Thrombospondin co-localises with TGFβ and IGF-I in the extracellular matrix of human osteoblast-like cells and is modulated by 17β estradiol

Thrombospondin (TSP) is a multifunctional glycoprotein which is synthesised by several cell types including osteoblasts, and incorporated into the extracellular matrix (ECM) of these cells. The function and regulation of TSP in bone is not clear. In this study, using a long term culture model of human osteoblast-like cells, we examined the distribution of TSP in the ECM and its modulation by added estradiol. In this model the osteoblast-like cells form a regular multilayer which continues to increase in depth up to 50 days post confluence. In the ECM of these cultures and in 19-week fetal bone, the bone markers osteocalcin and alkaline phosphatase were diffusely distributed in the matrix. In contrast, labelling for TSP was concentrated, confined to the banded collagen and its immediately adjacent ECM. This pattern of labelling resembled that of the growth factors transforming growth factor-I (TGF), and insulin-like growth factor-I (IGF-I), with which TSP label co-localised. Labelling intensities were comparable between fetal bone and the in vitro material for TSP, TGF and IGF-I. TSP label was present by 10 days post confluence, reached a maximum by 20 days, and declined slowly thereafter, a time course which was similar to that of IGF-I. Incubation of osteoblast-like cell cultures with 17 estradiol resulted in an increase in multilayer depth and a maximal 3-fold increase in TSP labeling at 30 days as well as approximately 2-fold increases for TGF and IGF-I. The dose-response relationship for these responses to estradiol treatment was biphasic with maximal increases at 10^–10 M–10^–11 M of added estradiol. Treatment with 17 estradiol produced labelling intensities that were not significantly different from controls. Studies with other cell types have suggested that TSP may be involved in modulation of growth factor activity. The similarities between TSP, TGF and IGF-I, in terms of their distribution and regulation by 17 estradiol treatment, may indicate a role for TSP in modulating bone cell proliferation and function through interaction with local growth factors.     

桂东南马尾松工程幼林生长量及其生物量节律研究

１９９１年～１９９６年,对桂东南马尾松工程幼林的生长量及生物量进行连续的定位定期观测。结果表明：马尾松工程林６年生幼林的林木蓄积量和生物量分别为３６．１６ｍ３／ｈｍ２和３６．８６ｔ／ｈｍ２,分别比常规造林１１年生和不施肥同龄林增加３倍～６倍;揭示了马尾松幼林生长量规律及其影响的主要气象因子,马尾松工程幼林各器官生物量随年龄的变化规律,并分别建立与地径逐月生长量的回归预测模型。     

唐家河自然保护区扭角羚种群动态及稳定性研究

１９８６年唐家河自然保护区内扭角羚的实际数量为４８０－５２０头,作者通过Ｌｅｓｌｉｅ矩阵的方法,预测了该保护区的内扭角羚种群１９８６－２００４年每隔３年各年份的理论数量分别为７１５,９６４,１３５８,１８４１,２５６４,３５８４头。讨论了该保护区扭角羚最适数量的应控制在１９９２年的９６４头,最大维持量不得超过１９９５年１３５８头。     

大熊猫的放归与保护

根据大熊猫在野外的分布格局,对栖息地的选择及种群生存力的分析,应将大熊猫就地保护,使固有的栖息地的种群得以恢复,并提高其生存值和适应度。另一方面,圈养的大熊猫存活率和净生率都大于野外种群,经过一段时间的增殖,保持其野外生态习性,通过驯化,先在大面积范围内散放,然后放归自然,增加野外种群数量和遗传多样性是可行的。     

Effects of extracellular calmodulin on pollen germination and tube growth

The effects of calmoddin (CaM) antagonist W7-agarose, anti-CaM serum and exogenous purified CaM on pollen germination and tube growth ofForsythia suspensu were studied. The pollen germination and tube growth were inhibited or completely stopped by CaM antagonist W7-agarose. The addition of exogenous purified CaM stimulated pollen germination and tube growth, whereas the same amount of bovine serum albumin (BSA) had no effect. The inhibitory effects caused by W7-agarose and anti-CaM serum could be reversed completely by the addition of exogenous purified CaM. The tube growth of germinated pollen was also inhibited or completely stopped by W7-agarose. The results suggest that endogenous extracellular CaM initiates pollen germination and tube growth, whereas exogenous CaM enhances the above processes.     

高温氧化膜Cr2O3和NiO中应力松弛过程

用Ｘ射线原位应力测定了高温氧化膜Ｃｒ２Ｏ３和ＮｉＯ中的应力．结果表明,Ｃｒ２Ｏ３／Ｃｒ和ＮｉＯ／Ｎｉ体系氧化膜的生长应力分别为－１．９４９ＧＰａ和６５２ＭＰａ．在冷却过程中,Ｃｒ２Ｏ３／Ｃｒ体系发生很大的应力松弛过程,其主要表现形式为氧化膜翘曲和开裂;而在ＮｉＯ／Ｎｉ体系中,由于ＮｉＯ薄膜具有双层结构承受不同应力状态而获得松弛．     

Endothelial Cell Culture on Different Phospholipid Surfaces *

ＩｎｔｒｏｄｕｃｔｉｏｎＢｉｏｍａｔｅｒｉａｌｓａｒｅａｌｗａｙｓｕｓｅｄｉｎａｎｅｎｖｉｒｏｎｍｅｎｔａｄｊａｃｅｎｔｔｏｌｉｖｉｎｇｔｉｓｓｕｅｓａｎｄｂｌｏｏｄ．Ｃｅｌｕｌａｒｉｎｔｅｒａｃｔｉｏｎｓｗｉｔｈｉｍｐｌａｎｔｓａｒｅｏｆｐｒｉｍａ...     

生长曲线模型中协差阵的一致最小方差非负二次无偏估计

在准椭球等高分布下给出了生长曲线模型中ｔｒ（ＣＶ）的一致最小方差非负二次无偏估计存在及任一个非负二次估计成为一致最小方差非负二次无偏估计的充要条件。     

4种植物生长物质及温度胁迫对绿豆黄化幼苗肌醇磷脂代谢的影响

利用放射性标记和薄层层析（TIC）分离发现2,4－D,GA3和6－BA可不同程度地促进肌醇－1,4,5－三磷酸（IP3）水平的增加．在最适浓度下的作用效果是：GA3＞2,4－D＞6－BA．但脱落酸（ABA）没有引起IP3含量的变化．低温（0℃）和高温（35℃）胁迫亦引起IP3含量的增加．2,4－D,GA3,6－BA和温度胁迫的共同处理对IP3含量的增加有协同作用．表明2,4－D,GA3,6－BA和温度胁迫促进绿豆黄化幼苗肌醇磷脂代谢,ABA也许不参与绿豆黄化幼苗肌醇磷脂代谢．     

随机载荷下大型构件疲劳裂纹扩展寿命的预测

本文从影响疲劳裂纹扩展随机性的外因（载荷条件）和内因（材质的分布规律）着手,探讨它们的交互作用对随机载荷作用下构件疲劳裂纹扩展的影响,采用数值模拟的方法,对桥梁、船舶、飞机、特殊容器等大型构件所承受的实际载荷谱以及材料对裂纹扩展的阻力系数等进行模拟,并进而对疲劳裂纹的随机扩展过程进行模拟,提出随机载荷作用下大型构件疲劳裂纹扩展寿命的预测及其可靠性分析方法．