阿托伐他汀对高温高湿应激大鼠心肌细胞GRP78和CHOP表达的影响

探讨阿托伐他汀对高温高湿应激大鼠心肌细胞内内质网应激相关因子葡萄糖调节蛋白78(glucose-regulated proteinof 78kD,GRP78)和C/EBP环磷酸腺苷反应元件结合转录因子同源蛋白(CAAT/enhancer binding protein homologous protein,CHOP)表达的影响。将60只雄性SD大鼠随机分为对照组、高温高湿组、阿托伐他汀组,每组20只。按实验时间(2 w、4 w、6w、8 w)的不同,各组又分为4个亚组,每个亚组5只大鼠。用颈动脉插管法测定各组大鼠的平均动脉压(MAP)。心脏超声心动图检测左心室形态结构。用免疫组织化学法检测心脏GRP78和CHOP的表达水平。①高温高湿各亚组的MAP随着实验时间的延长呈逐渐递增的趋势,高温高湿4 w、6 w、8 w亚组的MAP均高于相应的对照组和阿托伐他汀组(P<0.05)。②高温高湿组心肌细胞中GRP78在2 w即有低水平表达,高于对照组,但无统计学意义。随着实验时间的延长,高温高湿组GRP78表达量不断增加,6 w达到最大值,8 w表达减弱。高温高湿4 w、6 w、8 w亚组GRP78表达量均高于相应对照组和阿托伐他汀组,有统计学意义(P<0.05)。③高温高湿组2 w、4 w、6 w、8 w亚组CHOP表达量组间比较有统计学意义(P<0.05),8 w亚组表达达到最高值。高温高湿组2 w、4 w亚组与相应对照组比较无统计学意义(P>0.05);高温高湿组6 w、8 w亚组与相应对照组和阿托伐他汀组比较有统计学意义(P<0.05)。说明高温高湿应激可引起心肌细胞内质网应激反应,导致GRP78表达及CHOP表达的不对称增加,提示高温高湿应激可引起心肌细胞的损害。阿托伐他汀可以减轻内质网应激,逆转高温高湿应激所致的心肌细胞的损伤作用,对心肌细胞有保护作用。     

急性心肌梗死患者外周血T淋巴细胞和NK细胞的变化

目的：研究急性心肌梗死患者外周血 Ｔ 淋巴细胞的变化。方法：采用流式细胞仪技术,观察１５ 例急性心肌梗死患者外周血 Ｔ 淋巴细胞 Ｃ Ｄ２ ＋ 、 Ｃ Ｄ３ ＋ 、 Ｃ Ｄ４ ＋ 、 Ｃ Ｄ８ ＋ 、 Ｃ Ｄ１６ ＋ 、 Ｃ Ｄ２５ ＋ 、 Ｃ Ｄ２７ ＋ 、 Ｃ Ｄ５７ ＋ 变化,并与１２ 例正常对照组比较。结果： Ｃ Ｄ２ ＋ 、 Ｃ Ｄ３ ＋ 、 Ｃ Ｄ１６ ＋ 、 Ｃ Ｄ２７ ＋ 、 Ｃ Ｄ５７ ＋ 无显著性变化; Ｃ Ｄ４ ＋ 明显减少, Ｃ Ｄ８ ＋ 明显增加, Ｃ Ｄ４ ＋ ／ Ｃ Ｄ８ ＋ 比值显著降低; Ｃ Ｄ２５ ＋ 显著降低。结论：急性心肌梗死患者 Ｔ 淋巴细胞亚群功能紊乱,细胞免疫功能受抑制。     

取代芳烃对三种水生生物急性毒性的自相关拓扑研究

基于原子i的生物点价（δi^1）和自相关函数。建构自相关拓扑指数（^mB）及其逆指数（^mB,）．其中的^1B、^1B,与26种取代芳烃对3种水生生物急性毒性的线性方程如下：（1）发光菌：-lgEC50=2．1843＋0．0203^1B-0．0080^1B′,R=0．9581 （2）大型蚤：-lgEC50=2．1980＋0．0193^1B＋0．0872^1B′,R=0．9667 （3）呆鲦鱼：-lgLC50=2．2633＋0．0247^1B-0．6280^1B′,R=0．9371以上的QSAR模型给出的计算结果优于文献方法。     

血清Ig类、BNP和D-二聚体检测与急性心肌梗死(AMI)的相关性研究

目的探讨急性心肌梗死(AMI)患者血清中IgA,IgG,IgM,BNP和D-二聚体(D-D)浓度的变化与AMI的关系.方法利用免疫比浊法和荧光免疫法对60例AMI患者血清中IgA,IgG,IgM,BNP和D-D浓度进行测定,并与30例正常对照组比较.结果AMI患者血清中的IgA,IgG,IgM,BNP和D-D浓度明显高于正常对照组,与疾病严重程度呈正相关.结论监测AMI患者血清IgA,IgG,IgM,BNP和D-D浓度的变化,对AMI严重程度评估和疗效观察有重要的临床意义.     

水体中盐酸环丙沙星的风险等级分析

分析了抗生素类药物盐酸环丙沙星对水中不同食物链水生生物包括小球藻、发光菌、大型蚤、孔雀鱼的影响,其急性毒性EC₅₀或者LC₅₀数值分别为20.61,63.27,135.15,365.00 mg·L^-1。毒性作用规律结果显示,随着生物链等级的升高,生物对环丙沙星的耐受程度升高。将综合评价因子(PEC/PNEC)作为环境风险表征参数,对大连开发区污水处理厂排水及大连湾沿海养殖区中存在的盐酸环丙沙星进行风险分析,发现盐酸环丙沙星在沿海养殖区水域中的环境风险高于污水处理厂排水。小球藻的综合评价因子数值范围最大,可作为评价水体中盐酸环丙沙星残留风险等级的生物标志物。     

3种常见有机磷农药对麦瑞加拉鲮鱼幼鱼的急性毒性

开展了三唑磷、乙酰甲胺磷、增效水胺硫磷对麦瑞加拉鲮鱼幼鱼的急性毒性试验,结果表明:三唑磷对麦瑞加拉鲮鱼幼鱼24h,48h,72h,96h的半致死浓度分别为0.080mg/L,0.065mg/L,0.060mg/L,0.050mg/L;乙酰甲胺磷对麦瑞加拉鲮鱼幼鱼24h,48h,72h,96h的半致死浓度分别为113.783mg/L,114.451mg/L,113.366mg/L,113.214mg/L;增效水胺硫磷对麦瑞加拉鲮鱼幼鱼24h,48h,72h,96h的半致死浓度分别为0.736mg/L,0.549mg/L,0.469mg/L,0.280mg/L。麦瑞加拉鲮鱼幼鱼对三唑磷、乙酰甲胺磷、增效水胺硫磷96h安全质量浓度分别为0.005mg/L、0.028mg/L和11.321mg/L,3种有机磷农药的毒性大小依次为三唑磷、乙酰甲胺磷、增效水胺硫磷。最后,还就三唑磷、乙酰甲胺磷、增效水胺硫磷对麦瑞加拉鲮鱼幼鱼的急性致毒效应特征以及麦瑞加拉鲮鱼幼鱼对三唑磷、乙酰甲胺磷、增效水胺硫磷的安全浓度进行了评价。     

The outbreak pattern of the SARS cases in Asia

The severe acute respiratory syndrome (SARS) caused tremendous damage to many Asia countries, especially China. The transmission process and outbreak pattern of SARS is still not well understood. This study aims to find a simple model to describe the outbreak pattern of SARS cases by using SARS case data commonly released by governments. The outbreak pattern of cumulative SARS cases is expected to be a logistic type because the infection will be slowed down due to the increasing control effort by people and/or due to depletion of susceptible individuals. The increase rate of SARS cases is expected to decrease with the cumulative SARS cases, as described by the traditional logistical model, which is widely used in population dynamic studies. The instantaneous rate of increases were significantly and negatively correlated with the cumulative SARS cases in mainland of China (including Beijing, Hebei, Tianjin, Shanxi,the Autonomous Region of Inner Mongolia) and Singapore. The basic reproduction number R0 in Asia ranged from 2.0 to 5.6 (except for Taiwan, China). The R0 of Hebei and Tianjin were much higher than that of Singapore, Hongkong, Beijing, Shanxi, Inner Mongolia, indicating SARS virus might have originated differently or new mutations occurred during transmission. We demonstrated that the outbreaks of SARS in many regions of Asia were wall described by the logistic model, and the control measures implemented by governments are effective. The maximum instantaneous rate of increase, basic reproductive number, and maximum cumulative SARS cases were also calculated by using the logistic model.     

Detecting myocardial ischemia with 2-D CVIB imaging method--an in vivo animal experiment study

A 2-D cyclic variation of integrated backscatter (CVIB) imaging method was established for detecting myocardial ischemia. To demonstrate the feasibility and validity of this method, animal experiments were conducted. Acute myocardial ischemia was induced by occluding left anterior descending coronary artery in 10 anesthetized open-chest dogs. While scanning the normal hearts and the ischemic hearts with a B scanner, digital radiofrequency data were acquired by a real-time acquisition system in synchronism. The offline analysis to the radio-frequency signal with the 2-D CVIB imaging method was performed to verify the consistency between the imaging result and the design of the experiment. In addition, 4 dogs in experiment were treated with the heart pacemaker in order to investigate the influence of changing in heart rate on the detection of ischemic myocardium with the proposed method. The experimental result showed that the 2-D CVIB imaging method succeeded in detecting the ischemic myocardium and is a new non-invasive way for the cardiologists to both quantitatively and visually evaluate the contractile performance of the myocardium.     

瑞替普酶溶栓治疗急性心肌梗死的疗效观察

目的:研究ST段抬高的急性心肌梗死(STEMI)发病后不同时间溶栓的疗效和不良反应.方法:对86例住院的STEMI患者用瑞替普酶行溶栓治疗,依据发病到溶栓开始的时间将86例患者分为三组:A组≤lh (n=28),B组>lh,但<6h(n=36),C组>6h,但≤12h(n=22),观察梗塞相关动脉(IRA)的开通率、5周病死率和溶栓副作用.结果:IRA开通率:A组92.9%(26/28),B组86.1%(31/36),C组45.5%(10/22),C组与A、B两组差异性显著(P<0.01),A、B两组间无显著性差异(P>0.05).病死率:A组、B组和C组的5周病死率分别为0%(0/28),2.77%(1/36)和4.54%(1/22).A、B两组间无显著性差异[0%(0/28)vs 2.77%(1/36),P>0.05],A组与C组有显著性差异[0%(0/28)VS 4.54%(1/22),P<0.01],B组与c组有显著性差异[2.77%(1/36)V8 4.54%(1/22),P<0.01],三组中均无包括脑出血在内的重大出血并发症.结论:瑞替普酶溶栓治疗STEMI疗效好、安全性高.     

脑梗死所致癫痫持续状态的抢救及护理

目的：了解脑梗死引起癫痫持续状态时的抢救及护理措施．方法：对36例癫痫持续状态病人给予抗癫痫、降颅压、吸氧、纠正电解质紊乱,并配合各种护理措施,密切观察病情变化及时给予对症处理．结果：36例中34例经有效的抢救及护理,痊愈出院．结论：脑梗死所致癫痫持续状态经及早救治,精心护理,可以痊愈．