Symmetrical directional cloning: An efficient method to prepare hairpin RNA interference constructs

RNA interference (RNAi) is a loss-of-function approach by which double-stranded RNA (dsRNA) initiates degradation of homologous mRNAs in a sequence specific manner. The dsRNA molecules can be produced in vitro or in vivo, and can be introduced to cells in a number of ways. Here we report a more efficient method for the cloning of inverted repeat DNA fragments into expression vectors that can be transcribed into effective dsRNA molecules in vivo or in vitro. This method, named Symmetrical Directional Cloning (SDC), takes the advantage of compatible non-palindromic restriction enezyme sites, which allow one to directionally clone a single PCR product in both the sense and antisense orientations together into a vector. SDC allows for the directional cloning of inverted repeats using a single PCR product; it requires only one cut site on each side of the loop. Hence this method is more cost effective and less time-consuming. At least 21 commercially available restriction endonucleases can be used as cloning sites for the SDC method. The efficacy of dsRNA expression vectors prepared by SDC has been demonstrated by targeting a negative regulator of the signaling pathway mediating the response of cells to phytohormone, gibberellins (GA), in the aleurone cells.     

扰动频率对动压气体轴承动态刚度和阻尼系数的影响

采用偏导数法求解动压气体润滑Reynolds方程，给出了动压气体轴承动态刚度和阻尼系数普遍适用的计算方法．进行了有限元数值仿真，计算了动压气体轴承的动态刚度和阻尼系数，并重点研究了扰动频率对这些系数的影响．计算结果表明，动压气体轴承的动态刚度系数随着扰动频率的增大而增大，动态阻尼系数随着扰动频率的增大而减小．从理论上解释了动压气体轴承的动态刚度和阻尼系数与扰动频率的密切相关性．     

一类广义凸集值映射优化问题弱有效解的最优性条件

在拓扑向量空间中定义了(u,0V)-广义次似凸集值映射.在相对内部的条件下,利用凸集分离定理,建立了此映射的择一定理.利用此择一定理,获得了带广义等式和不等式约束的优化问题的弱有效解的最优性条件.     

R~2中变形Helmholtz方程的某些边值问题

在空间物理,核物理,激光和电子技术等应用领域,有很多与Helmholtz方程以及变形Helmholtz方程相关的边值问题,但目前针对性的研究成果还很少.研究了变形Helmholtz方程的一类Riemann-Hilbert边值问题,利用复方程相关理论,讨论了在不同条件下其解的存在性和唯一性,并得出了可解性定理,利用获得的结果,讨论了单位圆G上的二阶变形Helmholtz方程的斜微商边值问题.     

方向提升小波变换域稀疏滤波的自然图像贝叶斯压缩感知

针对压缩感知(CS)重构算法在实际应用中自然图像的小波变换系数往往无法稀疏的问题,提出了一种方向提升小波变换(DLWT)域稀疏滤波的自然图像贝叶斯压缩感知算法(DLWT-SFTSW-BCS)。首先对自然图像进行方向提升小波变换得到小波变换系数;然后在随机测量之前利用稀疏滤波切除小系数,消除了小系数对大系数重构时的混叠干扰;最后结合小波树结构的贝叶斯压缩感知重构算法得到自然图像的重构图像。实验结果表明,与仅利用尺度间相关性的小波树结构的压缩感知重构算法相比,DLWT-SF-TSW-BCS算法的重构峰值信噪比最大可提高10dB。     

Towards a Wireless Sensor Platform for Energy Efficient Building Operation

Currently, the IT-support for energy performance rating of buildings is insufficient. So-called IT-platforms often "built" of an ad-hoc, inconsistent combination of off-the-shelf building management compo-nents, distributed data metering equipment and several monitoring software tools. A promising approach to achieve consistent, holistic performance data management is the implementation of an integrated, modular wireless sensor platform. This paper presents an approach of how wireless sensors can be seamlessly integrated into existing and future intelligent building management systems supporting improved building performance and diagnostics with an emphasis on energy management.     

电弧等离子体冶炼提纯工业硅(英文)

开发了一种把电弧等离子体冶炼和电磁感应搅拌熔体相联合的在高纯石墨坩埚内冶炼提纯工业硅的硅冶炼提纯技术.掺入到电弧等离子体工作气体流中的反应性气体在硅熔体液面处与杂质反应生成挥发性杂质化合物.工业硅中难去除的杂质是B,P和Al.用由水蒸气构成的反应性气体精炼熔融态工业硅来降低其中B和P的含量,随后定向凝固移除其他杂质.用此方法冶炼处理工业硅,其中的B,P,Al,Fe,Ti,Ca和Cu的含量从7.67,73,1931,2845,166,235和56mg/kg分别降低到5.85,29,202,676,36,89和9mg/kg.硅的纯度由原来的99.46%提纯高到99.93%.     

一类广义不变凸函数的最优性条件

通过在Banach空间上定义一类新的广义不变凸函数, 给出了这类函数的一些性质, 并在该类广义不变凸的条件下, 给出了目标函数是实值函数和向量函数的最优性必要条件及向量优化问题的充分必要条件.     

定向井的地质录井服务技术探讨

针对目前定向井给地质录井带来的挑战,就如何提高定向井,尤其是大位移定向井的录井质量,如何驾驭钻头沿最佳轨迹路线行驶,如何提高定向井的地质录井服务工作进行了探讨。     

时间分数阶电报方程的一种解技巧

提出了求解时间分数阶电报方程的一种计算有效的解技巧.我们考虑了带初边值条件的时间分数阶电报方程的解问题,借助于变量分离技巧和Adomian分解法,得到该问题分别在齐次和非齐次Dirichlet边界条件下的解析解和近似解,它们都可显式地表示成级数形式,从而易于近似数值计算.