红莲型水稻不育系与保持系线粒体DNA酶切分析

为研究红莲型水稻细胞质雄性不育的分子基础，我们提取纯化了红莲型水稻丛广４１Ａ和丛广４１Ｂ的线粒体ＤＮＡ，采用限制性内切酶酶切片段长度多态性（ＲＦＬＰ）方法比较了红莲型不育系和保持系线粒体ＤＮＡ酶切图谱，发现两者之间存在一定的差异，部分支持了细胞质雄性不育与线粒体ＤＮＡ有关的结论。     

七星瓢虫雌成虫带精越冬及去雄饲养的研究

试验发现越冬代七星瓢虫雌成虫，春季饲养时不经雌雄交配，单雌可产卵，卵能正常孵化。结果证明：①春季去雄饲养单雌产卵量和卵的孵化率与雌雄交配混养无差异。②单雌所产的卵及子代各虫态历期和发育所要求环境条件与混养相同。③对越冬期雌成虫进行解剖镜检，证实储精囊有精子束，带精越冬。     

植物雄性不育的遗传机制探讨

本文评述了雄性不育性的遗传分类、细胞质育性因子、核质互作方式、环境调控、遗传操作等方面的研究进展，提出了解释雄性不育产生的双基因模型假说，认为控制雄性育性表达有二类核基因，一类是控制小孢子发生过程的基因（花粉发育结构基因），另一类是调控花纷发育结构基因表达条件的基因（调节基因），这二类基因任一发生突变均将产生遗传的雄性不育，细胞质（内环境）和生境（外环境）通过调控调节基因的表达成其产物，使得花纷发育结构基因表达所需条件不能满足而导致雄性不育．     

The male pheromone of the old house borerHylotrupes bajulus (L.) (Coleoptera: Cerambycidae): Identification and female response

We report here the identification of the long-range, male-produced sex pheromone of the Old house borerHylotrupes bajulus. Chemical analysis of hexane extracts obtained by surface extraction from dissected prothoracic glands and from headspace samples of the two sexes, revealed male-specific compounds: (3R)-3-hydroxy-2-hexanone, 2-hydroxy-3-hexanone, the diastereomeric diols (2R, 3R)-2,3-hexanediol and (2S, 3R)-2,3-hexanediol, 2,3-hexanedione, as well as 1-butanol.In wind tunnel bioassays we tested the influence of these male-specific compounds from the prothoracal glands on the behaviour of unmated and mated females. Specific behavioural sequences of the tested females (activity, running behaviour, searching, cleaning, flying, extension of ovipositor) were recorded. Unmated females were attracted by male beetles, headspace extracts of males, synthetic blends of the major pheromone compounds as well as by the components (3R)-3-hydroxy-2-hexanone, and the diastereomeric diols. Hexane, female beetles and 2,3-hexanedione did not attract unmated females. The reactions of mated females to male beetles and headspace samples did not differ significantly from those of the controls.The results of the bioassays show that the two-stage premating behaviour is initiated by emission of a long-range sex pheromone from the male prothoracal glands, which functions as an activator, attractant, and possibly aphrodisiac for unmated females.     

油菜染色体工程研究─—蓝花子特性导入油菜

利用染色体工程，合成油菜－蓝花子杂种双二倍体、倍半二倍体及油菜染色体异附加材料．油菜与蓝花子杂种双二倍体（ＡＡＣＣＲＲ）体细胞染色体数２ｎ＝５６，其植株开白花，ＰＭＣ＿ｓ减数分裂中期Ⅰ具有２８Ⅱ，雌雄育性都较差，倍半二倍体（ＡＡＣＣＲ）的２ｎ＝４７，开白花，ＰＭＣ＿ｓ不能完成减数分裂，雌雄性均不育．双二倍体育性受温度影响较大，１５～２５℃是它开花的最适温度，通过测交，发现异附加材料Ａｄ－６具有恢复油菜ＯｇｕＣＭＳ雄性育性的特性．提出并初步证实了“利用雄性不育的萝卜胞质作为选择压力，保存和纯化油菜ＯｇｕＣＭＳ恢复基因”育种思想的可行性．并证实了异附加材料Ａｄ－５具有雄性不育的遗传特性．     

番红花不育现象的观察

通过对北京地区栽培的番红花生殖器官的观察,发现它的雌配子体发育正常,花粉能在柱头上萌发,只因花粉管不能伸入到花柱和子房而不能受精。     

圈养雄獐的行为格局

于2003年12月到2004年3月,采用焦点动物取样和连续记录的方法,在浙江省嵊州市河麂种源繁育基地对成年雄獐和1岁龄雄獐在交配季节与非交配季节的行为格局进行了比较研究.结果表明：无论是在交配季节还是在非交配季节,成年雄獐与1岁龄雄獐的行为模式均无显著的差异（P〉0.05）;但与非交配季节相比,雄獐在交配季节移动时间显著增加（P〈0.05）,卧息时间明显减少（P〈0.05）,而冲突行为和尾阴探究行为显著增加（P〈0.05）.最后对圈养雄獐的饲养管理提出了建议.     

从生理解剖学角度看男大学生形体训练中的肌肉练习

在面向男大学生开设的形体训练中,应根据男性形体发育的年龄特征,根据人体运动系统的特点,以科学的态度和方法,因材施教;应结合男性形体美的标准,针对人体不同部位的肌肉的解剖学形态结构基础,进行力量和柔韧性的系统练习;应让男大学生掌握简便易行的肌肉锻炼方法,使其受益终身.     

中外优秀男子100米运动员分段速度的灰色关联分析及我国运动成绩的目标规划

以中外优秀男运动员的百米分段速度为基础，综合运用灰色系统理论进行比较分析，找出影响我国运动员运动成绩的主要原因，并建立了中短期我国运动员百米跑各分段速度的训练规划模型，为教练员科学制订训练计划提供参考。     

Heterotrimeric G protein participated in modulation of cytoplasmic calcium concentration in pollen cells

Cytoplasmic free calcium concentration([Ca2+]c) in pollen cells of Lilium daviddi is measured with confocal laser scanning microscopy to investigate the effect of heterotrimeric G protein (G protein) on [Ca2+]c and the possible signal transduction pathway of G protein triggering cellular calcium signal. After application, cholera toxin (CTX), an agonist of G protein, triggers a transient increase of [Ca2+]c in pollen cells, and evokes a spatial-temporal characteristic calcium dynamics; while pertussis toxin (PTX), a G protein antagonist, leads to the decrease of [Ca2+]c. Both L-type Ca2+ channel blocker verapamil and inhibitor of IP3 receptor heparin inhibit CTX-induced [Ca2+]c increase. The results show that G protein may play a role in the modulation of [Ca2+]c through enhancing the extracellular Ca2+ influx and releasing of Ca2+ from intracellular stores.