昆虫几丁质酶的进化与保守性质的比较基因组学研究

昆虫几丁质酶家族属于第18家族糖苷键水解酶,主要参与昆虫蜕皮、细胞增殖和免疫等生理过程。在7个物种基因组数据中通过BLASTP和PSI-BLAST的方法获得131条chitinase和chitinase-like的同源蛋白质,利用隐马尔科夫算法,找到了几丁质蛋白的保守模体区域。通过进化树分析,将所选的蛋白分为9组,与糖苷水解酶18催化结构域和几丁质结合结构域的特性一致。通过PAML软件计算出几丁质酶的正向选择位点,发现了豌豆蚜虫中的A.pisum xp003243674.1为一条新的几丁质酶蛋白。对深入研究昆虫几丁质酶结构与功能很有帮助。     

太钢脱硫脱硝吸附塔移动单元异地平台组对施工工法

异地平台组对施工技术将部分高空作业变为地面作业,降低了施工作业的风险,并且在现场不具备条件的情况下可以提前开始作业,缩短了施工周期。该技术适用于除尘、环保类设备的施工。     

Molecular imprinted polymer with positively charged assistant recognition polymer chains for adsorption/enrichment of low content target protein

Here, we introduce a new type of molecular imprinted polymer （MIP） with immobilized assistant recognition polymer chains （ARPCs） to create effective recognition sites and with bacterial cloned protein as template for adsorbing the low content target protein from cell extract. In this work, cloned pig cyclophilin 18 （pCyP18）, a peptidyl-prolyl cis/trans-isomerase, was used as template. The template protein was selectively assembled with ARPCs from their library, which consists of numerous limited length polymer chains with randomly distributed recognition sites of positively charged amino groups and immobilizing sites. These assemblies were adsorbed by porous microsphers and immobilized on them. After removing the template, binding sites complementary to the target protein in size, shape and the position of recognition groups were exposed, and their confirmation was preserved by the cross-linked structure. The synthesized MIP was used to adsorb the cellular pCyP18, and its proportional content was enriched more than hundred times. The extended experiment on imprinting bovine serum albumin （BSA） with ARPCs shows that this method is also suitable for large protein.     

ON MANAGERIAL DECISION PROBLEM OF THE AUCTION SITES

This paper studies one managerial decision problem of the auction sites： how to combine penalties with commissions？ It is shown that when there exists secondary auction market, the auction whose penalty is a linear function of the seller＇s commission will be more efficient than the auction with only commissions but no penalties. It has also been proven that using this strategy can maximize the expected revenue for the seller, the auction site and their total expected revenues.     

巴式铜钲的考古发现与研究

在战国到两汉时期,巴族地区的窖藏和墓葬中出土了数量较多的铜钲,从形制特征看,钲腔瘦长,近直筒形,圆管状甬,有的为六棱或八棱柱状甬,有的饰环钮,于弧,内腔有的有四条音脊。按照我国古代的礼乐制度,铜钲与錞于是连同使用的,除用于军旅外,还用于祭祀和宴乐。     

河南境内东周城址濒危现状考察

据统计,河南境内分布有173座东周古城,目前保存较为完整的仅有20座。依其地上城垣的完整程度可分为6类。这些古城址受到自然、人为的破坏,已经濒临毁灭。自然因素对这些古城址的破坏是渐进的,而人为因素则是对城址的彻底破坏。     

山西省地质遗迹资源及保护问题研究

山西省地质遗迹资源丰富，涵盖六大类型．典型的区域地质背景和特殊的自然环境条件造就其鲜明的特色．但目前保护利用中存在很多问题，其中遗迹破坏严重、资源利用率与资源占有量不协调等问题尤为突出．文章本着“积极保护，合理开发”的原则，提出一些现实可行的保护建议，旨在实现山西地质遗迹的可持续发展．     

基于DBSCAN算法的郑洛地区史前聚落遗址聚类分析

为了解决判别聚落群过于依赖考古专家人工划分的问题,以郑洛地区新石器时代聚落遗址为例,采用基于密度的DBSCAN(density-based spatial clustering of applications with noise)算法对聚落遗址进行空间聚类研究。通过对郑洛地区四个文化时期聚落遗址的分布分析,发现郑洛地区的主体聚落群从研究区东部的嵩山以南地区,转移到郑洛地区中部的伊洛河流域,并且在伊洛河流域长期定居下来,不断发展扩大;大型聚落遗址主要分布在主体聚落群里,除了裴李岗文化时期部分大型聚落较孤立;从仰韶文化后期到龙山文化时期,聚落遗址分布呈主从式环状分布格局;大多数聚落群的走向都和河流分布一致。研究表明,利用DBSCAN算法进行聚落遗址聚类是可行的,通过聚类得到郑洛地区新石器时代四个文化时期聚落遗址的分布特征。     

Direct observation of hydrogen-t rapping sites in newly developed high-strength mooring chain steel by atom probe tomography

Hydrogen-trapping sites were directly observed by using a three-dimensional atom probe(3DAP) in high-strength mooring chain steel.Three typical 3DAP datasets show that trapping hydrogen is mainly enriched in retained austenite,and secondarily in carbides.In addition,these interfaces between ferrite and carbide are segregated by phosphorus,but do not admit hydrogen.