表面涂覆活性凝胶聚合物暂堵剂的研究与应用

以聚乙烯醇、骨胶和苯甲酸为固相材料,采用具有表面活性的单体和丙烯酰胺、丙烯酸钠进行共聚得到的活性聚合物对粉状固体表面进行包覆,通过有机硅改性处理得到一种水溶性暂堵剂WRP,测试了暂堵剂的悬浮性、水溶性以及抗温性能,通过驱替实验测试了暂堵和解堵性能。实验结果表明,该暂堵剂水溶率大于95%,可耐260℃高温,悬浮分散性能好,在岩心中注入8倍孔隙体积的0.4%暂堵剂后,暂堵率与水驱解堵率均大于95%,突破压力高达6.2 MPa。并将其应用于赵凹油田的安2117井进行了现场实验,加入暂堵剂后压力上升6.3 MPa,达到暂堵效果和解堵效果,实现了裂缝的暂堵转向。     

PolSAR图像的两级分块最优加权滤波方法

针对多极化合成孔径雷达（polarimetric synthetic aperture radar, PolSAR）图像的相干斑抑制问题,提出一种两级分块的最优加权滤波方法。该方法在分块加权法的块处理基础上,采用边缘检测模板对非同质类子块进行二次分类和处理,提高了滤波参数的估计精度。实验结果表明,该方法不仅保持了块处理简单、运算量小的优点,而且在斑点抑制和边缘保持方面更优于最优加权法和分块加权法。     

原发性高血压运动疗法的研究综述

运动疗法是原发性高血压非药物疗法中非常重要的一种,其疗效日益受到关注。本文对近年来有氧运动和力量训练治疗原发性高血压的研究现状进行综述,探讨运动疗法治疗原发性高血压的作用及其可能性机制,为更好的发挥运动疗法在高血压防治中的作用提供一定支持。     

The molecular mechanism of photodynamic therapy to fibrosis: Regulation on the pyridinoline cross-link formation in collagen

Fibrotic processes in tissues are characterised by an excessive accumulation of collagen containing increased levels of pyridinoline cross-links, which has been taken as an important criterion to diagnose the fibrosis. Hypocrellin B （HB） is a natural photosensitizer with excellent photodynamic activity but very low dark-toxicity. In the current work, main purpose was to clarify if photodynamic action of HB could be applied to regulating the level of pyridinoline cross-link in collagen. To use gelatin as a model collagen, it was observed that the pyridinoline cross-link formation was effectively degraded by HB photodynamic action in the gelatin, suggesting the level of pyridinoline cross-link formation in collagen may be photodynamically regulated to a normal level. Furthermore, mechanism of the photodynamic action of HB was studied.     

hPNAS-4腺病毒载体的构建及其体外抗肿瘤作用

[摘要] 目的：构建携带人的凋亡相关新基因PNAS-4(hPNAS-4)的重组腺病毒,并观察其感染人肺癌A549细胞所引起的hPNAS-4过表达对体外肿瘤细胞凋亡的影响。方法：用RT-PCR从293A细胞中克隆hPNAS-4编码区cDNA,将其酶切后连接至pENTR11载体上,再通过pENTR11与腺病毒骨架载体pAd/CMV/V5-DEST之间的同源重组作用将hPNAS-4基因片段重组至pAd/CMV/V5-DEST上,最后经293细胞的包装扩增后得到携带hPNAS-4基因的重组腺病毒;将重组腺病毒体外感染人肺癌A549细胞;用RT-PCR检测感染细胞中hPNAS-4的过表达情况;通过MTT、流式细胞术及DNA Ladder分别检测感染细胞的增殖与凋亡情况。结果：从293A细胞中中克隆到hPNAS-4全长cDNA并成功构建腺病毒表达载体Ad-hPNAS-4,经测定其滴度为：2.4×108 pfu/ml,感染人肺癌A549细胞后：经RT-PCR测得其mRNA表达明显上调,MTT检测结果为细胞增殖受到明显抑制,流式细胞术测得细胞凋亡率明显升高,琼脂糖凝胶电泳显示其基因组DNA有明显的梯状条带（DNA ladder）;结论：hPNAS-4腺病毒载体感染人肺癌A549细胞后,发现hPNAS-4过表达对肿瘤细胞的生长有明显的抑制和诱导凋亡作用,为今后研究其分子机制以及hPNAS-4腺病毒载体应用于肿瘤动物实验和肿瘤基因治疗提供实验资料。     

针灸治疗糖尿病周围神经病变临床研究概况

本文综述了近5年来针灸治疗糖尿病周围神经病变(DPN)的相关临床报道,例举了单一疗法及综合疗法,并结合临床研究,证明针灸是治疗DPN的有效方法,且操作方便,无毒副作用.     

义65井区高温低渗油藏储层伤害机理分析

义65井区沙三段九砂组油藏属中等水敏性和速敏性的地层,油藏为轻质油－稀油油藏,原油具有低密度、低黏度和高凝固点等特点,地层水总矿化度较高,一般为1100～19900mg/L,水型为NaHCO₃型。研究了义65井区高温低渗储层伤害机理,从地层特性、注入水水质、悬浮物堵塞、游离油和乳化油滴、结垢、细菌及以往增产措施等方面评价了可能对储层造成的损坏及损坏程度。义65井区地层的水敏性、速敏性和低渗透性是注水井损害的内在因素,注水井中悬浮固相颗粒、含油量和细菌严重超标,水质不合格是注水井损害的外在因素。     

中医综合疗法治疗寒凝血瘀型原发性痛经40例

本研究观察了中医综合疗法治疗寒凝血瘀型原发性痛经的临床疗效。将80例寒凝血瘀型原发性痛经患者随机分为两组,其中治疗组40例以中药内服、外敷、针灸联合治疗;对照组40例以少腹逐瘀胶囊治疗。结果表明：治疗组总有效率97．6％;对照组总有效率83．3％。两组总有效率比较,有显著差异性P〈0．05。因此中医综合疗法治疗寒凝血瘀型原发性痛经有良好疗效。     

沙疗温度及血液灌注对膝关节温度场和应力场的影响

为探究沙疗过程中沙体温度及血液灌注率对于人体膝关节部位温度场分布以及骨骼热应力的影响,在生物传热学以及经典力学的理论基础上,通过运用将膝关节部位的计算机断层扫描(computed tomography,CT)影像数据导入3D逆向建模软件Mimics建立三维模型,并将模型导入COMSOL软件并进行传热数值模拟的方法来研究了沙疗过程中膝关节温度场以及热应力的变化规律.结果表明:在45℃沙体环境下沙疗30 min,膝关节骨骼的温度和热应力主要集中在髌骨和胫骨上,而在胫骨的长骨部位尤为集中.由骨骼热应力变化曲线可以知道骨骼平均热应力在沙疗20 min时达到顶峰,在20～30 min出现下降趋势,然后在30 min以后随着时间增加开始逐渐减退.可见合适的沙疗时间在20 min左右为最佳,但不超过30 min为宜.在整个沙疗过程中,血液灌注率对于人体的温度场以及应力场起着至关重要的调节作用.     

Development of a nano-immunomodulator encapsulating R837 and caffeine for combined radio-/immunotherapy against orthotopic breast cancer

Immunotherapy is an ascendant approach in cancer treatment. It shows more pronounced effects on killing cancer cells in a specific manner in particular against metastasis more than traditional techniques, such as chemotherapy or surgery. However, tumor immunosuppression limits the response of the immune system to cancer development. In this study, we developed a lipid-based nanocarrier doubly loaded with imiquimod (R837), a toll-like receptor 7 agonist, and caffeine, an adenosine receptor antagonist. This R837/caffeine loaded nanocarrier served as a nano-immunomodulator (RC-nIM) for combination treatment with radiotherapy (RT) against orthotopic breast cancer. RT-induced immunogenic cell death facilitated the production of tumor antigen and elicited the immune response in corporation with R837-medaited activation of antigen-presenting cells (APCs) while RC-nIMs being adopted. Additionally, caffeine, an adenosine analog, can successfully compete with adenosine in the tumor. The tumor-bearing mice that received RT together with RC-nIMs experienced the best antitumor effects and exhibited higher levels of T cells and APCs within the tumor; the growth of secondary tumors was also limited. This work serves as a proof-of-concept study for the development of a new immunotherapy strategy against cancer.