磷酸饥饿诱导大肠杆菌同步化

用改良的Ｋｅｐｅｓ磷酸饥饿法诱导大肠杆菌Ｋ—１２ＡＭ１２６４同步化生长，细胞经８轮同步化步骤后可在磷酸不限制培养基中自由生长保持２～３次同步化细胞周期。Ｋ—１２ＡＭ１２６４大肠杆菌的细胞增倍和分化周期分别为５５和１５ｍｉｎ，同步化细胞周期可分达３个时相。细胞分裂期（Ｐ），细胞分裂和染色体复制起始间期（Ｑ）以及染色体复制起始和细胞分裂间期（Ｒ）。Ｒ期又可分Ｒ１和Ｒ２两个亚期。在Ｒ１亚期胸腺呼啶掺入ＤＮＡ的速度增加，在Ｒ２亚期掺入速度保持恒定。Ｒ１和Ｒ２期分别为１５和１０ｍｉｎ。     

以人为本 依法治校——对实施新《规定》的几点认识与思考

新规定是高校学生管理与服务的政策依据,高等学校与学生的关系是一种特定的权利义务关系。该文围绕以人为本与依法治校的要求,结合学生工作的实际,对实施好新规定谈几点浅见。     

腐蚀电池法强化后续生化工艺处理印染废水

从腐蚀电池反应的基本原理出发,以腐蚀电池反应中的溶铁量作为外部监控指标,来探求腐蚀电池反应的最佳工艺条件。以循序间歇式活性污泥法(SequencingBatchReactor,SBR)为主体处理方法,在对比SBR法和腐蚀电池法-SBR法处理印染废水降解效果的基础上,重点考察了腐蚀电池对后续生化工艺的影响。研究表明,腐蚀电池法不仅提高了废水的可生化性,而且强化了后续生物处理中的活性污泥,使得腐蚀电池-SBR法在COD、色度去除率上明显高于SBR法。     

Programmed cell death during terminal bud senescence in a sympodial branching tree, Eucommia ulmoides

Eucommia ulmoides Oliv. is a typical sympodial branching tree. The apical bud of the branch ages and dies every year, replaced by the nearby axillary bud in the second year. Structural assays and a series of biochemical analyses were performed to analyze the senescence mechanism in the apical bud. It was revealed that most cells of the apical bud underwent the programmed cell death (PCD) during the senescence: the chromosomes were congregated and the nuclear contents were condensed, as shown by 4′,6-diamidino-2-phenylindole (DAPI) fluorescence. DNA fragmentation was detected during senescence using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling (TUNEL) method, coincident with the appearance of a DNA ladder. Moreover, a 20 kD DNase related to fragmentation was found. PCD was initiated first in the young leaves, leaf primordia and peripheral zone cells, then in the central mother cells and initial layer cells in the apical meristem. The terminal buds remain in vegetative growth during senescence, in contrast to buds of many annual plants.     

蒜氨酸增强昆明小鼠免疫调节功能的研究

以昆明小鼠为动物模型，用蒜氨酸溶液连续灌胃30d，观察蒜氨酸对昆明小鼠免疫调节功能的影响．结果显示，蒜氨酸能显著提高小鼠细胞免疫功能、体液免疫功能和巨噬细胞吞噬功能．     

黄土高原产业结构调整与生态产业建设对策

文章从黄土高原产业结构现状出发，根据生态经济学原理，提出产业结构调整与生态产业建设的重点与对策．     

Advances in Biological Water-saving Research:Challenge and Perspectives

Increasing the efficiency of water use by crops continues to escalate as a topic of concern because drought is a restrictive environmental factor for crop productivity worldwide.Greater yield per unit rainfall is one of the most important challenges in wa     

《道德经》对高等学校管理的启示

本文从无为而治、人本管理、柔性管理、创造安定团结的学校环境、领导者的个人能力和修养五个方面分析《道德经》对高等学校管理的借鉴作用。     

聚苯胺－碳复合电极材料研究

采用吸附聚合法制备聚苯胺－碳复合粉料，用涂覆法制备大面积、可卷绕的聚苯胺－碳－聚偏氟乙烯复合阴极膜作为锂二次电池的阴极活性材料．研究了试验电池的工艺特点和放电特性．     

蛋白激酶C单克隆抗体的制备

应用杂交瘤技术制备了蛋白激酶C(PKC)的单克隆抗体,用蛋白A-Sepharose-CL4B协同沉淀复合物分析单抗识别的蛋白,分子质量与PKC相同。采用该抗体对正常和转化的C_3H_(10) T_(1/2)细胞进行免疫荧光观察,发现它们的PKC含量明显不同。但荧光分布都主要集中在细胞质和细胞膜部分。