JWA protein binds to α-tubulin in PC12 cells

Our previous study elucidated that JWA protein was a newly identified microtubule-associated protein (MAP), which combined to and co-localized with α-tubulin. In the present study, we designed a series of experiments to explore if any interactions between JWA protein and α-tubulin existed and how JWA protein would functionally link to α-tubulin, especially in cell mitosis. Results of coimmnnoprecipitation, gene transfection and immnnoflnores-cence microscopy from PC12 and HEK293 cells provided strong evidence for a linkage between JWA protein and α-tubulin. Our data showed that JWA protein bound to α-tubulin stably no matter whether α-tubulin was polymerized or not. In addition, by using antisense oligonncleotides, cell cycle blocking agents and hypothermia disposal techniques, we also found the interaction between JWA protein and α-tubulin. The further analysis using flow cytometry and confocal microscopy showed that both proteins co-existed in PC12 cells and were independent on the cell cycle. In conclusion, JWA protein is a newly identified microtnbnle-associated protein, binds to α-tubulin, and probably plays an important role in regulation of microtnbnlar stability.     

建筑空调复合节能技术初探

外墙热工性能对空调能耗有重要意义，空调排风所损失的能量也很可观，本文提出在建筑外墙的内部设置空气层作为空调系统排风通道，通过排风与墙体进行热交换，回收利用空调排风所带能量，可望在降低外墙传热造成的能耗损失、回收空调排风所带走的能量、降低室外环境温度变化与室内热舒适性之间相关性等三个方面获得益处。     

绿色包装的评价标准及环境标志

针对我国出口商品受阻于国际绿色贸易壁垒的现状,指出制定绿色包装的评价标准和环境标志势在必行;对绿色包装的内涵、科学评价方法、目前应实施的评价标准,以及绿色包装的环境标志进行了阐述和探讨;提出了对绿色包装实行分级,再根据分级标准用LCA(lifecycle aisessment)方法和可操作的5项指标分别制定评价标准,并相应地分别授予ISO14000Ⅰ型、Ⅲ型和单因素的环境标志的对策。     

碱减量印染废水生物处理剩余污泥脱水性能的试验研究

碱减量印染废水生物处理产生的剩余污泥具有很大的比阻值，脱水性能很差；污泥中含有的聚合物及其亲水性的水解产物可能是其大比阻的主要原因。加入污泥调理剂，污泥调理剂和污泥颗粒之间通过电性中和、改变固体颗粒周围化学环境等复杂的作用，可以改善污泥的脱水性能。     

蓄冷技术在空调系统中的应用分析

本文分析了蓄冷空调的国内背景、移峰潜力、经济和社会意义，介绍了几种常见冰蓄冷空调系统的运行模式，对冰蓄冷空调系统的经济性进行了分析．     

神经网络PID控制器在高大空间恒温空调系统中的应用

针对暖通空调领域中高大空间恒温空调系统大滞后、慢时变、非线性的特点以及不确定干扰因素多的实际情况，将具有自学习、自适应功能的神经网络PID控制器应用于空调系统中，并利用非线性辩识算法对控制过程进行预测输出，最后在空调房间数学模型的基础上通过Matlab环境下的计算机仿真和对某电视台大型演播室进行温度控制实验相结合，发现在高大空间恒温空调系统中神经网络PID控制响应快、超调小，验证了神经网络PID控制器的实用性和有效性．     

均质压燃的燃烧循环变动试验

为研究均质压燃的燃烧循环变动规律，在单缸发动机上进行了不同辛烷值燃料的均质压燃试验．结果表明，在恒定的平均指示压力下循环变动随辛烷值的增大而增大，在恒定的辛烷值下循环变动随平均指示压力的增大而减小；对于高辛烷值燃料，转速对循环变动的影响非常明显，尤其在较高转速时，循环变动随转速的升高急剧增大；在不发生爆震的情况下，燃烧效率、指示热效率以及HC和CO排放随循环变动的减小而改善，而NOx排放几乎没有变化，但一旦出现爆震情况，则指示热效率降低，NOx排放急剧升高．     

An integrative model and analysis of cell cycle in fission yeast

Cell cycle is a programmed process, during which a cell proliferates to two daughter cells. The eukaryotic or-ganisms share the same characters, such as four cycle phases G1, S, G2 and M, the evolutionally conserved cell cycle proteins and its dependent kinases, and the check-points mechanism[1,2]. Due to the different functions and the complicated interactions of these proteins involved in cell cycle, it is very difficult to understand the regulatory mechanism of cell cycle in a whole sense …