循环伏安法对聚合物LB膜屏蔽效应的研究

利用电化学循环伏安法研究了沉积在ＩＴＯ导电玻璃电极上液晶聚合物ＬＢ膜的屏蔽效应．通过测量各种成膜条件下循环伏安图的变化，结合Ｘ－射线衍射和紫外光谱的结果，对ＬＢ膜的微细结构进行了探讨．     

上消化道病内镜微波组织凝固疗法的临床应用与评价

本文报告应用内镜微波组织凝固疗法(EMCT)治疗胃息肉、胃癌、消化性溃疡三种疾病21例、39次治疗。提出了溃疡面搔刮加微波凝固治疗难治性溃疡、及EMCT联合内镜下局部注射化疗治疗进展期胃癌等方法。胃息肉13例27枚及Ⅱa型早期胃癌1例与消化性溃疡3例全部达到治愈。85.2％的胃息肉EMCT一次治疗成功,Ⅱa型早癌一次成功,消化性溃疡2例一次成功。进展期胃癌4例均达到肿块缩小、狭窄的胃腔扩大与自觉症状改善的效果。鉴于疗效显著,认为三种疾病均适用本疗法;并就EMCT的适应症、使用限度和治疗目的作了讨论;展望了以EMCT作为早期胃癌经内镜根治疗法手段之一的远景。     

Peptide aptamers: exchange of the thioredoxin-A scaffold by alternative platform proteins and its influence on target protein binding

Peptide aptamers have emerged as powerful new tools for molecular medicine. They can specifically bind to and functionally inactivate a given target molecule under intracellular conditions. Typically, peptide aptamers are generated by screening a randomized peptide expression library, displayed from the Escherichia coli thioredoxin A (TrxA) protein. Here, we transferred peptide moieties from defined TrxA-based peptide aptamers to alternative scaffold proteins, such as the green fluorescent protein and staphylococcal nuclease. Yeast and mammalian two-hybrid assays as well as in vitro binding analyses show that the TrxA scaffold can be a major determinant for the binding of peptide aptamers. In addition, we demonstrate that TrxA can correctly display peptide sequences that correspond to the binding domains of natural interaction partners. Therefore, sequence analyses of TrxA-based peptide aptamers, isolated by two-hybrid screening from randomized expression libraries, should also be useful to find cellular binding partners for a given target protein, by homology. Received 1 August 2002; received after revision 17 September 2002; accepted 19 September 2002 RID="*" ID="*"Corresponding author.     

Progress in non-viral gene delivery systems fabricated via supramolecular assembly

Gene delivery systems are one of key issues that limit the development of gene therapy. The novel non-viral gene delivery systems fabricated via supramolecular assembly have begun to show increasing promising and applications in gene therapy due to its suitable nanometric size,controllable structure and excellent biocompatibility. In this review, the fundamental and recent progress of non-viral gene supramolecular assembly is reviewed. Artificial viruses the future direction of non-viral gene delivery systems are also described.     

Timid算法、Aggressive算法以及FCA的性能分析

首先引入了动态信道分配Timid算法和Aggressive算法．然后通过一定的仿真条件假设，对系统采用Timid算法和采用固定信道分配(FCA)算法的性能作了分析，同时又对采用Aggressive算法和采用FCA算法的性能也作了分析．并且对采用Timid算法和Aggressive算法的性能进行了比较，可以看到只有选择一种有效的动态信道分配机制才能使系统性能得到极大的改善。     

Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma

Vectors pose most pivotal problem of gene therapy[1]. Because of the high transfection efficiency both in vitro and in vivo, the viral vector has been employed in 70% clinical trials of gene therapy (http://www.wiley.co.uk/ genmed/clinical). However, thei…     

BNCT物理剂量Monte Carlo程序系统——MCDB及算法

硼中子俘获治疗(BNCT,boron neutron capture therapy)规划软件系统MCDB(Monte Carlo dosimetry inbrain),包括医学前处理、Monte Carlo物理剂量计算和后处理。采用中心点方法确定网格的材料和密度,并自动生成Monte Carlo输入文件。MCDB借鉴并发展了一套网格几何下的快速粒子径迹算法,取得了与MCNP程序一致的剂量计算结果,计算速度较MCNP程序提高2.7~3.5倍。MCDB可进行并行计算,具有线性加速比,能够满足BNCT临床对计算时间和精度的要求。     

Polarization of immunity induced by direct injection of naked sequence-stabilized mRNA vaccines

In the context of developing a safe genetic vaccination strategy we tested and studied globin-stabilized mRNA-based vaccination in mice. This vaccination strategy has the advantages of genetic vaccination (easy production, adaptability to any disease and inexpensive storage when lyophilized), but not the drawbacks of DNA vaccination (long-term uncontrolled expression of a transgene, possibility of integration into the host genome and possible induction of anti-DNA antibodies). We report here that injection of naked -globin untranslated region (UTR)-stabilized mRNA coding for -galactosidase is followed by detectable translation in vivo. In addition, we show that such a vaccination strategy primes a T helper 2 (Th2) type of response which can be enhanced and shifted to a Th1-type immune response by application of recombinant granulocyte/macrophage colony-stimulating factor 1 day after mRNA injection. Our data demonstrate that the administration of globin UTR-stabilized mRNA is a versatile vaccination strategy that can be manipulated to fit the requirement of antiviral, antibacterial or antitumor immunity.Received 14 June 2004; received after revision 19 July 2004; accepted 9 August 2004     

拯救灵魂:穆旦宗教选择的精神指向

作为一个现代知识分子,诗人穆旦有着"自我折磨"与"折磨人"的双重人格品质,他在对中国现实的深入思考中敏锐地洞察到:在中国人身上有着一种沉疴已久的精神疾病--"灵魂饥饿",这是传统的信仰价值体系崩溃后,新的信仰价值体系未曾建立时出现的一种病症.穆旦针对"灵魂饥饿"这一现实,通过对中外文化的对比考察,找到了一条救治这一精神疾病的药方--基督信仰,借以医治自己和别人.     

我国古代"情志相胜,以情胜情"的心理疗法及其对现代心理治疗的影响

我国中医学源远流长,其"情志相胜,以情胜情"的心理治疗理念、相应的具体治疗方法及案例,对于分析现代人的心理疾病、并针对性地进行临床治疗具有重要的作用.