全文获取类型
收费全文 | 18620篇 |
免费 | 635篇 |
国内免费 | 1087篇 |
专业分类
系统科学 | 560篇 |
丛书文集 | 706篇 |
教育与普及 | 88篇 |
理论与方法论 | 19篇 |
现状及发展 | 617篇 |
研究方法 | 1篇 |
综合类 | 18342篇 |
自然研究 | 9篇 |
出版年
2024年 | 25篇 |
2023年 | 83篇 |
2022年 | 151篇 |
2021年 | 216篇 |
2020年 | 197篇 |
2019年 | 183篇 |
2018年 | 186篇 |
2017年 | 261篇 |
2016年 | 314篇 |
2015年 | 436篇 |
2014年 | 679篇 |
2013年 | 566篇 |
2012年 | 892篇 |
2011年 | 1036篇 |
2010年 | 785篇 |
2009年 | 926篇 |
2008年 | 916篇 |
2007年 | 1178篇 |
2006年 | 1238篇 |
2005年 | 1068篇 |
2004年 | 947篇 |
2003年 | 939篇 |
2002年 | 845篇 |
2001年 | 727篇 |
2000年 | 697篇 |
1999年 | 661篇 |
1998年 | 472篇 |
1997年 | 483篇 |
1996年 | 471篇 |
1995年 | 398篇 |
1994年 | 442篇 |
1993年 | 347篇 |
1992年 | 308篇 |
1991年 | 306篇 |
1990年 | 273篇 |
1989年 | 223篇 |
1988年 | 210篇 |
1987年 | 112篇 |
1986年 | 68篇 |
1985年 | 56篇 |
1984年 | 15篇 |
1981年 | 3篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
该文介绍了在Windowe3.1环境下.利用MicrosoftC(++)及其基本类库(MFC)开发光伏水泵报价系统的方法。该方法采用了面向对象技术,使软件具备可重用性。同时,MFC类库的使用使复杂的Win-dows应用程序的编程接口变得更容易。该方法对其它工程报价问题也具借鉴意义。 相似文献
102.
尹建军 《兰州理工大学学报》1995,(1)
以甲基异丁基甲酮(MIBK)-甲酸(FA)为流动相,对一些无机离子在硅胶薄层上的色谱行为进行了研究,得出了无机离子的R_F值与流动相中强极性溶剂甲酸的体积百分浓度C_p之间的关系以及所用流动相的溶剂性质参数B值.利用该流动相体系对部分多元离子混合液进行了分离. 相似文献
103.
Induction of hemopoietic chimerism in the caprine fetus by intraperitoneal injection of fetal liver cells 总被引:1,自引:0,他引:1
R. D. Pearce D. Kiehm D. T. Armstrong P. B. Little J. W. Callahan L. R. Klunder J. T. R. Clarke 《Cellular and molecular life sciences : CMLS》1989,45(3):307-308
Summary Intraperitoneal injection of allogeneic liver cells from 43-day-old male fetuses into normal 60-day female goat fetuses resulted in persistent hemopoietic chimerism in surviving recipients without clinical evidence of graft-versus-host disease. Transplantation of normal fetal liver cells into preimmunocompetent goat fetuses affected with -D-mannosidosis may provide an alternative strategy for evaluating hemopoietic stem cell transplantation in the treatment of human lysosomal storage diseases. 相似文献
104.
T. Hatano Y. Kato M. Katayama S. Marumo 《Cellular and molecular life sciences : CMLS》1989,45(4):400-402
Summary A new potent antiauxin, -(5,7-dichloroindole-3-)isobutyric acid has been synthesized and shown to inhibit auxin-mediated elongation ofAvena coleoptiles and to stimulate root growth of rice seedlings. Its activity is stronger than -(p-chlorophenoxy)isobutyric acid and is comparable to that of 2,3,5-triiodobenzoic acid, which are typical antiauxins. 相似文献
105.
A number of neuropeptides were isolated from the ganglia and muscles of molluscs, and their actions were examined. Diverse neuropeptides, in addition to several classical neurotransmitters, were suggested to be involved in the regulation of the anterior byssus retractor muscle ofMytilus. A wide structural variety of members of theMytilus inhibitory peptide family was observed in each of the generaMytilus, Achatina andHelix. Gly-Trp-NH2, the C-terminal dipeptide fragment of the neuropeptide AGPWamide, showed a more potent action than the parent peptide in all of the muscles examined. Peptides related to some molluscan neuropeptides were found to be distributed interphyletically. Some neuropeptides containing ad-amino acid residue were found inAchatina andMytilus. These aspects of molluscan neuropeptides are thought not to be exceptional. 相似文献
106.
We demonstrate for the first time a hair cycle-dependent gene and protein expression of proopiomelanocortin in mouse skin in vivo. Northern blot detected POMC mRNA with an apparent size of 0.9 kb in anagen but not telogen skin. Western blot emphasized a specific protein of 30–33 kDa recognized by anti -endorphin in late but not early anagen or telogen skin. By immunocytochemistry, -endorphin antigen was localized in the sebaceous gland in a hair cycle dependent manner. 相似文献
107.
In 1986, Brown and Clemmons (Proc. natl Acad. Sci. USA83 (1986) 3321) showed that platelets contain a substance, platelet-derived growth inhibitor (PDGI), that inhibits in vitro endothelial cell replication. Although platelets are rich in transforming grwoth factor (TGF-), PDGI was considered not to be related to TGF-, on the basis of its reported properties (extraction from platelets at neutral pH, binding to heparin-Sepharose). However, we purified PDGI to near homogeneity and showed that on the basis of HPLC retention behavior, in vitro growth inhibitory activities with several cell types, receptor binding, and immunoneutralization of growth inhibitory activity with specific anti-TGF- type 1 antibodies, PDGI is most probably identical with TGF- type 1. 相似文献
108.
D. Porquet M. Appel T. Fournier O. Bertaux D. Biou J. Féger 《Cellular and molecular life sciences : CMLS》1992,48(3):257-261
Both in vivo and in vitro models have certain disadvantages for the study of the chronic hepatotoxicity of drugs. The aim of this work was to evaluate a new approach based on an in vivo/in vitro model. After chronic in vivo treatment of rats with Vincamine and Vindeburnol (an eburnamenine derivative which exhibits hepatotoxic properties in man) liver cells were isolated, and functional and metabolic disorders (metabolic utilization of fructose and protein biosynthesis) were studied to determine injury. The results showed no modification of blood parameters, but a direct relationship between the dose of Vindeburnol administered in vivo and the metabolic disorders observed in vitro, evidencing the high sensitivity and reliability of this model. 相似文献
109.
R. Wiesendanger B. Martinoni T. Boller D. Arigoni 《Cellular and molecular life sciences : CMLS》1986,42(2):207-209
Summary Under the action of the appropriate synthase from ripe tomatoes a 11 mixture of (3S, 4R)-[3,4-2H2] and (3R, 4S)-[3,4-2H2]-(2S)-adenosylmethionine is transformed into a 11 mixture of the two meso forms of [2H2]-1-aminocyclopropanecarboxylic acid, a result which proves the operation of an inversion mechanism and which is consistent with direct nucleophilic displacement of the leaving group in the substrate. 相似文献
110.
N. Akaike K. Hattori Y. Oomura D. O. Carpenter 《Cellular and molecular life sciences : CMLS》1985,41(1):70-71
Summary Using isolated, internally perfused bullfrog dorsal root ganglion cells we have studied the dose-response curves for -aminobutyric acid (GABA) in the presence of internally or externally applied GABA antagonists. With external application of antagonists the inhibition of the GABA current by bicuculline was competitive and that by picrotoxin was noncompetitive. Picrotoxin but not bicuculline blocked when internally perfused.To whom reprint requests should be addressed.Acknowledgments. We thank Drs S. Minakami and S. Yasui for helpful discussions and comments. 相似文献