Effect of GbKTN1 from Gossypium barbadense on cell elongation of fission yeast（Schizosaccharomyces pombe）

The GbKTN1 gene was isolated from 10 DPA fiber cells of Gossypium barbadense using 5′RACE/3′RACE.Full-length cDNA of this gene is 2006 bp, including a 113 bp of 5′untranslated region, a 1563 bp of an open reading frame(ORF), and a 327 bp of 3′untranslated region (excluding the stop codon TAA). The ORF of GbKTN1 encodes a 521-amino acid protein with a predicted size of 55 kD. Near C-terminal of the deduced protein there is a putative ATP binding site between amino acid residues from 233 to 414. Southern blot analysis indicated that the GbKTN1 was a single copy gene in G barbadense. Combining semi-quantitative RT-PCR with Southern blot hybridization revealed that GbKTN1 expressed in all the organs detected such as roots, stems, leaves and fibers. However, the mRNA of GbKTN1 was the most abundant in fiber cells, while it was the lowest in leaves. The GbKTN1 cDNA was transformed into S. pombe to verify its function on cell elongation. Results showed that most yeast cells over expressing GbKTN1 gene were elongated dramatically with an average length increase of 2.18 times than that of the non-induced cells. Even the morphology of some yeast cells appeared irregularly. To the best of our knowledge this is the first evidence that KTN1 is correlated with cell elongation in vivo.     

苦瓜子蛋白的体外抗氧化活性研究

测定了苦瓜子蛋白提取过程中不同提取物的体外抗氧化活性,从而获得不同目标活性的蛋白组分.结果表明,90%饱和度的硫酸铵沉淀蛋白的柱纯化组分抗氧化活性较强,30%的次之,60%的最弱,三种饱和度硫酸铵沉淀纯化得到的苦瓜子蛋白在不同抗氧化体系中存在抗氧化活性差异.     

NO and H2O2 induced by Verticillium dahliae toxins and its influence on the expression of GST gene in cotton suspension cells

Nitric oxide （NO） and hydrogen peroxide （H2O2） have been shown to be important signaling molecules that participate in the regulation of several physiological processes. In particular, they have significant role in plant resistance to pathogens by contributing to induction defense genes. Here, whether NO and H2O2 participate in the resistance responses against Verticillium dahliae toxins （VD-toxins） and their effects on the expression of GSTgene are studied. The results reveal that NO and H2O2 are produced as part of a complex network of signals that respond to VD-toxins and may converge to function both synergistically and independently by inducing resistant responses. GSTgene is potentially involved in the resistance mechanism in the cotton suspension cells. NO induces the expression of GSTgene independently of H2O2. H2O2 may be a more potent signal in the resistance responses against VD-toxins.     

体育环境的改变对老龄锻炼者心理影响差异的实验研究

为了研究不同体育环境对老龄锻炼者心理影响的差异,运用心理实验的方法,对在两种不同环境中经常参加晨跑的16名老年人,先后进行三次心理测试(前测、后测与长效后测)。对三次测量的结果研究表明:不同体育环境对老年锻炼者的认知能力以及抑郁、焦虑、紧张等情绪状态产生的影响具有显著性差异,良好的室外运动环境对调节老年人的情绪状态、提高老年人的认知能力,促进心理健康具有积极的意义。     

体育环境的改变对老龄锻炼者心理影响差异的实验研究

为了研究不同体育环境对老龄锻炼者心理影响的差异,运用心理实验的方法,对在两种不同环境中经常参加晨跑的16名老年人,先后进行三次心理测试(前测、后测与长效后测).对三次测量的结果研究表明:不同体育环境对老年锻炼者的认知能力以及抑郁、焦虑、紧张等情绪状态产生的影响具有显著性差异,良好的室外运动环境对调节老年人的情绪状态、提高老年人的认知能力,促进心理健康具有积极的意义.     

高速逆流色谱分离纯化波棱瓜子中化学成分

采用高速逆流色谱对波棱瓜子乙酸乙酯提取物进行分离纯化,以石油醚-乙酸乙酯-甲醇-水(体积比2:6:3:5)为溶剂,上相为固定相,下相为流动相,流速2 mL/min,转速850 r/min,共分离得到2个高纯度化合物,经过高效液相色谱检测,纯度均高于95%。该方法简便、快速、节省溶剂,可以对波棱瓜子中的化学成分进行快速有效的分离纯化。     

钩藤种子发芽影响因素研究

【目的】研究钩藤（犝狀犮犪狉犻犪狉犺狔狀犮犺狅狆犺狔犾犾犪）种子的发芽条件,为钩藤种子检验和种子质量标准的制定提供参考.【方法】采用单因素试验法研究种子来源、发芽温度、光照和抑菌处理等因素对钩藤种子发芽的影响.【结果】钩藤种子发芽的适宜温度为20～25℃;黑暗不利于钩藤种子的发芽,每天应固定给予部分光照;无菌水处理的种子发芽率和发芽势最高,发霉种子也较少;来源于广西融水县的种子发芽率和发芽势最高.【结论】钩藤种子整体发芽率较低,不同温度、光照、消毒处理及不同来源的种子的发芽率有显著差异.     

不同寄主植物对棉铃虫生长发育影响因素的分析

为了探索棉花叶片、加工番茄叶片、加工番茄青果和加工番茄红果对棉铃虫幼虫生长发育的影响,测定棉花叶片和加工番茄的果实等组织器官的主要物质含量,分析影响棉铃虫幼虫生长发育的因素。结果表明:以加工番茄叶片饲喂的棉铃虫上幼虫发育历期最短16.04 d、幼虫存活率最高54.44%,蛹羽化率最高83.58%,以加工番茄红果发育历期最长26.17 d,幼虫成活率最低3.67%,蛹羽化率最低0.00%。因此,4种食料中棉铃虫的适合度为:加工番茄叶片棉花叶片加工番茄青果加工番茄红果。棉花叶片、加工番茄叶片和果实中的营养成分差异较大,其中加工番茄叶片的可溶性蛋白含量最高为15.14%、C/N为0.91;加工番茄红果可溶性蛋白含量最低为3.04%、C/N为9.65。因此,植物组织内的可溶性糖含量低、C/N小、含水量和可溶性蛋白含量高有利于棉铃虫生长发育。