银合欢壳多糖酶在转基因Khao Da wk Mali 105籼稻中的表达(英文)

通过含有pCAMBIA1300载体(抗潮霉素基因(hpt)作为选择标记基因)的根癌农杆菌菌株EHA105的介导,将326个银合欢I型碱性壳多糖酶基因(KB3,GenBank登录号为:AAM49597)的氨基酸导入到籼稻Khao Dawk Mali 105(KDML 105)植株中,获得了KDML 105籼稻的愈伤组织。pCAMBIA1300载体包含有CaMV35S启动子、银合欢壳多糖酶基因和NOS终止子。在NB培养基的水稻秧苗中愈伤组织的转化率为94%.在培养温度为28℃,以含有4 mg/l BAP和7 g/l植物凝胶再生培养基的条件下,愈伤组织的再生频率高达80%.为了建立一个KDML 105农杆菌转化方法,考察了各方面的因素。共培养期间,在培养基中添加50μM的乙酰丁香酮对提高瞬时转化率非常重要。4周龄的盾片衍生的愈伤组织是最佳的起始材料。添加40 mg/l潮霉素和400 mg/l头孢霉素的NB培养基是水稻愈伤组织选择性转化的最佳培养基,其转化率达到78%.采用PCR、Northern Blot杂交凝胶、Southern Blot杂交凝胶和GUS分析技术研究了愈伤组织和转基因水稻中的壳多糖酶和gus基因的表达。壳多糖酶和gus基因在水稻的多个部位被检测到,包括愈伤组织、叶、根、茎和秧苗,但是它们在水稻根部的含量较少。抗真菌的水稻壳多糖酶表现出抗串珠镰刀菌的性质。     

First-principle study of interaction of H2 and H2O molecules with (ZnO)n(n=3?6) ring clusters

A density functional calculation was performed to investigate the impact of hydrogen and water molecules on zinc oxide clusters (ZnO)n=3–6 ··· X, where X=H2 and H2O. The calculated binding energies were corrected for the basis set superposition error (BSSE). The structural parameters and chemical hardness were calculated for the complexes of zinc oxide clusters and guest molecules. The strength values of the interaction between the clusters and the guest molecules were analyzed based on the topological properties of atoms in molecules (AIM) theory of Bade r. The stereo electronic interact ions inside the ZnO clusters were analyzed in detail using the natural bond orbital (NBO) analysis.     

基于三维自治系统复杂网络的混沌保密通信系统

将一个三维自治系统作为网络节点, 研究以该混沌系统为节点的全局耦合网络渐近同步问题. 用混沌掩盖的方法将其应用于保密通信中, 通过加入Gauss白噪声对系统进行干扰, 并用小波分析理论对信号进行去噪处理. 数值仿真结果表明, 经小波阈值去噪后, 可无失真地恢复有用信号.     

复亚正定矩阵的行列式不等式

研究复矩阵的正定性,无论对于理论或应用都有重要价值,是矩阵论中重要的热门课题.建立了复亚正定矩阵的一系列行列式不等式,获得了一些新的结果,改进并推广了Ky-Fan、Ostrowski-Taussky、Openheim和Hadamard等著名不等式.     

Scientific heritage in Brazil

This work presents an overview of Brazil’s scientific heritage, especially the collections and sets of artefacts related to the exact sciences and engineering. The information provided is the outcome of a survey being undertaken on a national level under the coordination of Museu de Astronomia e Ciências Afins (Museum of Astronomy and Related Sciences, or MAST), which is leading teams from five Brazilian universities. Sets of objects have been identified at museums, universities, military establishments, and some secondary schools. The best preserved collections are at a few museums, but the universities hold most of the artefacts. The overwhelming majority of the objects were made in the twentieth century, primarily the second half. After the general results of the survey are presented, details about a few sets of objects and collections are given, including information about their current state, the provenance of the objects and the history of the institutions. The objective of this initiative is to raise the awareness of the Brazilian state so that a policy is created for preserving this heritage and financing mechanisms to assure it can be researched, conserved, and ultimately fulfil its mission in society.     

反de Sitter时空中的非拓扑孤立子

讨论了反de Sitter时空中的非拓扑孤立子,利用渐近级数展开法,证明了在该时空中,只有势函数满足一定条件的非拓扑孤立子才能存在。     

q-Kampé de Fériet函数的简化和求和公式

利用著名的Jackson变换和Heine变换公式建立了几个一般形式的双变量q-级数变换公式。经过参数特殊化,推导出一系列关于q-Kampé de Fériet函数的简化与求和公式。     

First record of Monobrachium parasitum Mereschkowsky, 1877 (Cnidaria: Hydrozoa) from Brazil,in the tropical southwestern Atlantic,and its implication for bipolarity concepts

Project HABITATS was recently initiated in the Campos Basin (20.5–24° S), state of Rio de Janeiro, to assess biological impacts of petroleum exploration and exploitation on the continental shelf and slope of Brazil. Among species discovered in benthic samples from the area is the hydroid Monobrachium parasitum Mereschkowsky, 1877, occurring in an epibiotic association with the pelecypod mollusc Mendicula ferruginosa (Forbes, 1844): this is the first report of an association between the two species. Monobrachium parasitum has always been reported from cold waters, and is generally considered bipolar. This report records the occurrence of M. parasitum at lower latitudes, where they have seldom been collected: it is also the first account of this species from South America and the southwestern Atlantic. Moreover, the bathymetric distribution of M. parasitum is extended to a depth of 998 m. No previous records exist of hydroids from the Brazilian continental slope.     

de Sitter空间中常曲率的类空超曲面

研究de Sitter空间中具有常数量曲率的类空超曲面, 将Cheng-Yau的自共轭算子□作用在对称张量T上, 得到了这类超曲面关于第二基本形式模长平方的一个拼挤定理, 加强了已有的相应结果。     

三维Minkowski空间中的伪球面曲线

在三维Minkowski空间中讨论伪球面曲线.首先给出Minkowski空间中伪球面曲线的Frenet标架,然后利用伪球面曲线的特殊性,给出它的一种新的Frenet标架形式,并进一步研究了该曲线为一些特殊曲线的情况.