铜对三种豆科植物萌发及早期生长发育的抑制效应

采用水培实验研究了Cu2+对种子萌发和胚根胚芽伸长的抑制效应;采用盆栽实验研究了土壤中不同含量Cu对植物地上、地下部分长度和质量以及叶绿素含量的影响.结果表明:质量浓度≥400mg.L-1的Cu2+水溶液对三种植物种子的萌发产生显著的抑制作用,质量浓度越高阻抑越明显,对紫花苜蓿的抑制效应小于红三叶和沙打旺;对胚根伸长的抑制作用大于芽.过量Cu2+可显著降低植物叶绿素的含量,并对地上部分的生长产生明显的抑制作用;对紫花苜蓿及红三叶根系长度和质量有明显的促进作用,而对沙打旺则有明显的抑制作用.总体来看,紫花苜蓿对Cu污染的耐受程度大于红三叶和沙打旺.     

重组碱性成纤维细胞生长因子ELISA检测方法的建立

小鼠或家克经重组bFGF免疫后产生高滴度的抗血清,用此血清建立了间接ELISA检测方法,检测bFGF的灵敏度达10ng/ml.     

广州地区1995—1997年HIV抗体检测情况分析

为了解献血员人群ＨＩＶ感染情况，我们对１９９５～１９９７年献血员ＨＩＶ抗体检测情况进行了分析。方法：献血员ＨＩＶ抗体的初筛试验采用抗－ＨＩＶ抗体ＥＬＩＳＡ法，ＨＩＶ抗体初筛试验阳性者用蛋白印迹法（ＷＢ）进行确认。结果：３年内共检测１４００２８人次，初筛ＨＩＶ抗体阳性１８例献血员，并全部经ＷＢ法确认为ＨＩＶ抗体阳性。其中１５例为男性，３例女性，均为来自外省的流动人口。１８例ＨＩＶ抗体阳性献血员中，ＨＣＶ抗体阳性检出率为８３３％（１５／１８），ＨＢｓＡｇ阳性检出率为６１１％（１１／１８），ＨＢｓＡｇ和ＨＣＶ抗体阳性检出率为３３３％（６／８）。结论：在献血员人群中ＨＩＶ抗体阳性检出率呈上升趋势，为此献血员ＨＩＶ抗体筛查对预防和控制输血传播ＨＩＶ具有重要意义。ＨＩＶ与ＨＢＶ和ＨＣＶ具有较高的重叠感染率     

鸭肝炎I型琼脂扩散试验的研究

本试验用不同的鸭肝炎病料，通过氯仿去脂和反透析法进行提纯浓缩，研究鸭肝炎的琼脂扩散。结果显示用抗原检测抗体或用抗体（血清）检测病毒病原，均可达到80%的阳性检出率。试验中还发现，试验最佳的凝胶配方为pH为7.2,ω(NaCl)为8%和琼脂糖的质量分数为1%，病雏鸭肝浓缩抗原最为理想。     

邢钢焦化厂水质稳定剂

介绍了根据邢钢焦化厂的水质，经静态阻垢试验，旋转挂片腐蚀试验筛选水质稳定剂配方的过程及实际应用情况。试验及结果表明，LG－1配方具有良好的阻垢缓蚀性能。     

用于图象增强的侧抑制网络模型的仿真比较

侧抑制原理及侧抑制网络在工程上有着广泛的应用，在用于图像增强方面，需要从侧抑制网络模型，抑制系数分布、抑制野范围三方面考虑，选择合适的模型和参数，从而达到 突出边框，加强反差的效果，根据侧抑制数学 模型的分类规则，本文基于前人根据生理实验建立的数学模型综合出一种新的模型并选择双峰高斯曲线作为抑制系数分布函数，通过对这些模型及参数的计算机仿真实验，比较了它们在网络运行速度及边框突出程度方面的差异。     

求解多目标0/1背包问题的克隆选择算法

提出一种用于求解多目标 0/1 背包问题的新算法.新算法将抗体群中的抗体分为支配抗体和非支配抗体代替传统算法中对所有个体分配适应度值,解决了多目标优化问题中解的多样性的问题.先通过克隆操作实现全局择优,得到分布较广的Pareto-前端,接着采用免疫基因操作提高算法的局部搜索能力,同时采用抗体修正操作对由免疫基因等操作产生的不可行解进行修正,保证抗体在可行解范围内,并实现局部搜索.该算法与已有算法相比能更好地保持解的多样性、均匀性以及收敛性.仿真实验表明,新算法所得的 Pareto-前端分布最广,所得的解能较好地收敛到 Pareto-前端,并且将均匀性评价指标降低到1%以下.     

树枝状大分子的合成及其阻垢性能

采用发散合成法,合成了以乙二胺为核的1.0代树枝状大分子聚酰胺-胺(PAMAM 1.0),用静态法测定了树枝状大分子在高硅溶液中对胶体硅的阻垢性能.结果表明,PAMAM 1.0在高硅溶液中对胶体硅有较好的阻垢效果,且阻垢效果受pH值、溶解硅初始质量浓度和PAMAM 1.0用量的影响。在pH值为7.0,溶解硅初始质量浓度为500?mg/L的溶液中,PAMAM 1.0有效质量浓度为60mg/L时,30h后溶液中的溶解硅质量浓度能够保持为406.24mg/L,远高于使用其他阻垢剂时的质量浓度.作为一种新型的水处理剂.PAMAM 1.0显示了良好的应用前景。     

Insight into the covalent grafting of organic films onto carbon steel surfaces for protection

The novel use of p-nitrophenyldiazonium tetrafluoroborate salt(GG salt)as a protectant that is electrochemically grafted onto carbon steel has been investigated in0.05 mol L-1H2SO4and 5 wt%NaCl solutions using various corrosion monitoring techniques,such as electrochemical impedance spectroscopy,potentiodynamic polarisation,infrared spectra and scanning electron microscopy measurements.The electrochemical study reveals that this compound is a mixed inhibitor that predominantly controls the cathodic reaction.The surface-grafted film decreases the double-layer capacitance and obviously increases the charge transfer resistance relative to a bare carbon electrode.The values of inhibition effect remain nearly unchanged with an increase in temperature range of 298–318 K.The aryl diazonium is covalently bonded on the steel surface,causing a slight decrease in the apparent activation energy.Overall,the surface-grafted films exhibit excellent inhibition performance in acid and saline solutions within the studied temperature range.     

Aberrant DNA methylation in cloned ovine embryos

By using the approach of immunofluorescence staining with an antibody against 5-methylcytosine （5MeC）, the present study detected the DNA methylation patterns of cloned ovine embryos. The embryos derived from in vitro fertilization were also examined for reference purpose. The results showed that： （1） during the preimplantation development, cloned embryos displayed a similar demethylation profile to the fertilized embryos; that is, the methylation level decreased to the lowest at 8-cell stage, and then increased again at morulae stage. However, methylation level was obviously higher in cloned embryos than in stage-matched fertilized embryos, especially at 8-cell stage and afterwards; （2） at blastocyst stage, the methylation pattern in cloned embryos was different from that in fertilized embryos. In cloned blastocyst, inner cell mass （ICM） exhibited a comparable level to trophectoderm cells （TE）, while in in-vitro fertilized blastocyst the methylation level of ICM was lower than that of TE, which is not consistent with that reported by other authors. These results indicate that DNA methylation is abnormally reprogrammed in cloned embryos, implying that aberrant DNA methylation reprogramming may be one of the factors causing cloned embryos developmental failure.