Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

Mechanical Studies on Treatment of Malignant Tumour by Ultralow Frequency Pulsed-Gradient Magnetic Fields

Ultralow frequency (ULF) pulsed-gradient magnetic field (with the maximum intensity of 0.6-2.0 T, gradient of 10-100 T·m-1, pulse width of 20-200 ms and frequency of 0. 16-1. 34 Hz) treatment of mice can inhibit murine malignant tumour growth and can induce apoptosis of cancer cell. The apoptotic cancer cell contracted, became rounder and divorced from adjacent cells; the heterochromatin condensed and coagulated together along the inner side of the nuclear membrane; the endoplasmic reticulums expanded and fused with the cellular membrane; many apoptotic bodies which were packed by the cellular membrane appeared and were devoured by some lymphocytes and plasma. By Lorentz force the magnetic field keeps the moving ions within bounds of Larmor radius. Thus, penetrating capability of the positive and negative ions through the cell membrane was affected, even the role on the cell membrane formed.     

环境、族群与文化——依山依水族群文化与社会发展研讨会主题讲演

所谓“依山依水”的研讨不是一种地理环境决定论的观点 ,而是要把它看成是人如何利用文化与环境调适互动的系统机制去探讨。在思考人与自然的关系时 ,人类学家要思考人与文化的关系 ,特别是文化怎样在人与环境之间互动 ,进而影响各族群的生计方式、社会组织和宗教信仰等等而形成体系的过程。     

地域文化视角与现代文学研究

真正从地域文化的角度来研究现代文学 ,是从 2 0世纪 90年代开始的。大量地区性的现代文学史料的整理出版 ,不仅为这种研究提供了可能 ,而且本身就包含着研究的眼光。《二十世纪中国文学与区域文化丛书》集中体现了这方面的研究成果。但总的说来 ,从地域文化的角度研究现代文学的努力 ,仍未摆脱建构现代性大叙事的诱惑 ,其研究潜力因此受到了根本性的限制。将地域文化视角转化为对话性的解构力量 ,反思现代性大叙事对差异性的压抑 ,是激活其研究潜力的关键 ,目前已有一些这方面的研究成果出现。     

高校英语教学中文化知识的处理

化不仅影响人们是否能够有效地学习语言，而且也影响着跨化交际中信息的传递与交流。因此在教授英语这门语言的同时，应重视对英语国家化的讲解。     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

心灵废墟——师陀早期创作的精神特质喻晓薇

作为现代文学史上一位具有独特风格和追求的作家,师陀早期创作中废墟意象高度密集,而这正是师陀独特的精神气质———废墟心理的外化。这种心理来自于其幼年时代形成的创伤性体验。这种心理特质体现为对现实中废墟的批判、对文化废墟的检省、对生命废墟的咏叹。这种独有的废墟精神特质使师陀在现代文坛优秀作家中占有一席之地。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

谈中国武术的节奏与文化

中国武术的节奏是鉴别不同拳种和套路的重要因素。文章从几个方面探讨了中国武术的节奏特点，并以大量的文史记载及拳理、拳论，阐明中国武术的节奏与传统哲学、文化的历史渊源。     

轿车车头道路模拟试验加载谱研究

结合实际工作需要，通过远程参数控制技术在6通道多轴随机振动台上开发了轿车车头内零部件的室内道路模拟试验技术，正确、快速地模拟试件在试车场强化道路上的振动特性，缩短了试验周期，降低了试验费用，建立了制定试验标准的基本流程。