Pharmacological versus binding analysis of receptor systems: How do they interplay? Myometrial cell receptors for oxytocin as a paradigm

Summary Binding studies in various biological systems frequently indicate the presence of several binding sites for a biologically active ligand. They differ in their affinity for the ligand in question, binding capacity, and Hill coefficient, which suggests differences in the mechanisms of the binding site-ligand interactions. Identification of the true receptors (sites initiating a cellular response) appears to be difficult. Three clusters of binding sites for oxytocin were found on rat myometrial cells. The oxytocin receptor seems to be linked to the medium-affinity site; the cooperation between the high-and medium-affinity sites in eliciting the uterotonic response seems likely, but lacks experimental proof. Dose-response analysis in partially irreversibly inhibited uterus preparations, the method of equipotent doses (Furchgott-Bursztyn method), and structure-activity analysis of oxytocin-like peptides acting as competitive inhibitors of oxytocin, turned out to be suitable for pharmacological analysis of this receptor system.     

Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells^1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently.     

多晶3C-SiC薄层的淀积生长及结构性能分析

采用ＨＦＣＶＤ生长法,以较低生长温度,在Ｓｉ（１１１）衬底上淀积３Ｃ－ＳｉＣ（１１１）薄层。用ＸＲＤ、ＶＡＳＥ、ＸＰＳ等分析手段研究了薄层的结构、光学常数、组分及化学键等性能。ＸＲＤ显示薄层具有明显的择优取向特征。ＶＡＳＥ测量出薄层的折射率为２．６８６,光学常数随深度的变化曲线反映出薄层的多层结构。ＸＰＳ深度剖面曲线表明薄层中Ｓｉ／Ｃ原子比符合ＳｉＣ的理想化学计量比,其能谱证明Ｃ１ｓ与Ｓｉ２ｐ成键形成具有闪锌矿结构的３Ｃ－ＳｉＣ。     

用光反射测试方法检测的硅抛光片和外延片表面的图谱

利用检测半导体硅抛光片及硅外延片表面缺陷的光反射法（魔镜法）［1］,观测到了二十余种硅抛光片及硅外延片表面缺陷的图样．本文报道了部份图样．     

化学镀Ni-P合金层组织结构与性能的研究

对非晶态Ｎｉ－Ｐ合金镀层的组织结构和性能进行研究,结果表明：非晶态Ｎｉ－Ｐ合金镀层以层片状沉积,表面具有胞状结构．镀层的磷含量及热处理温度对Ｎｉ－Ｐ合金层的组织结构有影响,同时对非晶态Ｎｉ－Ｐ合金镀层在不同介质中的耐蚀性进行了研究．     

软磁Fe基纳米微晶的穆斯堡尔谱研究

对纳米软磁合金Ｆｅ７３Ｃｕ１Ｎｂ１．５Ｍｏ２ｓＩ１２．５Ｂ１０的制备态和各退火态的室温Ｍｏｓｓｂａｕｅｒ谱研究,发现非晶相的超精细内场存在高场和低场两部分,     

钨对Ni-W-P合金镀层的结构和性能的影响

通过现代测试技术研究了化学沉积Ｎｉ－Ｗ－Ｐ合金镀层的结构、热稳定性、硬度、耐蚀性和抗冲刷腐蚀性能的影响．结果表明：Ｎｉ－Ｗ－Ｐ合金镀层的结晶状态主要由磷含量决定,但钨却使镀层形成非晶态结构所需的磷含量降低,同时钨的存在大大提高了合金镀层的热稳定性、硬度、耐蚀性和抗冲刷腐蚀性能．     

脆江蓠营养细胞超微结构的研究

脆江蓠营养细胞超微结构观察结果表明：营养细胞每一色素体内含有一条围周类囊体、４～１０条平行类囊体和一些质体小球．平行类囊体数目与细胞所处的部位有关．内质网呈环形片层结构．同心圆膜状体具有１或２个核心．色素体在末端进行分裂,分裂时平行类囊体数目增多．幼色素体被膜直接内陷形成平行类囊体．以后发育出围周类囊．     

纳米结构硅研究的发展动态*

本文综合归类了目前制备纳米结构硅的主要方法：等离子体化学气相沉积．激光诱导化学气相沉积和热蒸发法（制纳米晶硅）及电化学腐蚀法（制多孔硅）,给出各种方法的典型参数及其对纳米硅结构的影响,分析了纳米硅结构特征,比较分析了各种方法制备的纳米硅的光学性能,如光学能隙Ｅｏｐｔｇ,光致／电致发光谱峰位波长、半高宽及影响因素等,并对纳米硅研究的发展前景进行展望．     

螺旋藻藻蓝蛋白和藻多糖对人体外周血淋巴细胞功能的影响

从螺旋藻中提取藻蓝蛋白（ＳＰＣ）和多糖（ＳＰＳ）,研究其对人体外周血淋巴细胞功能的影响。实验结果表明,ＳＰＣ和ＳＰＳ能够促进ＰＨＡ刺激淋巴细胞转化的作用,其中ＳＰＳ的促进作用有剂量依存关系。ＳＰＣ和ＳＰＳ能恢复Ｔ细胞受环磷酰胺损伤后Ｅ玫瑰花环形成能力,特别是对活性Ｅ花环（Ｅａ）的形成能力有较好的恢复作用。