水稻矮化突变体差异表达cDNA片段的克隆与分析

利用抑制性消减杂交（Suppressive subraction hybridization,SSH）分离本实验室获得的一株水稻矮化突变体dwarf69与其野生型对照品系特光矮-2（Rryza sativa,TGA-2 indica）间表达有差异的cDNA片段,分别以dwarf69作为驱赶子,TGA-2为检测子,以及以dwarf69作为检测子,TGA-2为驱赶子建立了两个差异表达cNDA文库,分别代表在TGA-2和dwarf69中特异表达的cDNA,经反式Northern检测这两个文库共得到10个阳性克隆。     

冬小麦辐射诱变为春性农大94118的选育研究

本文通过对冬小麦品种PH82-2-2辐射诱变处理，在无零下温度条件下，获得了春性突变后代，选育出农大94118品系，试验结果还指出，冬小麦辐射诱变后获得的春性突变体，性状变异幅度大，突变谱宽，供选择的余地大，是春小麦育种的有效途径之一。     

Mapping and characterization of a tiller-spreading mutant<Emphasis Type="Italic">lazy-2</Emphasis> in rice

Tiller angle of rice is an important agronomic trait that contributes to breed new varieties with ideal architecture. In this study, we report mapping and characterization of a rice mutant defective in tiller angle. At the seedling stage, the newly developed tillers of the mutant plants grow with a large angle that leads to a “lazy“ phenotype at the mature stage. Genetic analysis indicates that this tillerspreading phenotype is controlled by one recessive gene that is allelic to a reported mutant la. Therefore, the mutant was named la-2 and la renamed la-1. To map and clone LA, we constructed a large mapping population. Genetic linkage analysis showed that the LA gene is located between 2 SSR markers RM202 and RM229. By using the 6 newly-developed molecular markers, the LA gene was placed within a 0.4 cM interval on chromosome 11, allowing us to clone LA and study the mechanism that controls rice tiller angle at the molecular level.     

重组人睫状神经营养因子突变体的制备及PEG修饰

通过大肠杆菌重组表达人睫状神经营养因子突变体(CNTFm),并进行PEG修饰,旨在降低免疫原性.该突变体将天然CNTF的C端15个氨基酸删除,大肠杆菌表达的CNTFm以包含体形式存在,经复性、纯化获得纯度达到95%的目的蛋白.体内生物学活性测定结果显示,给药10 d,小鼠最大体重减少率达31%,产生的最高中和抗体滴度达到1:6 400; 经PEG修饰,CNTF突变体的生物学活性降低了34%,但最高中和抗体滴度降低到1:800.该PEG修饰后的CNTFm制备工艺有望为CNTF的临床应用开辟道路.     

高活力天冬氨酸酶的分离提纯及性质

采用基因工程技术构建具有较高活性的突变菌株 Ｔ３３， 以它为产酶菌株分离提取天冬氨酸酶， 并研究它的酶学特性． 结果表明， 分子量为 １９０ ０００． 最适反应 ｐ Ｈ＝ ８０， 最适反应温度为 ３７ ℃． 在ｐ Ｈ＝ ６０ 时， 该酶呈负协同效应； 在ｐ Ｈ＝ ８０ 时， 该酶呈正协同效应． 该酶α螺旋度为３６３％     

Deficient functions of the random function

Two kinds of random functions are studied. It is proved that they have no deficient function almost surely.     

Construction and genetic analysis of mutator insertion mutant population in maize

A total of 26718 M1 plants were ob- tained by crossing the active mutator transposon donor parents (Q105, WW51, 115F, V26-2 and 919J) with the recipient parents (Hz85,W328 with Bz gene and S-Mo17Rf3Rf3). The phenotypes of M1 plants were observed in the field. M1 plants were self-pollinated to develop the mutator insertion-mutagenized M2 seeds. The transposition frequency of the mutator in the genome was calculated based on the spotted aleurone phenotype of the M2 seeds. The results showed that: (1) the mutation frequency of M1 phe- notypes in the field was 0.07 in the population of W328×Mu; (2) the mutation frequency of spotted aleurone seeds on the M2 ears was 0.122 in the population of W328×Mu; (3) five S-cytoplasm male-sterile plants were found among 22500 M1 plants of S-Mo17Rf3Rf3×Mu, with the transposition frequency about 2.2×10?4 per locus. 99 flanking se- quences of mutator transposition were amplified by the modified MuTAIL-PCR, and 59 non-redundant sequences with length around 400 bp were obtained. After bioinformatic analysis, 27 sequences of them could be annotated, using non-redundant nucleotide database of maize, rice, and Arabidopsis. 36 se- quences of them were located on the genetic map of maize by comparative genomics, and several flank- ing sequences of mutator insertion were mapped on the single marker locus. Hotspot sequences of mu- tator transposition were revealed by comparing the homologies between the 9-bp target site duplication of the mutator insertion. The putative functions of 8 flanking sequences of mutator transposition had identity with the functions of their correspondingmarker. The constructed mutator insertion mutant population in maize will facilitate the new gene discovery and functional genomics study in maize.     

Dirichlet级数的唯一性定理和随机Dirichlet级数的亏函数

证明了D irichlet级数的唯一性定理,并研究了全平面上的有限级随机D irichlet级数,证明了有限级的随机D irichlet级数几乎必然(a.s.)没有亏函数.     

一类高阶线性微分方程解在角域上的增长性

主要研究了高阶微分方程 f（k）+ Ak -1 f（k -1）+…+ A1 f ＇+ A0 f =0的解在角域上的增长性,其中 A0,Aj （1≤j≤k -1）为亚纯函数,且假设 A0以有限复数 a 为亏值,ρ（Aj ）=0（1≤j≤k -1）,通过给定适当的条件,证明了齐次线性微分方程的任一非零解在某些角域上的增长级为无穷。