东营凹陷沙三段-沙二下亚段T-R层序特征及成因

将东营凹陷古近系沙三段沙二下亚段划分为 3个三级层序 ,即沙三下层序 (SSⅠ )、沙三中层序 (SSⅡ )和沙三上沙二下层序 (SSⅢ )。SSⅠ和SSⅡ层序由湖进体系域和湖退体系域组成T R旋回 ,其特征是代表层序边界的不整合分布局限 ,湖进体系域厚度小 ,主要为细粒沉积物 ,退积式叠加 ;湖退体系域较厚 ,由进积式叠加的砂岩、砾岩和泥岩组成。T R层序的形成主要是由于层序形成早期的构造沉降和气候起主导作用 ,其基底快速沉降 ,湖水供应充分 ,湖平面快速上升。在层序形成晚期 ,沉积物供给和气候起主导作用 ,沉积物供给多 ,快速向前推进 ,湖平面下降。构造沉降和沉积物供给因素对层序控制作用的转换促使其形成层序和体系域的边界。T R层序与三分层序之间的转换是由于层序形成的不同阶段的主控因素不同而引起的     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

沥青路面罩面的设计方法

针对国内外罩面或改建设计方法的不足 ,借助于同济大学对新建和罩面改建后的沥青路面使用性能的研究成果 ,将寿命周期费用分析引入罩面设计 ,提出了沥青路面罩面设计方法———基于使用性能和技术经济分析的最佳罩面时机和最佳罩面厚度选择方法 ,并通过实例说明了该方法的应用     

喷涂掘进煤巷抑制瓦斯涌出的研究

通过喷涂煤壁可抑制瓦斯涌出原理，建立了瓦斯在煤体与喷涂层中渗流传质的物理数学模型，得出煤壁喷涂前后瓦斯渗流压力的分布及瓦斯涌出量随煤层透气系数的变化规律，提出了煤层透气系数的测定方法及喷层厚度的计算方法，揭示出喷层厚度与喷层的透气性及喷层与煤壁的粘结应力的相互关系。     

步进电机细分驱动中绕组电流的修正

在步进电机细分驱动系统中，由于步进电机绕组电流与磁场幅值之间的非线性关系，以及步进电机转子的磁滞效应等因素，细分后得不到均匀的步距角，因此必须对细分电流的大小进行修正，讨论了修正的方法，并给出了修正后的实验数据。     

JT6100SD新型双层客车操纵稳定性

针对大型客车的结构特点,分析了影响大型客车操纵稳定性的因素,并对相关参数进行了计算。计算结果均满足相关规范要求,提出了进一步改善其操作稳定性的建议措施。     

虚拟企业生命周期分析与系统管理

虚拟企业是一种新的合作模式,它可以适应不断变化的市场环境,因此自从它被提出后就受到企业界和学术界的广泛关注,并取得了一系列的研究成果。虚拟企业是由两个或两个以上的独立企业组成的一种临时性网络组织,当期望目标达到后此组织即解散。成功地实施虚拟企业可为企业带来可观的收益,但实施虚拟企业战略也有许多风险。作者在分析虚拟企业生命周期的基础上,提出了虚拟企业生命周期系统管理策略。     

影响变频空调性能的因素

变频空调的原理主要是利用电能与热能两能转换而达到调温目的,变频空调具有节能和良好的软启动性能,同时使用变频空调还受其他因素影响,故选购变频空调时要视具体情况而定。     

RTK快速求解整周模糊度算法及应用

分析了GPS-RTK测量速度的关键即解算整周模糊度，通过对RTK整周模糊度算法原理的分析，提出了快速RTK求解整周模糊度算法．该算法首先确定未知点的初始坐标，进而得到初始模糊度；然后以初始值为中心建立一个搜索空间，应用最小二乘原理搜索出准确的模糊度值．