B12基因在小鼠部分肝切除后的表达差异分析

B12是一种受TNF-α诱导的蛋白．利用肝脏部分切除小鼠模型以及核酸酶保护反应研究了B12基因在肝脏部分切除后不同时间的表达差异，发现在受损后28h，B12的表达开始增强，到36h表达一直呈上升趋势，推测该基因可能与肝脏修复过程中DNA的复制有关，为B12基因的进一步的功能研究提供了理论基础．     

地骨皮降血糖效果研究及成分分析

地骨皮为茄科落叶灌木枸杞的干燥根皮，多年来在中国民间被用于治疗肺炎、咳血、肢端坏疽及糖尿病等。本实验采用水提和醇提法分别对地骨皮有效成分进行提取，获得冻干粉。利用四氧嘧啶腹腔注射构建糖尿病模型，将实验鼠分为4组：正常对照组、糖尿病对照组、地骨皮低剂量组和高剂量组。将低、高剂量组小鼠每天分别灌胃100mg／kg，200mg／kg地骨皮水溶液，用药28天，每周监测血糖和体重变化，结果地骨皮处理组血糖水平与糖尿病阳性对照组比明显降低（p〈0．01），降低率达36．72％（低剂量组），41．89％（高剂量组）；体重与阳性对照组相比明显增加（P〈0．05）。第29天，取血测各组三脂酰甘油（TG）、胆固醇（TC）水平，结果与糖尿病对照组比二者均明显下降。再通过薄层色谱技术对地骨皮的各种成分进行鉴定，比较出两种提取方法上成分的差异，实验表明地骨皮具降糖作用，其主要成分是有机酸和生物碱类。     

生川乌水煎液抗肿瘤作用的实验研究

以小鼠肉瘤S180细胞造模，设模型对照、川乌水煎液高、中、低剂量组共四组，灌胃给药10 d后观察生川乌水煎液的抑瘤作用,并应用四甲基偶氮盐(MTT)法观察生川乌水煎液在体外对肿瘤细胞LoVo、MGC-803的生长抑制作用.实验证明，生川乌水煎液可显著抑制小鼠S180实体瘤的生长，对肿瘤细胞LoVo、MGC-803的生长有明显的抑制作用.     

Synthesis dynamic and developmental profile of prothoracicotropic hormone between diapause- and nondiapause-destined individuals in Helicoverpa arrnigera

Biosynthesis and secretion of prothoracicotropic hormone （PTTH） of diapause- and nondiapausedestined individuals in Helicoverpa armigera were studied using whole-mount immunocytochemistry and enzyme-linked immunosorbent assay （ELISA）. The immunocytochemistry revealed that PTTH is expressed in two pairs of lateral neurosecretory cells of the brain. The presence of immunoreactivity has not significant difference between the brains of the diapause- and nondiapause-destined 6th instar larvae. However, the obvious differences of expressional pattern from day 4 pupae were observed between the two types. PTTH titers in hemolymph from the 6th instar larvae to pharate adults were measured by the ELISA. Although there were similar titer changes between the two types of individuals at the larval stage, a significant difference from developmental expression was detected at the pupal stage, suggesting that the expression and secretion of PTTH does play a crucial role in regulation of pupal diapause of H. armigera.     

用荧光双链引物特异扩增并定量核酸

描述了一种利用特殊的双链引物－－突触引物，用于核酸扩增的特异定量检测，在传统的引物的5'端标记荧光物质，而在引物的互补序列的3'端标记荧光淬灭剂以封闭其延伸。二者杂交即成双链突触引物。在制备PCR反应混合物阶段以及加热的初期，突触引物保持稳定的双链结构而不能引导扩增，在退火阶段，突触引物部分解链导致引物延伸，荧光物质与淬灭剂分开而产生荧光。利用人β珠蛋白基因对此方法进行了验证。这种可自行退火的荧光引物不仅简单地实现了实时扩增，同时比常规的热启动PCR更有效地提高了扩增的特异性。     

Neuronal growth inhibitory factor inhibits pheochromo-cytoma PC12 in vitro

Neuronal growth inhibitory factor (GIF),named Metaliothioneins-Ⅲ (MT-Ⅲ), is the first protein validated to be capable of inhibiting the growth of neurons in nervous system. We have detected the effects of recombinant GIF on the growth of neuroblastoma SY5Y and pheochromocytoma PC12 by the MTT (Thiazolyl blue) reduction assay. Recombinant GIF inhibited PC12 in vitro; the inhibitory rate was about 25% when GIF was at 100 mg/L; and the inhibitory rate was about 50% when GIF was at 300 mg/L. It is shown that PC12 could serve as a proper model for detecting neuronal growth inhibitory activity of GIF. Recombinant GIF did not inhibit neuroblastoma SY5Y in vitro, a common model of neuroma; it is also shown that GIF could not inhibit neuromata extensively. The reason for GIF inhibiting PC12 may be that PC12 have some properties of cholinergic neuron. It must play an important role in discovering the mechanism of GIF's neuronal growth inhibitory activity.``     

一种放线酮化合物含量的几种测定方法比较

分别采用生物测定法、分光光度法和高效液相色谱法(HPLC)检测云南放线酮(4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxy-ehthy]-piperidine-2,6-dione)的含量,结果表明在这3种方法中,生物测定法灵敏度较后二法低,但能反应真实的生物学效应,并具有不需特殊设备,操作简便的特点,适用于处理样品量较多的定性和定量测定;分光光度法检测速度快、检测灵敏度较高,适用于中等样品量的定量测定;而HPLC法检测准确、灵敏度最高,则适用于样品量较少的精确定量测定.     

米诺的尔对兔晶体上皮细胞增殖的影响

研究米诺的尔(Minoxidil)对体外培养的兔晶体上皮细胞增生的影响。将不同浓度的米诺的尔加入体外培养的兔晶体上皮细胞,分别作用24、48及72 h,采用MTT法观察米诺的尔对兔晶体上皮细胞增生的影响。将3.0 mmol的米诺的尔加入体外培养的兔晶体上皮细胞作用72 h,而后吸出药液,加入正常DMEM培养液继续培养96 h,观察米诺的尔在撤药96 h后对兔晶体上皮细胞增殖的抑制作用;并应用光学显微镜观察兔晶体上皮细胞在含或不含有3.0 mmol米诺地尔的培养液培养72h后的形态学变化。米诺的尔对兔晶体上皮细胞增生有抑制作用,呈浓度依赖性和时间依赖性。3.0 mmol米诺的尔对兔晶体上皮细胞增殖的抑制作用在撤除药液后并未降低,抑制作用持续增加。说明米诺的尔能有效抑制体外培养的兔晶体上皮细胞增生。     

棘球蚴抗原纯化对免疫球蛋白IgG免疫应答的影响

以纯化的棘球蚴抗原和未经纯化的粗抗原用于棘球蚴感染者血清中抗棘球蚴抗原的特异性免疫球蛋白IgG水平的检测试验,结果表明,当血清稀释度为1∶200时,使用纯化抗原可以获得较高的特异性 (98.9%),并可保持在使用粗抗原及血清稀释度为1∶400时获得的较高的敏感性（93.7%）,提示在棘球蚴感染者血清中抗棘球蚴抗原特异性免疫球蛋白IgG水平的检测中,使用纯化棘球蚴抗原和粗抗原均可获得较高的敏感性(93.7%)和较高的特异性 (98.4%,98.9%),使用纯化抗原时的特异性略高,但在统计学上并无显著差异.     

应用单细胞凝胶电泳技术测定农药对蚯蚓的DNA损伤

探讨采用当前国际上较先进的ＤＮＡ损伤检测技术－单细胞凝胶电泳技术,来检测农药对蚯蚓ＤＮＡ的损伤,由于蚯蚓是陆生生物与土壤生物之间传递污染物的桥梁,能很好地指示土壤环境的变化,是评价农药对生态环境安全性的一项重要指标。尝试建立蚯蚓的单细胞凝胶电泳来评价农药对生态环境的影响,结果表明两种新型农药对蚯蚓的ＤＮＡ均有损伤作用。认为蚯蚓的单细胞凝胶电泳技术能敏感地农药对细胞ＤＮＡ的损伤,并可作为农药对土壤污