大弹涂鱼松果体形态结构研究

鱼类松果体是传达外界信息到体内的主要传感器之一,其分泌的褪黑激素与鱼类生殖活动密切相关.为了阐明大弹涂鱼松果体形态结构与其功能的关系,运用组织细胞学方法研究了大弹涂鱼松果体的形态结构.结果表明,大弹涂鱼松果体位于端脑和中脑之间,由松果体柄和终囊两部分组成,具有背囊结构.松果体柄细长,细胞排列紧密,柄中有空腔,开口与第三脑室相通;松果体终囊膨大成梨形,且有空腔,腔内有皱褶,终囊顶部表面有黑色素细胞;背囊位于终囊下方,形状不规则,其囊壁向囊腔内反复折叠,形成许多复杂、盘旋曲折的内褶,背囊内部可见血细胞.研究结果为将来揭示大弹涂鱼半月周期产卵的机制提供了基础资料.     

基于FPGA的测温系统的设计

介绍了单总线数字温度传感器DS18B20的特性及工作原理,给出了由DS18B20与FP-GA组成的测温系统的硬件电路和软件设计及提高测量精度的方法.通过对实际温度的测量及与专用测温仪的比较,发现该系统具有数据准确、接口简单、精度高、抗干扰能力强、工作稳定可靠等特点.     

基于J2EE的中职考务管理系统的设计实现

本文对中职考务管理工作进行需求分析,提出了系统设计方案并予以实现.系统采用J2EE架构,实现了考试信息的网上输入、统计、查询、打印等需求,具备了自动安排考试、权限管理、手动安排考试的冲突检测功能,实现了教师、学生、考场、科目等教学资源的集中网络化处理,对提高安排考试效率具有重要的意义.     

微波无极紫外灯光降解罗丹明B水溶液

自制微波无极紫外灯(MWUVL)发出的185 nm UV占总辐射量的12%,是普通低压汞灯的1.7倍左右.利用自制MWUVL研究了罗丹明B(RhB)水溶液的光降解.研究表明,酸性条件有利于RhB的光降解;ρ初始≥60mg.L-1的RhB溶液在光降解过程中有沉淀析出;pH=4.0的100 mg.L-1RhB溶液光照75 m in后,析出的沉淀质量接近初始RhB质量的一半,脱色率达99.4%,CODC r下降77.4%;RhB的降解主要是185 nm直接光解和OH自由基氧化的共同作用的结果.     

鳞翅目昆虫细胞抗菌肽的诱导、筛选及抗菌活性的研究

选取3种离体培养的鳞翅目昆虫细胞系,采用热灭活的大肠杆菌诱导的方法,诱导其产生抗菌肽,并进行抗菌活性检测、诱导动力学研究及Tricine SDS-聚丙烯酰胺凝胶电泳检测分析．结果筛选到1株诱导抗菌活性较高的昆虫细胞系——粉纹夜蛾BTI-Tn-5B1细胞系,并发现该粉纹夜蛾5B1细胞在诱导后16h产生了1个分子量大约为8000的抗菌肽,抗菌活性检测表明其对金黄色葡萄球菌（Sta phylococcus aureus）、大肠杆菌（Escherichia coli K12D31）、德氏卑沙门氏菌（Salmonella derby）均有不同程度的抑制作用,其中特别是对革兰氏阴性菌——大肠杆菌K12D31和德氏卑沙门氏菌有较强的抑菌活性．     

平台匹配规则对B2B交易的影响

针对交易型B2B平台不同匹配规则对B2B交易的影响展开研究。基于讨价还价理论,分别刻画了B2B平台4种匹配规则下交易双方的讨价还价行为,并深入分析了不同竞争情形下的均衡结果。通过对比,研究平台不同匹配规则在不同竞争情形中对B2B交易的影响。研究表明,B2B平台上的交易价格取决于平台的交易匹配规则;匹配规则对B2B平台上的交易额(GMV)的影响与平台收取的佣金系数息息相关;匹配规则对B2B平台上买卖双方利润的影响因买方的竞争情形及最终产品的替代程度而有所差异。此外,匹配规则对交易价格、交易额和买卖方利润的影响在不同的竞争情形中表现出不同的强度。     

The start-up of biohydrogen-producing process by bioaugmentation in the EGSB reactor

Expanded granular sludge bed (EGSB) reactor and bioaugmentation were employed to investigate biohydrogen production with molasses wastewater. The start-up experiments consisted of two stages. In the first stage (0～24d) seeded with activated sludge, the butyric acid type-fermentation formed when the initial expanding rate, organic loading rate (OLR), the initial redox potential (ORP) and hydraulic retention time (HRT) were 10%, 10.0 kg COD/(m3·d), - 215 mV and 6.7 h, respectively. At the beginning of the second stage on day 25, the novel hydrogen-producing fermentative bacterial strain B49 (AF481148 in EMBL) were inoculated into the reactor under the condition of OLR 16. 0 kg COD/(m3·d), ORP and HRT about - 139 mV and 6.7 h, respectively, and then the reaction system transformed to ethanol-type fermentation gradually with the increase in OLR. When OLR, ORP and HRT were about 94.3 kg COD/(m3·d), -250 mV and 1.7 h, respectively, the system achieved the maximum hydrogen-producing rate of 282.6 mL H2/L reactor· h and hydrogen percentage of 51%～53% in the biogas.     

Effects of medium nutrition on cell growth and isocamptothecin A and B production by suspension cell culture of Camptotheca acuminata

The effects of initial sucrose concentration, nitrate to ammonium ratio, total N concentration and phosphate concentration in medium on cell growth and isocamptothecin A and B synthesis by suspension call culture of Camptotheca acuminata were investigated in 250 mL shake flasks. 30 g L^-1 sucrose concentration was beneficial to secondary metabolites synthesis. The cell growth and metabolites synthesis were also affected by the ratio of NO3^-/NH4^＋ , and nitrate was tavourable for cell growth. The maximum dry weight was achieved when nitrate was used as the sole N souree. The effect of total initial N on the cell cultures was also investigated with NO3^-/NH4^＋ ratio of 1 ： 2. The final dry cell weight was similar throughout culture period and 50 mM initial N was favourable for secondary metabolite synthesis. 50 mM initial phosphate concentration facilitated both cell growth and secondary metabolites synthesis.     

基于B/S结构的在线测试系统的设计与实现

介绍了基于B/S结构的在线测试系统的基本功能和实现方法,给出了系统的应用设计,完成了系统模型的实现.系统具有良好的学生测试和自动评价功能,利用网络数据库的优势解决了群体测试的整体评价.     

WDR34 is a novel TAK1-associated suppressor of the IL-1R/TLR3/TLR4-induced NF-κB activation pathway

Toll-like receptors (TLRs) act as sensors of microbial components and elicit innate immune responses. All TLR signaling pathways activate the nuclear factor-kappaB (NF-κB), which controls the expression of inflammatory cytokine genes. Transforming growth factor-β-activated kinase 1 (TAK1) is a serine/threonine protein kinase that is critically involved in the activation of NF-κB by tumor necrosis factor (TNFα), interleukin-1β (IL-1β) and TLR ligands. In this study, we identified a novel protein, WD40 domain repeat protein 34 (WDR34) as a TAK1-interacting protein in yeast two-hybrid screens. WDR34 interacted with TAK1, TAK1-binding protein 2 (TAB2), TAK1-binding protein 3 (TAB3) and tumor necrosis factor receptor-associated factor 6 (TRAF6) in overexpression and under physiological conditions. Overexpression of WDR34 inhibited IL-1β-, polyI:C- and lipopolysaccharide (LPS)-induced but not TNFα-induced NF-κB activation, whereas knockdown of WDR34 by a RNA-interference construct potentiated NF-κB activation by these ligands. Our findings suggest that WDR34 is a TAK1-associated inhibitor of the IL-1R/TLR3/TLR4-induced NF-κB activation pathway. D. Gao and R. Wang contributed equally to this work.