Association of different mobile elements to generate novel integrative elements

Among the more important problems in modern hospitals is the prevalence of bacterial pathogens expressing resistance to multiple antimicrobial agents. The frequency of multiresistance suggests mechanisms by which bacterial species can concentrate and efficiently exchange a variety of resistance determinants. Mechanisms by which this occurs include insertion of transposons within transposons, coalescence through the activity of insertion sequences and the employment of integrons. In some instances, more than one of these mechanisms is involved in creating large multiresistance genetic elements. The association of the elements with transferable elements or transposons may promote rapid dissemination among clinical strains, and create further opportunities for inclusion of additional resistance determinants.     

平均分排序法合理性分析

依 A rrow提出的选举问题的四条公理为准则 ,利用联合尺度条件 ,阐明目前流行的筛选平均分排序法 (即去掉一个最高分和一个最低分后 ,用余下的各分数的算术平均分作为待评人的最后得分 )是一种看似公正 ,实为错误的排序方法     

Quick trickle permutation based on quick trickle characteristic sequence

0　IntroductionAblockcipherwithoutdataextensionorexpressionisaper mutation .Thesecurityofablockcipherschemeiscorrela tivewiththe permutations.Quicktrickle permutation[1 ] canmakethedatadisorderedwhenactingatthedataandmakeallprobabledistancebetweentwoelementsappear,soithasgoodcryptographicpropertiesandcanbeusedintheblockcipherwhichrequireshighsecurity.Aprivatekeyblockcipherisapermutationinn dimensionvectorspaceoverF2 atthecontrolofakey[2 ,3] .Moreover,manyblockciphersusepermutationstoexecuteso…     

Notable diversity in hemoglobin expression patterns among species of the deep-sea clam, <Emphasis Type="Italic">Calyptogena</Emphasis>

The deep-sea clams Calyptogena nautilei and C. tsubasa, which live in the cold-seep area at a depth of 3570 m in the Nankai Trough, Japan, have abundant hemoglobins (Hbs) in erythrocytes, similar to other Calyptogena species. We determined the cDNA-derived amino acid sequences of Hbs from two Calyptogena species. C. tsubasa was found to contain two dimeric Hbs, Hb I consisting of 145 amino acid residues and Hb II with 137 residues, similar to known Hbs from C. soyoae and C. kaikoi. Sequence identity was over 90% among the orthologous chains of Calyptogena Hbs. On the other hand, surprisingly, C. nautilei contained two monomeric Hbs, Hb III containing 141 residues and Hb IV with 134 residues. In addition, Hbs III and IV showed only 33–42% sequence identity with Hbs I and II from other Calyptogena species. The distal (E7) histidine, one of the functionally important residues of the heme protein, is replaced by glutamine in all Hb chains of Calyptogena species. A phylogenetic analysis indicated that C. nautilei Hb III is closer to Hb I from other Calyptogena species. We suppose that a Hb gene was duplicated at least three times in an immediate ancestor of Calyptogena and, presumably depending on physiological conditions different Hb sets are being expressed: dimeric Hbs I and II in C. soyoae, C. kaikoi and C. tsubasa, and monomeric Hbs III and IV in C. nautilei. Received 13 May 2003; received after revision 5 June 2003; accepted 12 June 2003     

树的拓扑指标Z_1(G)

分子图的拓扑指标Z1(G)是反映分子物理和化学性质的重要拓扑指标,在n个顶点的树中具有最大Z1(G)和最小Z1(G)的树的基础上确定了n个顶点的树中具有次大,第三大及第四大Z1(G)的树.     

相关序列图像复原算法研究

针对气动光学效应降晰过程的复杂性,提出了一种基于图像相关性的两帧序列图像复原算法。该算法利用图像的相关性,结合气动光学效应降晰函数辨识,在大气流场的光学退化模型未知的情况下,采用两帧序列图像降晰函数辨识实现气动光学效应退化图像复原。为了克服降晰函数辨识算法可能带来的误差,在序列图像辨识降晰函数时,提出将辨识得到的两帧序列图像降晰函数结果差的二阶范数作为惩罚项,当该范数不符合要求时,根据序列图像的质心偏移和旋转偏移对图像进行相应的修正。给出了实现该方法的具体步骤,仿真结果证明了算法的有效性。     

Construction of an electronic physical map of Magnaporthe oryzae using genomic position-ready SSR markers

Magnaporthe oryzae is a model for plant pathogenic filamentous fungi. We have assembled a simple sequence repeat (SSR)-based physical map of the species, using in silico sequence data. A set of 120 SSR markers was developed from the genomic sequence of the reference isolate 70-15. These markers were readily amplified from the genomic DNA of other isolates, and high levels of allelic variation characterised the parental isolates of the two crosses tested. All the markers were locatable to one of the seven M. oryzae chromosomes. An SSR-based physical in silico map was constructed, and pre-existing SSR and RFLP loci were integrated into the map, along with 23 Avr (avirulence) genes and two other genes of importance to the plant/pathogen interaction. This map provides a platform for population genetics and functional genomics studies in the model pathogen, and even in other evolu- tionally related pathogens.     

联合光流技术和区域轮廓模型跟踪运动目标

研究了飞机在天空飞行图像中的飞机跟踪问题.在分析飞机在天空飞行图像特点的基础上,提出了联合光流技术和区域轮廓模型跟踪运动目标的方法.首先,对图像进行高通滤波预处理,提取一些高亮度的斑点,获得运动目标的特征图像;然后用特征图像求解运动目标的光流,以光流对运动区域进行分割,获得运动区域的中心和半径,以该中心和半径的圆作为水平集的初始曲线;再采用Song and Chan方法快速检测运动目标的边缘.实验证明,该方法能够快速、准确地自动跟踪运动目标.     

蜡状芽孢杆菌对番茄青枯雷尔氏菌致病性的影响

青枯雷尔氏菌（Ralstonia solanacearum）从其致病性上可分为能使植株发病的强致病力菌株和不能使植株发病的弱致病力菌株．利用蜡状芽孢杆菌（Bacillus cereus）与致病性稳定的青枯雷尔氏菌强致病力菌株进行共培养，处理后的青枯雷尔氏菌在TTC平板上表现为弱毒株的菌落特征，出现了致弱现象．经过16S rDNA基因序列测定，证明了用蜡状芽孢杆菌处理前后的菌株为青枯雷尔氏菌，并非是其它的污染菌株，通过回接番茄盆栽苗发现致弱前的青枯雷尔氏菌能有效引起番茄发生青枯病，而致弱后的菌株丧失致病力．不能引起番茄发病，     

五指山猪生长激素基因全序列克隆及序列分析

通过筛选一对引物，PCR扩增五指山小型猪GH基因全序列，并进行克隆测序分析、序列分析及氨基酸分析．结果表明，有98个位点不同，同源性为95．026％，外显子有2个碱基发生了突变，其中外显子2有1个位点的碱基突变导致了氨基酸异义取代。