Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells

Summary Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG₁ antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascitec fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody.     

Selective protection of cells against X-irradiation. Isoproterenol protects only those cells that possesβ-adrenoreceptors

Summary In mixed culture of Chinese hamster fibroblatst, clone 431, and transformed murine L fibroblasts, clone B-82, isoproterenol was found to protect only 431 cells against ionizing radiation. It was shown that 431 cells, in contrast to B-82 cells, possess-adrenoreceptors, and the radioprotective effect of isoproterenol can be realized only if this agent interacts with-adrenoreceptors coupled with the cAMP system. Since malignization often causes the disappearance of-adrenergic and other hormone receptors, the combined culturing and irradiation of the cells studied can be regarded as a model of the growth of malignant cells (B-82) among normal tissue cells (431 cells) under conditions of radiation therapy. A possibility of selective protection against radiation damage of normal tissue cells, with retention of the former radiosensitivity of tumor cells, is discussed.     

宗教文化对泉州古城街道活力的影响分析

以泉州古城为例,利用多源数据构建街道活力与其影响因素的分析框架,建立包含街道物理环境影响因素和宗教文化影响因素的街道活力评价指标体系.分析宗教建筑对街道活力的影响,并进一步分析宗教建筑与街道的空间关系对街道活力的影响.研究结果表明：街道活力与街道两侧的寺庙宗祠的数量呈负相关,香火旺盛的寺庙宗祠会提升附近街道的活力,但与寺庙宗祠距离无显著相关关系;在寺庙宗祠入口设置广场或宽敞的步行区可以集聚人气,并在寺庙宗祠周围布置商业或与其他景点结合以形成连续的活力点,有助于提升街道活力.     

Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells^1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently.     

词汇中的文化蕴含

运用文化语言学的理论,从汉语词汇的意义、结构和交际三个方面进行论证,提出词汇与文化具有“融合关系”、“渗透关系”和“制约关系”。这些关系说明文化对词汇的理解、运用和规范有着很大的影响,重视文化因素对全面灵活地理解词汇的意义、深入细致地了解词汇的结构、准确扎实地掌握词汇的运用技巧大有裨益。每一语言系统中有相当一部分词汇的内涵是以民族文化为依托,文化是这些词义产生、发展、衍变的关键。在汉语词汇系统中,表义的结构形式反映了汉民族的思维方式、特点,是它们形成了汉语词汇的构成规则。传统的伦理等级观念、约定俗成的民族表达习惯附加给词汇各种文化色彩的语用意义,这些多维的复合性文化使开放的词汇系统变得有序可循。     

建立人输卵管上皮细胞无血清培养

人输卵管上皮细胞（HOEC）可以体外较长时间无血清培养,DMEM／F12培养液的培养效果优于Ham’sF10,在加有多种因子和胎球蛋白的培养液中,HOEC可贴壁生长,生长能力达到合质量分数15％FCS的对照组的30％～40％,一般可传代2次,最长培养可维持38d,细胞形态与有血清培养差别不大,虽然无血清培养HOEC的生长速度、密度、传代成功率远不及有血清培养,但能抑制成纤维细胞生长,维持较纯的上皮细胞。     

Marked electrical synapses on AP neuron of the leech (Whitmania pigra)

An initial attempt reveals parameters of marked electric synapse on AP perceptive neuron of leech. The width of gap between marked AP and either neuro-or glio-process is 2–3 nm, the diameter of the hexagonal array of connexion is 8–9 nm with a central pole of 2–2.5 nm, the same as the structure of annular lamellar bodies internalized by AP-glia processes in the AP.     

Endothelial Cell Culture on Different Phospholipid Surfaces *

ＩｎｔｒｏｄｕｃｔｉｏｎＢｉｏｍａｔｅｒｉａｌｓａｒｅａｌｗａｙｓｕｓｅｄｉｎａｎｅｎｖｉｒｏｎｍｅｎｔａｄｊａｃｅｎｔｔｏｌｉｖｉｎｇｔｉｓｓｕｅｓａｎｄｂｌｏｏｄ．Ｃｅｌｕｌａｒｉｎｔｅｒａｃｔｉｏｎｓｗｉｔｈｉｍｐｌａｎｔｓａｒｅｏｆｐｒｉｍａ...     

Construction of High Level Expression Cell Lines of Erythropoietin *

Ｅｒｙｔｈｒｏｐｏｉｅｔｉｎ（ＥＰＯ）ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｒｅｇｕｌａｔｏｒｙｆａｃｔｏｒｓｄｕｒｉｎｇｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｍａｍｍａｌｉａｎｅｒｙｔｈｒｏｃｙｔｅ,ａｃｔｉｎｇｍａｉｎｌｙｏｎｔｈｅｅｒｙｔｈｒ...