一类高阶复微分方程组亚纯允许解的探讨

利用亚纯函数的Nevanlinna值分布理论和方法,研究了一定条件下复域内高阶微分方程组亚纯允许解的估值问题,对一类特殊的高阶复微分方程组允许解的估计做了改进.     

2,3-Dihydroxybiphenyl dioxygenase gene was first discovered in Arthrobacter sp. strain PJ3

Bacterium strain PJ3,isolated from wastewater and identified as Arthrobacter sp. bacterium based on its 16S rDNA gene,could use carbazole as the sole carbon,nitrogen and energy source. The genomic library of strain PJ3 was constructed and a positive clone JM109(pUCW402) was screened out for the expression of dioxygenase by the ability to form yellow ring-fission product. A 2,3-dihydroxybiphenyl dioxygenase(23DHBD) gene of 933 bp was found in the 3360 bp exogenous fragment of pUCW402 by GenSCAN software and BLAST analysis. The phylogenetic analysis showed that 23DHBD from strain PJ3 formed a deep branch separate from a cluster containing most known 23DHBD in GenBank. Southern hybridization confirmed for the first time that the 23DHBD gene was from the genomic DNA of Arthrobacter sp. PJ3. In order to test the gene function,recombinant bacterium BL21(pETW-8) was constructed to express 23DHBD. The expression level in BL21(pETW-8) was highest compared with the recombinant bacteria JM109(pUCW402) and strain PJ3. We observed that 23DHBD was not absolute specific. The enzyme activity was higher with 2,3-dihydroxybiphenyl as a substrate than with catechol. The substrate specificity assay suggested that 23DHBD was essential for cleavage of bi-cyclic aromatic compounds during the course of aromatic compound biodegradation in Arthrobacter sp. strain PJ3.     

The c-myc coding DNA sequences of cyprinids （Teleostei： Cypriniformes）： Implications for phylogeny

The family Cyprinidae is one of the largest fish families in the world, which is widely distributed in East Asian, with obvious difference in characteristic size among species. The phylogeneUc analysis of cyprinid taxa based on the functionally important genes can help to understand the speciation and functional divergence of the Cyprinidae. The c-myc gene is an important gene regulating individual growth. In the present study, the sequence variations of the cyprinid c-myc gene and their phylogenetic significance were analyzed. The 41 complete sequences of the c-myc gene were obtained from cyprinids and outgroups through PCR amplification and clone. The coding DNA sequences of the c-myc gene were used to infer molecular phylogenetic relationships within the Cyprinidae. Myxocyprinus asiaticus （Catostomidae）, Misgurnus anguillicaudatus （CobiUdae） and Hemimyzon sinensis （Homalopteridae） were assigned to the outgroup taxa. Phylogenetic analyses using maximum parsimony （MP）, maximum likelihood （ML）, and Bayesian retrieved similar topology. Within the Cyprinidae, Leuciscini and Barbini formed the monophyletic lineage respectively with high nodal supports. Leuciscini comprises Xenocyprinae, CuItrinae, East Asian species of Leuciscinae and Danioninae, Gobioninae and Acheilognathinae, and Barbini contains Schizothoracinae, Barbinae, Cyprininae and Labeoninae. Danio rerio, D. myersi and Rasbora trilineata were supposed to separate from Leuciscinae and Barbini and to form another lineage. The positions of some Danioninae species were still unresolved. Analyses of both amino acid variation with parsimony information and two high variation regions indicated that there is no correlation between variations of single amino acid or high variation regions and characteristic size of cyprinids. In addition, the species with smaller size were usually found to be basal within clades in the tree, which might be the results of the adaptation to the primitive ecology and survival pressure.     

模糊以致精确

本文论述翻译理解及表达中对精确信息与模糊信息的处理,阐明语言的模糊性与翻译转换的精确性应该是相辅相存、相互渗透的,在一定条件下可以实现转化。因而翻译时对语言精确性和模糊性不可偏废、不可夸大,才能制成好译文。     

江苏汉族人生长激素受体基因多态性研究

为了解生长激素受体(GHR)基因多态性在江苏汉族人群中的分布,通过多重PCR及PCR-RFLP的方法对269例江苏地区正常汉族人进行GHR基因3个位点的多态性分析,并与文献中报道的其它种族和地区的人群进行比较分析.结果显示江苏地区汉族正常人群中GHR基因型以fl/fl、I/L、P/P发生频率最高,这种分布在男女之间没有差异,与其它地区汉族人比较没有明显差异,与日本人差异也不明显,而与法国、俄罗斯人差异明显.研究表明,GHR基因3个位点多态性在不同种族之间分布存在着明显的差异,这种差异有可能是导致不同种族间人体测量学差异及对一些疾病的易感性及对药物的反应性不同的因素之一.     

一类二阶非线性矩阵微分方程的振动性定理 (英)

建立了二阶非线性矩阵微分系统 $ (a(t)\X’(t))’+b(t)\X’(t)+\Q(t)f(\X(t))= 0,t\geqslant t_ 0 >0$ 的振动性标准, 这里 $\Q(t),$ $f’(\X(t))$ 是 $n \times n$ 矩阵， $f’(\X(t))$ 正定, $a(t)$ 和 $b(t)$ 实值函数. 引进了一个特殊函数 $\phi(t,s,r)=(t-s)^ \alpha (s-r)^ \beta , \alpha,\ \beta > \frac 1 2 $\ 是常数，$ \ r \geqslant t_0,$ 得到了形式为 $\lim \sup\lambda_ 1 [.] > $ const 的振动性标准, 改进了一些已知的结果.     

基于广告和质量的商誉模型研究

针对供应链的制造商和零售商的广告合作问题，将质量因素引入商誉模型，并运用微分对策中的马尔可夫纳什均衡分别分析了基于不考虑质量因素模型与考虑质量因素模型的制造商与零售商的利润，并将结果进行了比较，结果显示，重视质量发展可提高成员的利润．然后，运用最优控制分析了合作广告，并将合作广告的通道总利润与非合作条件下的总利润进行比较，结果显示，合作广告不仅可提高供应链的利润而且可促进质量的发展．     

重组人脂联素基因构建、表达、纯化及活性鉴定

根据脂联素cDNA序列设计并合成9条寡聚核苷酸片段,经重叠PCR技术获得重组人脂联素基因.构建pET-22b( )/ADPN表达载体,转化大肠杆菌BL21(DE3),IPTG诱导表达.目的蛋白以包涵体的形式表达,表达量占菌体总蛋白的30%以上.表达产物通过Ni-NTA柱纯化,SDS-PAGE分析显示,其分子量约为30 ku,与预期结果一致.内皮细胞生长抑制实验证实,重组人脂联素生理浓度下具有抑制人内皮细胞生长的活性并呈时间依赖性.以上工作为进一步研究脂联素的生物学功能奠定了基础.