黑尾胡蜂毒液抗菌肽的分离纯化及功能研究

由于病原微生物对众多传统抗生素药物的耐药性问题日益突显,因此寻找新型的抗菌药物显得尤为紧迫,多肽类制剂因其具有广谱的抗菌活性,已成为克服病原微生物耐药性的潜在候选药物.从黑尾胡蜂毒液中分离并鉴定了一种具有抗菌活性的多肽,命名为VT-1.VT-1对临床致病菌均表现出广谱的抗菌活性.此外,VT-1对人红细胞显示出微弱的溶血活性.     

钙拮抗剂对高血压并左室肥厚患者的血浆脑钠素的影响

目的:研究长效钙拮抗剂治疗高血压并左室肥厚对血浆脑钠素(BNP)的影响,为临床选择降压药提供更客观的依据.方法:①比较原发性高血压并左室肥厚、无左室肥厚及健康对照组BNP浓度的差别;②选择左室肥厚组作为随访对象,予以长效钙拮抗剂治疗(6±3)月,比较BNP、左室质量指数(LVMI)在治疗前后的变化.结果:①高血压并左室肥厚组血浆BNP水平显著高于高血压无左室肥厚组和健康对照组(P＜0.001);②高血压患者血浆BNP与LVMI呈正相关关系(相关系数r=0.551,P＜0.001);③使用长效钙拮抗剂治疗高血压并左室肥厚患者(6±3)个月,血浆BNP水平和LVMI均显著降低(P＜0.001).结论:长效钙拮抗剂治疗高血压并左室肥厚患者能显著降低患者BNP水平,能够逆转左室肥厚.     

SH2域与磷酸化多肽相互作用的连续溶剂模型研究

使用连续溶剂模型方法研究了SH2结构域与磷酸化多肽pYXXX(X为20种常见氨基酸残基中的任意一种)之间的相互作用.首先计算了已知的SH2域-磷酸化酪氨酸多肽复合物之间的结合能,理论计算的结果与实验测得的亲合能之间的相关系数为0.91,验证了理论模型的正确性.然后,用该模型方法计算了SH2域与pYXXX之间的结合能,分析了磷酸化酪氨酸多肽pYXXX中 1, 2, 3位置上残基对结合能的影响.结果表明 2, 3位置上残基的变化对结合能影响较大, 2位置上带负电的残基和 3位置上的疏水性残基有利于SH2域与pYXXX之间的相互作用,这与实验结果一致.     

A designed amphiphilic peptide containing the silk fibroin motif as a potential carrier of hydrophobic drugs

The amphiphilic peptide is becoming attractive as a potential drug carrier to improve the dissolvability of hydrophobic drugs in an aqueous system; thus, facilitating drug uptake by target cells. Here, we report a novel designed amphiphilic peptide, Ac-RADAGAGARADAGAGA-NH₂, which was able to stabilize pyrene, a hydrophobic model drug we chose to study in aqueous solution. This designed peptide formed a colloidal suspension by encapsulating pyrene inside the peptide-pyrene complex. Egg phosphatidylcholine (EPC) vesicles were used to mimic cell bilayer membranes. We found that pyrene was released from the peptide coating into the EPC vesicles by mixing the colloidal suspension with EPC vesicles, which was followed by steady fluorescence spectra as a function of time. A calibration curve for the amount of pyrene released into the EPC vesicles at a given time was used to determine the final concentration of pyrene released into the lipid vesicles from the peptide-pyrene complex. The release rate of the peptide-pyrene complex was calculated to quantify the transfer of pyrene into EPC vesicles.     

心钠素与妊高征

对心钠素与妊高妊的关系及活性心钠素Ⅲ治疗妊高征的效果进行综述。     

β-淀粉样蛋白寡聚化模拟研究进展

AD主要病理改变之一为老年斑。聚集的β淀粉样多肽（Aβ）纤维是老年斑构成核心，也导致AD患者神经退行性病变和认知障碍。Aβ神经毒性主要来自于可溶性Aβ寡聚体。但是关于Aβ及其寡聚体的毒性性质存在不同的研究观点，而且Aβ的聚集机制和产生毒性的机制尚不十分明确。Aβ的折叠和聚集是一个非常复杂的过程，在研究寡聚化问题时，经典...     

活性肽抗皮肤老化作用研究进展

活性肽是近年来功能性食品的研究热点. 随着人口老龄化和社会活动的增加，延缓皮肤老化越来越受到人们的关注，活性肽在此方面展现了一定的应用前景，尤其是胶原蛋白肽在美容方面有大量应用和研究报道. 文章结合皮肤老化的机理，从肽的吸收和转运、肽在抗皮肤老化实验研究等方面综述了活性肽的抗皮肤老化作用研究进展.     

Heat shock proteins in autoimmune disease. From causative antigen to specific therapy?

Heat shock proteins (hsp) are highly conserved from bacteria to man. Bacterial hsp, with approximate molecular weights of 60 kDa (hsp60), are immunodominant antigens that are immunologically cross-reactive with their mammalian counterparts. Hsp molecules are therefore useful in studies of fundamental questions concerning immune responses to foreign as opposed to self antigens. The finding that immune responses to hsp are associated with both experimentally-induced and spontaneous autoimmune diseases in animals has prompted intensive research to assess the role of bacterial hsp as the etiological agents involved in the development of autoimmune diseases. Recent evidence from animal models of autoimmune disease has clearly demonstrated the involvement of hsp in both the pathogenesis and the immunoregulation of autoimmune diseases. Studies with arthritogenic and diabetogenic T cell clones have identified immunogenic epitopes of hsp. These have been shown to ameliorate adjuvant arthritis in Lewis rats, and insulin-dependent diabetes mellitus (IDDM) in non-obese diabetic (NOD) mice. Such studies may have important therapeutic implications for the future treatment of human autoimmune disease.Dedicated to Professor Hermann A. Moser on the occasion of his 71st birthday.     

酵母密码子编码的α降钙素基因相关肽基因的化学合成和克隆

记叙了α降钙素基因相关肽基因的化学合成和克隆.该多肽的合成基因序列是根据已知的氨基酸序列,选用酵母偏爱的密码子,由计算机辅助设计而成的,共136个碱基对.全基因分为六个寡核苷酸片段在DNA合成仪上合成.这些片段混合退火后,用T4DNA连接酶一次装配成功,并克隆到M13mp18载体上.经核酸序列分析,证明所得克隆的核苷酸顺序与设计完全一致.