紫露草雄蕊毛技术对水质的突变性研究初探

用紫露草雄蕊毛突变技术对盘龙江松花坝至滇池河段的水质进行监测．监测结果显示：整条江水质呈现出轻重不一的污染段面．北仓、滇池汇水口样点水质具有较高的雄蕊毛突变率，突变率分别为０．４９２‰和０．５５６‰．城区河段紫露草雄蕊毛突变率较低，最低点为双凤桥样点，突变率为０．２２６‰．但从整体来看，由松花坝雄蕊毛突变率０．０９１‰至滇池汇水口处增至０．５５６‰，水体的潜在性致突变物污染程度逐渐加重．这表明盘龙江水体可能存在致突变物污染的潜在危险性     

Thymidine kinase gene mutation leads to reduced virulence of pseudorabies virus

To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virulence, thymidine kinase (TK)-deficient mutant of pseudorabies virus strain Hubei (PRV HB) was isolated by selection for resistance to 5-bromodeoxyuridine. The tk genes of PRV HB and its TK^― mutant were cloned and sequenced. 1587 base pairs of the tk gene and flanking regions of wild-type (wt) virus were sequenced, which included an open reading frame (ORF) of 1098 bp encoding a protein of 366 amino acids. The ORF contained two 137-bp repeated sequences, which were connected by an adenosine. 1458 bp of the tk and flanking regions of TK^― mutant were sequenced. Analysis of the tk gene sequence of TK^― mutant indicated that one of 137 bp repeated sequence and the connecting adenosine in the tk gene of the wt virus was deleted and a repeated sequence of 8 nucleotides (GCGCGCC) was inserted. All other nucleotides of TK^―mutant were identical to that of wt virus. Deletion and insertion of the nucleotide sequence resulted in a frameshift and a premature chain termination, and the resultant TK protein was not active. Analysis of the amino acid sequence revealed that TK protein of PRV HB contained the conserved consensus sequence of herpesviral TKs and an additional conserved-DHR-motif. The results of this work also indicated that TK^― mutant was genetically stable. Compared to PRV HB, virulence of TK^― mutant was greatly decreased. Mice vaccinated with TK^― mutant were completely protected against a lethal challenge with virulent PRV (HB).     

关于Sato定理的一个注记

去掉了Sato定理中的一个条件,并将该定理推广到一般情况.     

黑腹果蝇NDA修复功能缺陷型细胞系的建立及其培养特性

通过低温(18℃)培养方法,成功地建立了黑腹果蝇DNA修复功能缺陷型细胞系。该细胞系的特点是只能进行开放培养而不能进行封闭培养,为研究真核生物细胞DNA修复机理提供了一个有用的实验材料。     

拟南芥抗旱突变体的筛选和鉴定

文章采用不同浓度的甘露醇模拟干旱胁迫,以发芽率为筛选指标在拟南芥突变体库中筛选抗旱突变体,筛选出2株候选突变体,最终得到1株稳定突变体vem1,通过表型和生理生化鉴定,确定为抗旱突变体。该研究为抗旱基因克隆及功能分析奠定基础,对于揭示植物抗旱的分子机理具有重要的理论意义。     

最小范数在秩亏自由网平差中的应用

为了比较经典自由网平差与秩亏自由网平差解法的特点,本文在分析秩亏自由网平差方法的基础上应用最小二乘法和最小范数法对同一水准网进行了解算,结果表明最小二乘解不唯一,权逆阵也不唯一,但改正数唯一;最小范数解与最小二乘解的改正数相同且结果唯一。     

大麦类病斑突变体bspl1的遗传及生理生化分析

对甲基磺酸乙酯（EMS）诱变大麦品种ZJU3获得的一个类病斑突变体（bspl1）进行了遗传和生理分析. 遗传分析结果显示,该表型由隐性单基因控制;苗期叶片生理生化分析表明,bspl1中SOD、POD以及CAT的活性较野生型ZJU3均明显升高. 通过比较bspl1和野生型ZJU3在生理生化水平上的差异,推测类病斑的产生与叶片中活性氧的堆积相关.     

大麦类病斑突变体 bspl 1的遗传及生理生化分析

对甲基磺酸乙酯（EMS）诱变大麦品种ZJU3获得的一个类病斑突变体（bspl1）进行了遗传和生理分析。遗传分析结果显示,该表型由隐性单基因控制;苗期叶片生理生化分析表明,bspl1中SOD、POD以及CAT的活性较野生型ZJU3均明显升高。通过比较bspl1和野生型ZJU3在生理生化水平上的差异,推测类病斑的产生与叶片中活性氧的堆积相关。     

山西产煤县农民贫困现状与脱贫对策——以武乡县、宁武县为例

山西省是我国的能源大省,有国家级贫困县35个、省级贫困县17个,约占全省总县数的50%,其中80%的县煤炭资源丰富.在这些煤炭资源丰富的县中,农民的收入与县域经济增长的关联性并不大,出现了"县富民贫"的现象.本文以宁武县和武乡县两个国家级贫困县为研究对象,运用多维贫困理论分析了两县十年来贫困的原因,提出了两县的脱贫对策,以期为山西省贫困县脱贫致富提供决策参考.