虾青素的抗肿瘤作用及其机制

通过论述虾青素的抗肿瘤作用及机制研究,旨在为临床肿瘤治疗相关研究及未来应用提供参考。     

溶血磷脂酸受体2调控胃癌细胞SGC-7901侵袭迁移以及增殖凋亡

为探讨LPA2是否可调节胃癌细胞SGC-7901的侵袭迁移及增殖凋亡,将LPA2 siRNA和pc DNA3.1-LPA2表达载体转染SGC-7901,转染后利用Western blotting检测LPA2、E-cadherin及Vimentin蛋白表达情况,Transwell实验检测细胞侵袭迁移能力,MTS实验检测细胞增殖,流式细胞仪检测凋亡情况。结果表明LPA2 siRNA对胃癌细胞SGC-7901敲除成功后,细胞内的E-cadherin表达增高,Vimentin表达降低;同时其侵袭、迁移和增殖能力降低,凋亡能力升高。细胞SGC-7901过表达LPA2后,细胞内的E-cadherin表达降低,Vimentin表达增加,其侵袭、迁移和增殖能力升高,凋亡能力降低。可见LPA2可调控胃癌细胞SGC-7901侵袭迁移以及增殖凋亡,为胃癌的治疗提供新的靶点。     

人生长激素诱导体外培养的大肠癌细胞株细胞周期动？…

研究人生长激素（ｈＧＨ）对大肠癌细胞增殖和分化的影响，收集人大肠癌细胞株Ｌｏｖｏ和ＬＳ－１７４－Ｔ（简称１７４），以顺铂和／或不同浓度的ｈＧＨ体外培养细胞，２４ｈ后以流式细胞仪测定细胞增殖周期等指标。结果显示ｈＧＨ能诱导１７４细胞Ｓ期数率非常显著地增高（Ｐ〈０．０１）；Ｌｏｖｏ细胞１００ｎｇ／ｍＬ组Ｇ０－Ｇ１期参数下降（Ｐ〈０．０５），Ｓ期参数增加（Ｐ〈０．０１），２００ｎｇ／ｍＬ组Ｇ２－Ｍ期百分     

Advances in tissue engineering

Tissue engineering is a newly developed specialty involved in the construction of tissues and organs either in vitro or in vivo. Tremendous progress has been achieved over the past decade in tisse construction as well as in other related areas, such as bone marrow stromal cells, embryonic stem cells and tissue progenitor cells. In our laboratory, tissues of full-thickness skin, bone, cartilage and tendon have been successfully engineered, and the engineered tissues have repaired full-thickness skin wound, cranial bone defects, articular cartilage defects and tendon defects in animals. In basic research areas, bone marrow stromal cells have been induced and transformed into osteoblasts and chondrocytes in vitro. Mouse embryo stem cell lines we established have differentiated into neuron precursor, cardiac muscle cells and epithelial cells. Genetic modifications of seed cells for promoting cell proliferation, delaying cell aging and inducing immune tolerance have also been investigated.     

Differentiation and adaptation epigenetic networks: Translational research in gastric carcinogenesis

There are several kinds of epigenetic networks in the human body including the cell differentiation epigenetic network(DiEN) and the host adaptation epigenetic network(AdEN).DiEN networks are static and cell/tissue-specific.AdEN networks are variable and dependent upon environmental factors.DiEN and AdEN alterations can respectively serve as biomarkers for different kinds of diseases.Cancer is a consequence of accumulated pathophysiological adaptations of tissue stem cells to exposure of environmental carcinogens.Cancer cells are de-differentiated cells that obtain the capacity of unrestricted proliferation,local invasion,and distant migration/metastasis.Both DiEN and AdEN changes can be observed in cancer tissues.Alterations of DNA methylation are the most stable epigenetic modifications and can be sensitively detected in a small cell population.These advantages make DNA methylation the optimal biomarkers for detection of initiated cells in precancerous lesions and metastasis stem cells in cancer tissues.It has been proven that p16 methylation can be used as a diagnostic biomarker to determine malignant potential of epithelium dysplasia in many organs including the stomach.In a large-scale validation study on the DNA methylome of gastric carcinomas(GC),the methylation status of more than 90 CpG islands has been analyzed by DHPLC.Furthermore,GFRA1 demethylation and methylation of SRF and ZNF382 are frequent events during gastric carcinogenesis and consistently correlate to GC metastasis and overall survival of GC patients from China,Japan,and Korea,respectively.In a population study,it has been demonstrated that gradual increasing of plasma miR-211 and other miRNA levels may be an early risk predictor for GC development.     

细胞凋亡的研究现状

综述了细胞凋亡的概念和意义，若干种调控细胞凋亡的基因及其作用和研究这一课题的临床价值。     

Effect of P15INK4b expression on the cell cycle and G1 phase related regulatory genes in human melanoma cells

Using the transfeetion teehnique. P15INK4b was introduced into P15INk4b gene deleted human melanoma A375 cells,and a cell model MLED6 overexpressing P15INK4b WAS CONSTRUCTED.Comparing with the control cells MLC2,MLEK6cells in G1phase increased by 11%,but those in Sphase decreased by 15%by FCM.By the method of thymidine(TdR)and N2O arresting,the proportions of synchronized Mphase cells of MLEK6 ana MLC23 were measured and found to be 89.1% and 76.8%respectively ,and the cells in G1phase were 74.3% for MLID6 AND 76. 4% forMLC2.The result of3 H-TdR incorporation indicated that the transition of G1/Sof MLEK6 cell was delayed 2h as compared with that of MLC2 cells,and incorporation rate also decreased.The observation on exprissions of some G1/ S-resates relatory rigusating genes showed that in MLIK6 cells the protein leves of P27KIPI increased with the decreasing expressions of cyclinD1,cyclinE and c-myc,especially cyclinD1 in late G1phade.The expression of cyclinE obviously decreased at G1/S transition ,and c-myc wad inhibited throughout all the process of G1 S phase.All the risults suggest that P15INK4b can delayG1/S transition of MLEK6 cells by inhibiting the cell cycle engine ,and by increasing the expression of Cdk ingibitor P27KIPI in different stages of G1 phase.     

白桦脂酸体外抗肿瘤的活性和机制

研究白桦脂醇（Betulin）和白桦脂酸（Betulinic Acid, BA）对3种癌细胞株的增殖抑制作用以及白桦脂酸诱导MCF 7细胞毒的分子机制. 应用结晶紫染色方法对白桦脂醇和白桦脂酸对肿瘤细胞的生长抑制作用进行筛选； 应用RT-PCR技术检测MCF 7细胞p21 mRNA, p53 mRNA, Bcl 2 mRNA和Bax mRNA的表达. 结果表明, 白桦脂醇和白桦脂酸有显著抑制肿瘤细胞生长的作用, 且有浓度依赖性; 白桦脂酸能够诱导MCF 7细胞凋亡, 并诱导其p21 mRNA和p53 mRNA表达增高, 但对其Bcl 2 mRNA和Bax mRNA 的表达无影响.     

视科研为生命一部分的青年学者——访上海师范大学贾能勤教授

贾能勤（上图）是我最新认识的一位启明星朋友．一开始吸引我的是他此次入选启明星跟踪计划的课题：“多功能碳纳米管生物载体系统及其促进肿瘤细胞凋亡研究”，但对他这些年的求学研究之路有所了解后，我觉得有某种相见恨晚的感觉。尽管我俩年龄有很大差距，但彼此对一些问题的看法、处世的方式有共同之处，如对启明星计划作用的理解，对交叉学科重要性的认识，对科学文献的浏览、阅读兴趣，等等。