免疫鸡群新城疫病毒检测和分离的研究

以ＳＰＦ鸡抗新城疫病毒（ＮＤＶ）ＩｇＧ为包被抗体（５μｇ／ｍＬ）,兔抗ＮＤＶＶｅｒｏ细胞培养纯化毒ＩｇＧ为第二抗体（１∶２００～６００）,酶标记羊抗兔ＩｇＧ（１∶５０００）为指示抗体建立间接夹心ＥＬＩＳＡ,检测实验免疫鸡、攻毒鸡及现地免疫鸡口腔或泄殖腔外分泌物。结果表明：新城疫无毒力Ｖ４疫苗免疫后２ｄ,口腔内病毒抗原随机检出率为４／１０,第１０天为１０／１０;泄殖腔为０～１／１０,并持续１８ｄ以上。滴鼻攻毒后１ｄ,口腔和泄殖腔内病毒抗原检出率分别是８／１０和６／１０;第１３天,泄殖腔内病毒抗原检出阴性。ＮＤＶ灭活疫苗免疫６０ｄ后攻毒,第２天有７／２０口腔和８／２０泄殖腔检出阳性,并一直到攻毒后第１４天。非免疫对照鸡攻毒后第３天直到死亡时,口腔和泄殖腔均可检出病毒抗原。对各地临床上无任何症状的ＮＤＶ免疫鸡群检测发现,泄殖腔ＮＤＶ抗原阳性率０％～９．３％,分离到８株ＮＤＶ流行毒株,生物学试验证实这些毒株的毒力属中等毒力偏强或强毒。     

脆江蓠营养细胞超微结构的研究

脆江蓠营养细胞超微结构观察结果表明：营养细胞每一色素体内含有一条围周类囊体、４～１０条平行类囊体和一些质体小球．平行类囊体数目与细胞所处的部位有关．内质网呈环形片层结构．同心圆膜状体具有１或２个核心．色素体在末端进行分裂,分裂时平行类囊体数目增多．幼色素体被膜直接内陷形成平行类囊体．以后发育出围周类囊．     

螺旋藻藻蓝蛋白和藻多糖对人体外周血淋巴细胞功能的影响

从螺旋藻中提取藻蓝蛋白（ＳＰＣ）和多糖（ＳＰＳ）,研究其对人体外周血淋巴细胞功能的影响。实验结果表明,ＳＰＣ和ＳＰＳ能够促进ＰＨＡ刺激淋巴细胞转化的作用,其中ＳＰＳ的促进作用有剂量依存关系。ＳＰＣ和ＳＰＳ能恢复Ｔ细胞受环磷酰胺损伤后Ｅ玫瑰花环形成能力,特别是对活性Ｅ花环（Ｅａ）的形成能力有较好的恢复作用。     

MJ对抗旱性不同的两个玉米品种茎和根生长的影响

比较了ＭＪ对两个不抗旱性玉米品种的影响．１－１００ｍｇ／ＬＭＪ对抗旱性较弱品种ＳＣ７０１幼苗株高有抑制效应。     

2-Carboxyethylgermanium sesquioxide,a synthetic organogermanium compound,as an inducer of contrasuppressor T cells

2-Carboxyethylgermanium sesquioxide (Ge-132), a synthesized organogermanium compound with immunomodulaing activities, was shown to be an inducer of anti-suppressor T cells in normal mice. The suppressor cell activity of T6S cells, a clone of burn-induced CD8⁺ IL-4-producing suppressor T cells, was clearly inhibited when a mixed lymphocyte-tumor cell reaction of the clone was conducted with splenic mononuclear cells from mice treated orally with a 100 mg/kg dose of Ge-132. The activity of anti-suppressor cells was demonstrated in spleens of mice 2 days after treatment with Ge-132 and reached its peak on day 3. The anti-suppressor cells induced by the compound were of a contrasuppressor T cell-linage, because they were characterized as CD4⁺ CD28⁺ TCR/⁺ Vicia villosa lectin-adherent T cells. These cells produced IFN- but did not produce IL-2, IL-4, IL-6 or IL-10 in their culture fluids. CD4⁺ anti-suppressor T cells induced by Ge-132 may be different from other subsets of CD4⁺ T cells because Th1 and Th2 cells generated in our laboratory did not adhere toVicia villosa lectin-coated petri dishes, and each produced specific cytokines. Th1 cells produced IFN- and IL-2 while Th2 cells produce IL-4 and IL-10 in vitro. These results suggest that Ge-132 may be useful as an inducer of contrasuppressor T cells in immunocompromised individuals bearing suppressor T cells. To eliminate suppressor T cells from immunocompromised hosts may result in improved resistance from various opportunistic infections.     

‘Chloride-cell’ — like mitochondria-rich cells of salamander larva gill epithelium

Summary Two types of mitochondria-rich cells (MRC) are described ultrastructurally in the gill epithelium of salamander larva. They resemble MRC found in larval ventral epidermis. Histochemical localization of carbonic anhydrase indicated numerous positive reacting cells, most of them flask-shaped. Morphological and functional similarities to fish chloride cells are discussed.     

中药柴胡提取物对人肝癌细胞BEL-7402细胞内VCR蓄积的影响

目的　测定中药柴胡(BupleurunChineseDC,BCDC)提取物对人肝癌细胞BEL 7402细胞内VCR蓄积的影响,以探索柴胡逆转BEL 7402细胞多药耐药的机制.方法　高效液相色谱法测定BCDC作用于BEL 7402后细胞内VCR蓄积情况的变化.结果　柴胡可使人肝癌细胞BEL 7402细胞内VCR浓度升高(P<0.01).结论　柴胡可以增加VCR在BEL 7402细胞内的积聚浓度,部分逆转BEL 7402细胞的MDR.     

骨髓干细胞与急性心肌梗死的血管新生

骨髓干细胞可分化为心肌细胞、血管内皮细胞、平滑肌细胞等，能再生心肌和血管，可用于心肌梗死的治疗。本文就骨髓干细胞在血管新生方面的生物学特性及其对心梗后的血管新生作用作一综述。     

龟鳖类血细胞研究的进展

文章概述了龟鳖类血细胞——红细胞、有粒细胞(包括嗜酸性粒细胞、嗜喊性粒细胞、嗜天青粒细胞和嗜中性粒细胞)、淋巴细胞、单核细胞、血栓细胞的特性,显微和超微结构特点,血细胞发生及功能等方面的研究进展.