Presence of hepcidin-25 in biological fluids: Bile,ascitic and pleural fluids

AIM: To examine body fluids such as ascitic fluid (AF),saliva,bile and pleural effusions for the presence of hepcidin using a novel radioimmunoassay (RIA).METHODS: Serum samples were collected from 25 healthy volunteers (mean age: 36 ± 11.9 years,11 males,14 females).In addition bile was obtained from 12 patients undergoing endoscopic retrograde cholangiopancreatography (mean age: 66.9 ± 16.7 years,M:F = 5:7).Saliva was collected from 17 healthy volunteers (mean age: 35 ± 9.9 years,M:F = 8:9).Pleural and AF...     

Application of surface plasmon resonance in screening adsorbents and explaining adsorption phenomena using model polymers

A surface plasmon resonance (SPR) technique was developed as an online screening method to identify effective adsorbents for the peptide NH₂-Cys-Thr-Trp-Trp-COOH (CW-4). To validate the SPR technique, several linear polymers containing amino acid residues were synthesized as models of their corresponding adsorbents. SPR screening of all the linear polymers demonstrated that the linear polymer containing phenylalanine residues (PPhe) exhibited the highest affinity for CW-4 among the polymers tested. In accordance with the screening results from SPR, the adsorbent containing phenylalanine (APhe) exhibited the highest adsorption capacity for CW-4. An interesting observation was that the amounts of CW-4 adsorbed on 3 adsorbents were quite small, even though both SPR and isothermal titration calorimetry (ITC) experiments indicated the presence of interactions between CW-4 and the three polymers. A kinetic analysis performed using SPR technology suggested such a phenomenon was ascribed to the poor stability of the CW-4/polymer complexes; that is, CW-4 associates with the polymers quickly, but it also dissociated quickly. The combined results suggested that SPR was a promising tool to identify the optimal adsorbent for peptides, analyze the interactions contributing to adsorption and explain adsorption phenomena using polymer models.     

The effect of corticotropin-releasing factor and pro-opiomelanocortin-derived peptides on the phagocytosis of molluscan hemocytes

The effect of corticotropin-releasing factor (CRF) and pro-opiomelanocortin (POMC)-derived peptides on hemocyte phagocytosis in two molluscs,Planorbarius corneus andViviparus ater was studied. The peptides and related fragments examined are those which have been shown to influence hemocyte motility in the two species. The results obtained revealed that the effects on phagocytosis are not directly correlated with previous findings on cell motility. Furthermore, the mode of action of an individual peptide could be species-specific and dose-dependent. The relationships between peptides, locomotion and phagocytosis in these molluscs are discussed.     

沉淀二氧化硅载银抗菌剂的制备及其抗菌性能

以沉淀二氧化硅为载体，吸附硝酸银后，在500℃下灼烧，制备了具有抗菌功能的线银沉淀二氧化硅抗菌剂，并检验了其抗菌性能；还对抗菌剂的抗菌机理进行了初步探讨。测试结果表明，沉淀二氧化硅的载银量在7％以上时，所制备的抗菌剂对绿脓杆菌、大肠杆菌和枯草杆菌的杀菌率达到100％，具有优异的抗菌性能，可用于抗菌塑料、抗菌食品包装、抗菌卫生纸和抗菌纺织制品等领域。     

反应性抗菌整理剂的合成及其抗菌性能研究

本文以2,4,4′-三氯-2′-羟基二苯醚以及其磺化产物SDP为抗菌有效组分,合成了含一氯均三嗪活性基的反应性抗菌整理剂;并作了波谱分析(UV,IR,MS)和元素分析。这类抗菌整理剂可方便地用于纤维素纤维的抗菌整理,且整理后的织物具有优异的抗菌性能和耐洗坚牢度。例如:按AATCC-100法,经反应性抗菌整理剂2RSDP整理后的织物对金黄色葡萄球菌、大肠杆菌和绿脓杆菌的减菌率分别达到98.5％,98.86％和96.67％;经60℃、100次以上皂洗其减菌率仍保持在90％左右。因而,具有实际应用、开发的价值。同时,本文也初步探讨了所合成的反应性抗菌整理剂的结构与抗菌性能关系。     

Antibacterial activity in the egg mass of a sea hare

Summary The eggs of a sea hare,Aplysia kurodai, contained antibacterial factors which probably play a role in the defense of eggs against bacterial infection. The active factors were composed of several heat-labile proteins, unrelated to lysozyme, and were produced in the albumen gland.     

壳聚糖季铵盐晶体的制备及其抑菌、柔软剂性能

采用异相法制备了水溶性的羟丙基三甲基氯化铵壳聚糖 (HACC)晶体 ;测定了它对枯草芽孢杆菌等微生物生长的抑制效果 ;并研究了由HACC复配而成的微乳液在织物柔软处理中的应用性能。     

等离子体对壳聚糖整理棉织物效果的影响

研究等离子体预处理技术对棉织物壳聚糖功能整理效果的影响.比较3种不同的整理方法.第1种是用柠檬酸及壳聚糖的混合溶液进行一浴整理;第2种是先用柠檬酸溶液再用壳聚糖溶液进行两浴处理;第3种是使用等离子体预处理织物后再经两浴法整理.使用酸性橙Ⅱ对整理后织物染色并测试K/S值.通过测试织物大肠杆菌和金黄色葡萄球菌的抗菌率考察其抗菌性.结果表明,经等离子体预处理后再经两浴法整理的织物的K/S值以及抗菌率较一浴法或两浴法都有所提高,对大肠杆菌和金黄色葡萄球菌的抗菌率高达100%.     

多步沉淀法制备纳米无机复合抗菌粉体

目的研究一种以银、钛和锡为组分的新型纳米无机复合抗菌粉体的制备工艺。方法采用多步沉淀法,并通过单因素实验和正交实验确定最优工艺条件。结果最终产品的基本化学组成式为Ag0.03Sn0.16Ti0.35P0.11O0.34;产率达95.5%;粉体平均粒径50 nm;无外加光照、粉体浓度80mg/L时,30 min内其对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抗菌率均达100%;粉体颜色浅,耐候性好。结论该方法制备的粉体具有较强的广谱抗菌效果,解决了银系抗菌剂易变色、对真菌作用效果不佳及钛系抗菌剂必须有光照才能起作用等问题。     

3-羟基-4-甲酸乙酯己啶盐酸盐的体外抗菌活性和毒性

试管双倍稀释法测定1.6-二-(N5-3-羟基-4-甲酸乙酯苯基-N1二胍)己烷盐酸盐对金黄色葡萄球菌、大肠杆菌、鸡白痢沙门氏杆菌的体外抑菌作用,并以现在临床上常用的氯己啶盐酸盐作为对照药物,采用急性毒性和蓄积实验测定它的毒性.结果表明这种新化合物显示出较好的抑菌活性,毒性试验表明这种新化合物是低毒的.