Mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice using microsatellite markers

Bulked segregant analysis (BSA) of a BC, population derived from Congguang 41A//Miyang 23/Congguang 41B was used to map the nuclear fertility restorer gene for Honglian (HL) cytoplasmic male sterility. One hundred and fifty-nine microsatellite primer pairs were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM258 produced polymorphic products. The nuclear fertility restorer gene for HL cytoplasmic male sterility was mapped on chromosome 10, 7.8cM from RM258. The restorer gene may be clustered on chromosome.     

Restorable variation of cytoplasmic type of male sterile line in rice byin vitro culture

Rice male sterile (MS) lines, IR66707A and IR69700A, which possess the cytoplasm of Oryza perennis and O. glumaepatula respectively, belong to the cytoplasmic type. Their sterility could be maintained but not be restored. By somatic cell culture of these two MS lines, 47 somaclones with 465 R₁ plants were obtained. All of the 465 R₁ plants were sterile in the spring season in Guangzhou. According to the expression of the R₁ plants and the level of similarity to their donor parents, they could be divided into three types. The plants of type Ⅰ were male sterile. The sterility of some somaclones of this type could be restored by the test crossing varieties or alternated to fertile by changes of some environmental conditions. The hybrid F₁ of test cross from the MS somaclones in type Ⅰ was fertile while the hybrid F₁ from the donor MS lines was still sterile. The R₁ plants of type Ⅱ were similar to the donor parents and also male sterile. The hybrid F₁ from all of the plants of type Ⅱ crossed to test variety were still sterile, so they did not possess restorability. For the somaclone of type Ⅲ, all of R₁ plants were sterile in both male and female organs. No seed was set in both conditions of self and cross pollination. The fact that the restorable variants obtained in the cytoplasmic type of MS lines of rice by in vitro culture reported here should be the first sample in somaclonal variation in plant kingdom.     

中国优秀男子乒乓球运动员前三板技术分析

运用文献资料法、录象观察法、数据统计法等方法对29届奥运会中中国三名优秀男子乒乓球运动员前三板技术进行分析比较,阐述乒乓球运动前三板技术的特征,从而为中国运动员进一步提高前三板技术提供参考依据。     

车架横梁成型模具凸模型面的精密加工

应用UG CAM进行自动编程,生成机床能识别的加工代码,通过在加工中心上对工件的一次定位、夹紧并自动加工,解决了车架横梁成型模具凸模型面加工精度难以保证的问题.     

几种消毒剂杀菌性能的比较试验

选用目前常用的含氯、季胺盐消毒剂,针对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌进行了最小抑菌浓度、最小杀菌浓度的测定及定量杀菌试验。结果表明含氯消毒剂（强力消毒灵）对3种细菌的杀菌效果最好;双链季胺盐消毒液（康唯消）对3种细菌的抑菌效果最好;双季胺碘消毒液（百毒杀）对3种细菌的抑菌、杀菌效果较差。     

Morphological diversity of habrobathynellids (Parabathynellidae,Bathynellacea) in India,with the description of a new species

A new species, Habrobathynella indica, is described. The male thoracopod VIII of this species is unique within the Parabathynellidae in its penis‐like extension of the internal lobe reaching thoracopod VII. The other two species known from India also have features unique to either the family or the genus. Habrobathynella schminkei has a seta on the uropod distally of the thick terminal spine of the spine row, a seta otherwise unknown in the family, and H. nagarjunai has a homonomous spine row on the uropod, which is lacking in other species of the genus.     

去势对成年雄性斑胸草雀LMAN神经元电生理特性的影响

以成年雄性斑胸草雀为研究对象,利用膜片钳技术观察去势前后LMAN（lateral magnocellular nucleus of the anterior neostriatum）的电生理活动,进一步探究AFP通路与鸣曲的关系. 实验分为正常组和去势组. 去势后,LMAN神经元的膜时间常数变短,膜电阻减小,动作电位潜伏期缩短,动作电位后超极化幅值变低同时动作电位发放频率变快. 结果表明,去势后LMAN神经元兴奋性增强,使AFP通路更加活跃,去势引起的鸣曲不稳定可能是AFP通路活动增强所致.     

淀粉酶类亏损与水稻三系雄性不育

本文以水稻三系及杂种的花药为材料,考察了淀粉代谢酶类亏损、恢复与雄性不育的相关作用。测验表明,不育系花药含淀粉0.5μg/spl(小穗花药共6枚),只达可育株系含量的1/12至1/10,花药含蛋白质15μg/spl,相当于1/7—1/5可育株系含量.对比分析各株系发育中花药的α-,β-淀粉酶,P-酶,R酶活力,得出P-酶活力为0.3μgPi/spl·h,α-淀粉酶活力为1.2 IDC/spl·h.恢复后的杂种代花药P-酶活力上升5—8倍,α-淀粉酶活力上升2.5-5倍.凝胶电泳证明。P-酶从单核期,α-淀粉酶Ⅱ从双核期开始恢复。比较3种恢复系在杂种代的恢复程序,得出其恢复力顺序为IR 244-6≥IR 24》铁陆17.这正好是花粉可育率恢复力顺序.这些事实足以表明不育性与淀粉合成有关酶的亏损相关,育性恢复是多因量变现象.     

温敏雄性核不育水稻几种同工酶的热激反应

在芽期、苗期和减数分裂期分别对温敏雄性核不育水稻培矮645、常规水稻湘晚籼2号进行38℃、25℃、22℃处理,分析其穿期、苗期和减数分裂期同工酶的变化,研究结果表明：温敏雄性核不育水稻培矮64S在热激条件下同工酶比常规水稻湘晚籼2号的变化大。热激条件下三种同工酶的变化,以细胞色素氧化酶最大,醋酶次之,过氧化物酶最小。     

致密双星系统的引力辐射计算

利用Landau-Lifshitz的能量-动量张量表述和四极矩公式对双星模型下的致密天体引力辐射源进行了讨论、计算了几种典型的致密双星系统的引力辐射(如PRS1913 16双星、PSRJ0737-3039A/B双脉冲星和双黑洞系统),并将计算结果与实际观测结果进行了比较,发现所得的结果在量级上与实际观测值在精度允许的范围内一致.说明双星模型和Landau-Lifshitz的能量-动量张量表述确为研究引力波辐射的一个好的近似.参13.