日本血吸虫体壁抗原基因的筛选与克隆

为筛选并克隆新的日本血吸虫(Schistosoma japonicum,Sj)候选抗原基因,以日本血吸虫成虫总DNA为模板,通过生物信息学以及分子生物学手段从日本血吸虫全基因组中筛选目的基因.根据所筛选到的目的基因的核酸序列设计合成引物,用PCR法扩增目的基因,将其克隆入pBS-T载体后转入到大肠杆菌DH5a菌株,筛选阳性克隆.通过对阳性菌落质粒进行PcR予以证实.最终成功筛选并克隆到日本血吸虫体壁候选抗原基因.     

能量存储系统双向变换器控制建模及快速实现

城市轨道交通节能与电压补偿对能量存储系统控制提出较高要求.为快速设计其控制,提高开发效率,利用Simulink/Stateflow建立了基于超级电容的城轨能量存储系统模型来快速开发其控制算法,给出了Stateflow特点分析与优化建议.在简要描述算法模型功能与通过仿真验证算法后,自动生成了适合数字信号处理器(DSP)的嵌入式代码.总结了基于DSP的快速开发流程,给出了加快控制开发迭代措施,对加快双向直流(DC/DC)变换器过渡过程也做了简述.最后,试验验证了生成代码的有效性.此控制快速开发方法同样适用于城轨其他电力电子控制系统,对降低开发难度,加快进度有较好的参考价值.     

Directional news impact curve

The directional news impact curve (DNIC) is a relationship between returns and the probability of next period's return exceeding a certain threshold—zero in particular. Using long series of S&P500 index returns and a number of parametric models suggested in the literature, as well and flexible semiparametric models, we investigate the shape of the DNIC and forecasting abilities of these models. The semiparametric approach reveals that the DNIC has complicated shapes characterized by nonsymmetry with respect to past returns and their signs, heterogeneity across the thresholds, and changes over time. Simple parametric models often miss some important features of the DNIC, but some nevertheless exhibit superior out‐of‐sample performance.     

指纹图像增强算法研究

指纹图像增强在指纹图像预处理过程中有着非常重要的作用,直接影响指纹识别的识别率和识别速度。一般图像增强算法无法满足指纹识别系统的要求,为了提高指纹增强算法的综合性能,现提出一种优化的Gabor滤波方法以增强指纹图像并有效消除噪声,并对Gabor滤波器的参数进行量化。实验表明,该算法能够使图像的质量明显得到增强,方便了后续指纹特征的提取,以提高指纹识别的算法效率和准确率。     

SARS病毒的S1蛋白基因的克隆、表达及其ELISA检测方法的建立

为了建立方便、敏感和特异的SARS病毒血清学诊断方法,利用PCR扩增,克隆了SARS冠状病毒S蛋白基因中从第68～918位碱基的基因序列(S1蛋白).并通过pGEX-4T1表达系统在大肠杆菌BL21中高效表达了SARS病毒S1蛋白,用超声波破碎菌体及用不同浓度的尿素洗涤包涵体,纯化了S1蛋白,纯度可达75%以上.以在E.coli高效表达的S1蛋白为抗原,以辣根过氧化物酶(HRP)标记的羊抗人IgG为二抗,建立了检测SARS抗体的间接ELISA方法.经检测,筛选出最佳反应条件为5μg/孔.用纯化的E.coli表达的抗原包被酶标板,用5 g/L的小牛血清进行封闭,以正常E.coli裂解上清液稀释待检血清.实验表明,应用表达的蛋白作为诊断SARS抗原具有特异性高、抗原易纯化和成本低等特点.     

黑曲霉F246的phyA基因克隆及其新型表达载体构建

利用PCR法直接从自行筛选、鉴定的黑曲霉F246中扩增出phyA基因,再将PCR产物重组于乳酸菌表达载体pMG36e,构建phyA基因的新型表达载体pMG36e-phyA.以黑曲霉F246基因组为模板,对phyA基因的成熟肽(1 347 bp)进行PCR扩增.再将PCR产物克隆入pMD18T质粒,经DNA测序和氨基酸分析、鉴定正确后,亚克隆入乳酸菌表达质粒pMG36e,构建工程菌Lactobacillus MG1363-pMG36e-phyA.重组乳酸菌表达载体pMG36e-phyA的构建,可望获得大量、高活性植酸酶,为研制多功能微生态制剂奠定基础.     

Effect of strain rate on the compressive deformation behaviors of lotus-type porous copper

Lotus-type porous copper was fabricated by unidirectional solidification, and compressive experiments were subsequently conducted in the strain rate range of 10-3–2400 s-1 with the compressive direction parallel to the pores. A GLEEBLE-1500 thermal-mechanical simulation system and a split Hopkinson pressure bar (SHPB) were used to investigate the effect of strain rate on the compressive deformation behaviors of lotus-type porous copper. The influence mechanism of strain rate was also analyzed by the strain-controlling method and by high-speed photography. The results indicated that the stress-strain curves of lotus-typed porous copper consist of a linear elastic stage, a plateau stage, and a densification stage at various strain rates. At low strain rate (< 1.0 s-1), the strain rate had little influence on the stress-strain curves; but when the strain rate exceeded 1.0 s-1, it was observed to strongly affect the plateau stage, showing obvious strain-rate-hardening characteristics. Strain rate also influenced the densification initial strain. The densification initial strain at high strain rate was less than that at low strain rate. No visible inhomogeneous deformation caused by shockwaves was observed in lotus-type porous copper during high-strain-rate deformation. However, at high strain rate, the bending deformation characteristics of the pore walls obviously differed from those at low strain rate, which was the main mechanism by which the plateau stress exhibited strain-rate sensitivity when the strain rate exceeded a certain value and exhibited less densification initial strain at high strain rate.     

Elaboration of AlSi10Mg casting alloys using directional solidification processing

The effects of pulling velocity on the solidification behavior and microstructural parameters of AlSi10Mg alloys prepared in a Bridgman-type directional solidification furnace were investigated. The microstructure, particularly the secondary dendritic arm spacing (SDAS), and the Brinell hardness (BH) of the solidified AlSi10Mg alloys were characterized for samples with cylindrical shapes and different conicities (θ=0°, 5°, and 10°). Microstructural studies revealed an increased density of α-Al phase dendrites and a decreased interdendritic distance with increasing pulling velocity. The dendrites were found to be preferentially oriented along the pulling direction for low pulling velocities. For larger pulling velocities, the dendrites grew first in the cooling direction but then broke as others nucleated and coarsened. The HB values of the solidified samples increased as the pulling velocity increased. In regard to sample conicity, smaller dendrites were observed for an apex angle of θ=5°, resulting in the largest HB value. This result was interpreted in terms of the favorable orientation of the dendrite along the pulling direction.