小麦耐盐突变体及其亲本在生育中后期生理指标的比较研究

对小麦耐盐突变体H870 6 - 34 ,RH870 6 - 49及其亲本濮农 36 6 5 ,百农 30 39在生育中后期非盐胁迫下的SOD活性进行了比较研究。观察其在不同生育时期不同材料间的变化情况发现 ,在小麦生育中后期 ,耐盐性较好的材料RH870 6 - 49SOD活性相对较高 ,细胞质膜透性相对较低。而耐盐性较差的材料H870 6 - 34的SOD活性相对较低 ,细胞质膜透性相对较高     

叶绿素缺乏大麦突变体叶绿体结构功能及生化特性的研究

突变大麦类囊体膜吸收光谱的蓝区峰值明显高于野生型;A683/A652比值较高;CD谱信号较弱,这说明其捕光色素蛋白复合物发生了变化。SDS-PAGE结果表明,突变大麦的Rubisco的水平与野生型一致,但在类囊体膜上分子量为24-30kD的LHCⅡ蛋白组分减少。     

Construction and characterization of partiallyntrC-deleted mutants inAlcaligenes faecalis

To study the effect of ntrC gene product on the expression and regulation of other important nitrogen-fixing genes in Alcaligenes faecalis, partially ntrC-deleted mutants of A. faecalis have been generated. To start with, the ntrC gene of A. faecalis was cloned into a suicide plasmid pSUP202 to create a recombinant plasmid pSUM1. The ntrC gene in pSUM1 was then replaced by a lacZ-Km^r fragment resulted in the generation of a plasmid pSUM2. The lacZ fragment in pSUM2 was further removed and a plasmid pSUM3 produced. As a second step, the plasmid pSUM2 or pSUM3 was introduced into the wild type of A. faecalis A1501 by conjugation and two partially ntrC-deleted mutants A15CM1 (ntrC∷lacZ) and A15CM2 (ntrC ^- ) were obtained. To understand the regulatory effect of the NtrC on the expression of nifH and nifA, a nifH-lacZ gene or a nifH-lacZ gene was introduced into the ntrC^- mutant by conjugation. The results indicated that: (ⅰ) although the ntrC^-mutant was nif ⁺ , its nitrogen fixation activity was only 20% that of the wild type; (ⅱ) the ntrC^- mutant failed to grow on the medium containing nitrate as a sole nitrogen source; (ⅲ) the regulation of ntrC gene expression did not require its own product; (ⅳ) the expression of nifH in A . faecalis was positively regulated by the ntrC. Deletion of the ntrC resulted in the reduction of nifH expression or even totally inactivated nitrogen fixation; (ⅴ) there was no obvious influence on the expression of nifA in A. faecalis if the ntrC gene was deleted.     

定点突变内皮抑素Zn2+的结合位点及突变基因的克隆表达

从人胚肝组织中提取总RNA, 以逆转录聚合酶链式反应（RT-PCR）法获得人内皮抑素编码序列, 采用定点突变技术将His2和His4双突变为Leu2和Val4. 将突变基因cDNA插入含有T7启动子的质粒pET-28b中构建表达质粒pMendo, 转化大肠杆菌BL21(DE3), 筛选表达菌株BL21-Mute, 表达菌株经IPTG诱导后以包涵体方式产生大量内皮抑素突变蛋白. SDS-PAGE分析表明, 表达的重组蛋白占菌株可溶性蛋白质的30%. 复性、 纯化的内皮抑素突变蛋白纯度达到98%, 失去抑制人脐静脉内皮细胞增殖的活性.     

基于ECGP的企业数据集成研究

提出了一种基于ECGP的数据集成模型,克服了传统数据集成模型中数据源节点过多带来的集成效率低、数据节点管理混乱等不足.ECGP提供了一种对等节点的分层方法,将节点按照拓扑优先级聚集成簇,从普通对等节点中选出主干节点作为簇的中心和服务提供者.还提出了基于软件即服务(SaaS)的NUTS-Sync数据集成框架,并讨论了2种数据集成模式:同步集成模式;异步集成模式.应用案例验证了模型的可行性和有效性.     

面向集群应用的太阳能中央热水工程控制器的设计与实现

针对面向集群应用的太阳能中央热水工程的功能需求和运行特点,提出了相应控制器的设计方案,实现了工程主要部件及其安装方式可配置的设计,解决了因不同安装环境、不同技术需求而产生的多种配置、安装方式的控制问题,改进了提高能源利用率的控制模式,实现了工程的现场和远程测控功能,为太阳能工程的集群应用和信息化管理提供技术支撑.     

The effect of a secretion-enhanced heavy chain on improving intein-based dual-vector co-delivery of a full-length factor VIII gene

Treatment of hemophilia A by gene therapy is adversely affected by inefficient FVIII secretion and the large FVIII gene, which is difficult to package in the promising adeno-associated virus (AAV) vectors. Inhibited secretion of FVIII is caused mainly by inefficient secretion of its heavy chain. Previously, we have employed a protein splicing-based dual-vector to co-transfer a B-domain-deleted FVIII (BDD-FVIII) gene, suggesting that the light chain, covalently ligated to a co-expressed heavy chain can improve the secretion of spliced BDD-FVIII. However, its level of secretion was affected by inefficient secretion the heavy chain. Here, we studied the effect of a mutant heavy chain with L303E/F309S substitutions, which enhance FVIII secretion on the heavy chain itself and spliced FVIII when using a protein splicing-based split-delivery of a full-length FVIII gene. Eukaryotic vectors expressing Ssp DnaB intein-fused mutant heavy and light chains were transiently co-transfected into cultured COS-7 cells. A spliced FVIII protein was seen in co-transfected cells by Western blot analysis. The heavy chain was secreted by cells transfected with the mutant heavy chain gene alone at (39±11) ng/mL and this secretion increased to (123±13) ng/mL when cells were co-transfected with the light chain gene, which was greater than the secretion of wild-type heavy chain. The amount of spliced FVIII in the culture supernatant of co-transfected cells was (86±14) ng/mL, with an activity of (0.61±0.08) IU/mL, which was greater than that of wild-type FVIII co-transfected cells. Spliced FVIII and bioactivity were also detected in the combined culture supernatant of cells individually transfected with mutant heavy and light chain gene at a higher level than that of combined wild-type heavy and light chain transfections. This suggested that the heavy chain with improved secretion markedly increased the efficacy of protein splicing-based split delivery of the full-length FVIII gene using a dual-vector. These results encourage the transfer of this technology to an animal model using a dual-AAV vector.     

大果水晶梨与其褐色突变体多酚氧化酶及过氧化物酶同工酶差异(简报)

大果水晶梨果皮为绿色,2007年在其植株上发现一果实呈褐色的变异枝,经2008-2009年嫁接试验后确定为遗传性变异。与原品种比较,褐色突变体果实多了一层褐色木栓层。     

A spontaneous benomyl-resistant mutant ofPodospora anserina exhibiting a diurnal growth rhythm

Summary A spontaneous mutant ofP. anserina isolated by screening for benomyl resistance exhibited a diurnal growth rhythm dependent on light-dark cycles. The rhythmic character, the benomyl resistance and a growth rate reduced to 50% of that of the wild type were inherited together over more than 10 generations. The locus was mapped on linkage group II, 0.35 map units distal to the locusz (=0.81 map units from the centromere).