的评价结果较为合理,且具有概念清晰、计算方法简洁、可操作性强的优点,对其他研究的评价具有一定的借鉴意义。     

基于灰色关联的浙江省现代物流业发展因素分析

物流业作为国民经济中重要的基础性产业之一,对推动区域经济高效协调发展起着非常重要的作用．运用灰色关联分析方法,对影响浙江省现代物流业发展的因素进行定量研究,分析了促进浙江物流业发展的关键驱动因素和未来进一步发展的突破口,最后提出了有针对性的政策建议．     

Preparation and cellular uptake of PLGA particles loaded with lamivudine

Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles loaded with lamivudine and coated with bovine serum albumin (BSA) were prepared via a double emulsion method. The influences of experiments parameters such as volume of inner aqueous phase, concentration of organic phase and ultrasonication time on the particle size and drug entrapment efficiency were investigated, obtaining PLGA particles with a diameter of ~260 nm and drug entrapment efficiency of ~35%. The particles were observed by scanning electron microscopy and transmittance electron microscopy, showing a core-shell structure. BCA assay found that 58 mg BSA was present on/in 1 g LPB particles. The loaded lamivudine showed a burst release at beginning and sustained release until 24 h in physiological conditions. Low pH could accelerate the release of lamivudine from PLGA particles, making the PLGA particles potential intelligent intracellular drug carriers. The PLGA particles were readily internalized into the human liver cells within a short time and increased gradually with the prolongation of incubation time regardless of the loading of lamivudine. The particles either resided within lysosomes or transferred to cytoplasm, but could not enter into the cell nucleus. The cell viability was not significantly influenced in the presence of the particles regardless of lamivudine encapsulation, suggesting that this kind of particles may be a good candidate for the intracellular anti-hepatitis B drug delivery.     

Enhancement of the efficiency of PEI/liposome transfection by magnetofectins formed via electrostatic self-assembly

One of the major challenges for successful gene therapy is improving the transfection efficiency of non-viral vectors. Magnetic nanoparticles (MNPs) have been developed as enhancers of non-viral vehicles. We prepared MNPs and modified them with polyethyleneimine (PEI), citric acid (CA) or carboxylmethyl-dextran (CMD). Both positively charged MNPs (MNPs@PEI) and negatively charged MNPs (MNPs@CA, MNPs@CMD) could spontaneously form transfection complexes (magnetofectins) with plasmid DNA and PEI/liposome via electrostatic self-assembly. Our results showed as-prepared magnetofectins apparently enhanced PEI/liposome transfection efficiency and/or gene expression level into COS-7 cells with reduced transfection time from 4 h to 15 min under a magnetic field in vitro. Meanwhile, the effect of magnetofection was cell line-dependant. These results suggest that charged MNPs could improve transfection efficiency for non-viral vectors by simply mixing with them and by exerting a magnetic force. Thus such MNPs provide a convenient platform for further applications of gene delivery.